Initiator Caspases Antibody Sampler Kit【科瑞奥博】

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Initiator Caspases Antibody Sampler Kit

货号： 12675T 基本售价： 4822.0 元规格： -

产品信息

概述
货号	12675T
目标/特异性	Each antibody in the Initiator Caspases Antibody Sampler Kit recognizes endogenous levels of its respective target. Caspase-3 (8G10) Rabbit mAb recognizes full-length (35 kDa) and the large fragment (17/19 kDa) of caspase-3 resulting from cleavage at aspartic acid 175. Caspase-8 (1C12) Mouse mAb recognizes full length (57 kDa), the cleaved intermediate p43/p41, and the p18 fragment of caspase-8. Caspase-9 (C9) Antibody recognizes full-length caspase-9, as well as the large fragments resulting from cleavage at Asp315 and Asp330. Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb recognizes caspase-9 only when cleaved at Asp330. Caspase-2 (C2) Mouse mAb recognizes procaspase-2, as well as its 14 and 12 kDa cleaved fragments.

性能
供应商	CST
背景	Apoptosis is a regulated physiological process leading to cell death. Caspases, a family of cysteine acid proteases, are central regulators of apoptosis. Initiator caspases (including 2, 8, 9, 10 and 12) are closely coupled to proapoptotic signals, which include FasL, TNF-α, and DNA damage. Once activated, these caspases cleave and activate downstream effector caspases (including 3, 6 and 7), which in turn cleave cytoskeletal and nuclear proteins such as PARP, α-fodrin, DFF and lamin A; inducing apoptosis (1,2).
存放说明	-20C
参考文献	1 . Muzio, M. et al. (1996) Cell 85, 817-27. 2 . Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4. 3 . Budihardjo, I. et al. (1999) Annu Rev Cell Dev Biol 15, 269-90. 4 . Boldin, M.P. et al. (1996) Cell 85, 803-15. 5 . Cohen, G.M. (1997) Biochem. J. 326, 1-16. 6 . Fernandes-Alnemri, T. et al. (1996) Proc Natl Acad Sci U S A 93, 7464-9. 7 . Chinnaiyan, A.M. et al. (1995) Cell 81, 505-12. 8 . Liu, X. et al. (1996) Cell 86, 147-157. 9 . Nicholson, D. W. et al. (1995) Nature 376, 37-43. 10 . Nagata, S. (1997) Cell 88, 355-65. 11 . Boldin, M.P. et al. (1995) J Biol Chem 270, 7795-8. 12 . Li, P. et al. (1997) Cell 91, 479-489. 13 . Hsu, H. et al. (1995) Cell 81, 495-504. 14 . Zou, H. et al. (1999) J. Biol. Chem. 274, 11549-11556. 15 . Vincenz, C. and Dixit, V.M. (1997) J Biol Chem 272, 6578-83. 16 . Kischkel, F.C. et al. (2001) J Biol Chem 276, 46639-46. 17 . Srinivasula, S.M. et al. (1998) Mol Cell 1, 949-57. 18 . Wang, J. et al. (2001) Proc Natl Acad Sci U S A 98, 13884-8. 19 . Hsu, H. et al. (1996) Cell 84, 299-308. 20 . Li, H. et al. (1997) J Biol Chem 272, 21010-7. 21 . Tinel, A. and Tschopp, J. (2004) Science 304, 843-6. 22 . Dirsch, V.M. et al. (2004) Oncogene 23, 1586-93. 23 . Castedo, M. et al. (2004) Oncogene 23, 4362-70. 24 . Paroni, G. et al. (2001) J Biol Chem 276, 21907-15.

参考图片

Western blot analysis of extracts from SKW6.4 cells, untreated or anti-Fas-treated (1 µg/ml), and Jurkat cells, untreated or etoposide-treated (25 µM), using Caspase-8 (1C12) Mouse mAb.

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.

