| 货号 | 3860T |
| 描述 | PLCγ Antibody Sampler Kit provides an economical means of analyzing phospho and total PLCγ levels. PLCγ Antibody Sampler Kit contains enough primary and secondary antibodies to perform four western blot experiments with each antibody. |
| 目标/特异性 | Each antibody in the PLCγ Antibody Sampler Kit detects endogenous levels of its target protein. The antibodies do not cross react with other PLCs. |
| 供应商 | CST |
| 背景 | Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors, and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ, and PLCε. Phosphorylation is one of the key mechanisms that regulate the activity of PLC. PLCγ is activated by both receptor and non-receptor tyrosine kinases (2). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783, and 1245 (3). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (4). PLCγ2 is engaged in antigen-dependent signaling in B cells and collagen-dependent signaling in platelets. Phosphorylation by Btk or Lck at Tyr753, 759, 1197, and 1217 is correlated with PLCγ2 activity (5,6). |
| 存放说明 | -20C |
| 参考文献 | Singer, W.D. et al. (1997) Annu Rev Biochem 66, 475-509. Margolis, B. et al. (1989) Cell 57, 1101-7. Kim, H.K. et al. (1991) Cell 65, 435-41. Wang, Z. et al. (1998) Mol Cell Biol 18, 590-7. Watanabe, D. et al. (2001) J Biol Chem 276, 38595-601. Ozdener, F. et al. (2002) Mol Pharmacol 62, 672-9. |
Western blot analysis of extracts from various cell lines using PLCγ1 (D9H10) XP® Rabbit mAb #5690.对多种细胞系使用PLCγ1 (D9H10) XP?兔单克隆抗体#5690进行Western blot分析。 | |
Western blot analysis of extracts from 3T3 cells, untreated or treated with PDGF alone or PDGF and CIP/λ phosphatases, using Phospho-PLCγ1 (Tyr783) Antibody #2821 (upper) or PLCγ1 Antibody #2822 (lower).对3T3细胞抽提液，未处理或PDGF单独刺激或PDGF和CIP/λ磷酸酶处理，使用Phospho-PLCγ1 (Tyr783)抗体#2821（上图）或PLCγ1抗体#2822（下图）进行Western blot分析。 | |
Western blot analysis of extracts from HeLa cells, either mock-transfected or transfected for 48 hours with SignalSilence™ PLCγ1 siRNA I #6293 or siRNA II #6254, using PLCγ1 (D9H10) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcioma using PLCγ1 (D9H10) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PLCγ1 (D9H10) XP® Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using PLCγ1 (D9H10) XP® Rabbit mAb. | |
Western blot analysis of extracts from NIH/3T3 (lanes 1, 3, 5) and Ramos (lanes 2, 4, 6) cells, using PLCγ1 Antibody #2822 (lanes 1 and 2), PLCγ2 Antibody (lanes 5 and 6) or both antibodies (lanes 3 and 4). Results show that PLCγ2 Antibody is specific to the 150 kDa PLCγ2 band detected in Ramos cells, while PLCγ1 Antibody #2822 is specifc to the 160 kDa PLCγ1 band detected in both Ramos and NIH/3T3 cells.对NIH/3T3（列1，3，5）和Ramos细胞（列2，4，6）抽提液使用PLCγ1抗体#2822（列1，2）、PLCγ2抗体（列5，6）或两种抗体均用（列3，4）进行Western blot分析。结果显示PLCγ2抗体特异性的对Ramos细胞150 kDa PLCγ2条带，PLCγ1 抗体#2822特异性的对Ramos和NIH/3T3细胞160 kDa PLCγ1条带。 | |
Western blot analysis of extracts from Ramos cells, untreated or treated with anti-human IgM, using Phospho-PLCγ2 (Tyr1217) Antibody #3871 (upper), Phospho-PLCγ2 (Tyr 759) Antibody #3874 (middle), or PLCγ2 Antibody #3872 (lower).对Ramos细胞，未处理，或抗人IgM处理，使用Phospho-PLCγ2 (Tyr1217)抗体#3871（上图）、Phospho-PLCγ2 (Tyr 759) 抗体#3874（中图）或PLCγ2抗体#3872（下图）进行Western blot分析。 | |
Western blot analysis of extracts from untreated or anti-human IgM treated Ramos cells or lamda phosphatase-treated Ramos cell lysates, using Phospho-PLCgamma2 (Tyr759) Antibody (upper) or PLCgamma2 Antibody #3872 (lower). | |
Flow cytometric analysis of NIH3T3 cells, untreated (blue) or PDGF treated (green), using Phospho-PLCγ1 (Tyr783) Antibody. | |
Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-stimulated for the indicated times, using Phospho-PLCγ1 (Tyr783) Antibody (upper) or PLCγ1 Antibody #2822 (lower). | |
Western blot analysis of extracts from Ramos (lane 1), THP1 (lane 2), U-937 (lane 3) cells and mouse spleenocytes (lane 4), using PLCγ2 Antibody. | |
Western blot analysis of extracts from Ramos cells, untreated or treated with anti-human IgM, using Phospho-PLCgamma2 (Tyr1217) Antibody (upper) or PLCgamma2 Antibody #3872 (lower). | |
Western blot analysis of extracts from NIH/3T3 (lanes 1, 3, 5) and Ramos (lanes 2, 4, 6) cells, using PLCγ1 Antibody #2822 (lanes 1 and 2), PLCgamma2 Antibody (lanes 5 and 6) or both antibodies (lanes 3 and 4). Results show that PLCγ2 Antibody is specific to the 150 kDa PLCγ2 band detected in Ramos cells, while PLCγ1 Antibody #2822 is specifc to the 160 kDa PLCγ1 band detected in both Ramos and NIH/3T3 cells. | |
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition. |