Western blot analysis of HeLa (human) and NIH/3T3 (mouse) cell extracts, untreated and treated with 1 μM staurosporine (3 hr) in vivo, using Caspase-3 (8G10) Rabbit mAb.

Immunoprecipitation of cleaved caspase-3 from Jurkat cell extracts untreated (control) or treated with etoposide (25uM 5hrs) (apoptotic) using Caspase-3 (8G10) Rabbit mAb, and western probed with the same antibody.

Western blot analysis of extracts from Jurkat cells, untreated or etoposide-treated (5 hr, 25μM), using Caspase-2 (C2) Mouse mAb.

Western blot analysis of extracts from Jurkat cells (human), L929 cells (mouse), and C6 cells (rat), untreated or treated with staurosporine or cytochrome c as indicated, using Caspase 9 (C9) Mouse mAb.

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence^® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence^® Caspase-3 siRNA I (+), using Caspase-3 (8G10) Rabbit mAb and p42 MAPK Antibody #9108. Caspase-3 (8G10) Rabbit mAb confirms silencing of caspase-3 expression, while the p42 MAPK Antibody is used to control for loading and specificity of caspase-3 siRNA.

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence^® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence^® Caspase-3 siRNA II (+), using Caspase-3 (8G10) Rabbit mAb and α-Tubulin (11H10) Rabbit mAb #2125. Caspase-3 (8G10) Rabbit mAb confirms silencing of caspase-3 expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of caspase-3 siRNA.

Western blot analysis of extracts from HeLa and Jurkat cells, untreated or treated with Staurosporine #9953 (1 μM, 3 hr) or etoposide (25 μM, overnight), using Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb (upper) or total Caspase-9 Antibody (Human Specific) #9502 (lower).

Western blot analysis of HeLa (human) and NIH/3T3 (mouse) cell extracts, untreated and treated with 1 μM staurosporine (3 hr) in vivo, using Caspase-3 (8G10) Rabbit mAb #9665.

未处理或在体内经过1 μM staurosporine (3 hr) 处理的HeLa (human) 和 NIH/3T3 (mouse)细胞的细胞提取物，使用Caspase-3 (8G10) Rabbit mAb 兔单抗#9665 进行western blot实验。

未处理或经过staurosporine 或 cytochrome处理的Jurkat 细胞 (人源)、L929 细胞（小鼠源）以及C6 细胞(大鼠源)的细胞提取物，使用Caspase 9 (C9) Mouse mAb 鼠单抗#9508进行western blot分析。

Western blot analysis of extracts from HeLa and Jurkat cells, untreated or treated with Staurosporine #9953 (1 μM, 3 hr) or etoposide (25 μM, overnight), using Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb #7237 (upper) or total Caspase-9 Antibody (Human Specific) #9502 (lower).

未处理或Staurosporine #9953 (1 μM, 3 hr) 或 etoposide (25 μM, 过夜)处理的HeLa细胞和Jurkat细胞的细胞提取物，使用Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb 兔单抗#7237 (上) 或total Caspase-9 Antibody (Human Specific) #9502 (下)进行western blot分析。

Western blot analysis of extracts from SKW6.4 cells, untreated or anti-Fas-treated (1 µg/ml), and Jurkat cells, untreated or etoposide-treated (25 µM), using Caspase-8 (1C12) Mouse mAb #9746.

未处理或anti-Fas处理(1 µg/ml)的SKW6.4细胞的细胞提取物，以及未处理或etoposide (25 µM)处理的Jurkat细胞的细胞提取物，使用Caspase-8 (1C12) Mouse mAb 鼠单抗#9746进行western blot分析。

Western blot analysis of extracts from Jurkat cells, untreated or etoposide-treated (5 hr, 25μM), using Caspase-2 (C2) Mouse mAb #2224.

未处理或etoposide处理(5 hr, 25μM)的Jurkat细胞的细胞提取物，使用Caspase-2 (C2) Mouse mAb 鼠单抗#2224进行western blot分析。