| 货号 | 4487T |
| 描述 | The RNAi Machinery Antibody Sampler Kit provides an economical means to analyze proteins associated with endogenous RNA interference. The kit contains enough primary and secondary antibodies to perform four western blot experiments. |
| 目标/特异性 | Each antibody in the RNAi Machinery Antibody Sampler Kit recognizes endogenous levels of their target protein and does not cross-react with other proteins. |
| 供应商 | CST |
| 背景 | RNA interference (RNAi) serves as a global mechanism of gene regulation in eukaryotes. Through interactions with Dicer, Drosha, Argonaute 2 (Ago2) and Miwi/Mili proteins, microRNA (miRNA) is processed within the nucleus and utilized for gene silencing and down regulation of gene expression. Dicer is a member of the RNase III family that specifically cleaves double-stranded RNA to generate microRNA (miRNA) (1). Long, primary transcripts (pri-miRNAs) are processed to stem-looped pre-miRNAs by the nuclear RNase III Drosha (2) and are then transported to the cytoplasm for further processing by Dicer to produce mature, 22-nucleotide miRNAs (3). The mature miRNA then becomes a part of the RNA-Induced Silencing Complex (RISC) and can bind to the 3 UTR of the target mRNA (3). Interference of Drosha pri-miRNA processing results in the increase of pri-miRNAs and the decrease of pre-miRNAs (2). Drosha forms part of a multiprotein complex called the Microprocessor along with other components, such as DGCR8 (4). Both Drosha and DGCR8 are necessary for miRNA biogenesis (5). Argonaute protein family members participate in various steps of miRNA-mediated gene silencing such as repression of translation and mRNA turnover (6). The Drosophila piwi gene was identified as being required for the self-renewal of germ-line stem cells, and its homologues are well conserved among various species including Arabidopsis, C. elegans and human (7). Miwi and Mili proteins are both mouse homologs of Piwi and contain a carboxy-terminal Piwi domain that binds to Piwi-interacting RNAs (piRNAs) in male germ cells and are essential for spermatogenesis in mouse (8-11). |
| 存放说明 | -20C |
| 参考文献 | 1 . Hutvágner, G. and Zamore, P.D. (2002) Science 297, 2056-60. 2 . Lee, Y. et al. (2003) Nature 425, 415-9. 3 . Peters, L. and Meister, G. (2007) Mol Cell 26, 611-23. 4 . Cox, D.N. et al. (1998) Genes Dev 12, 3715-27. 5 . Denli, A.M. et al. (2004) Nature 432, 231-5. 6 . Kuramochi-Miyagawa, S. et al. (2001) Mech Dev 108, 121-33. 7 . Diederichs, S. and Haber, D.A. (2007) Cell 131, 1097-108. 8 . Gregory, R.I. et al. (2004) Nature 432, 235-40. 9 . Aravin, A. et al. (2006) Nature 442, 203-7. 10 . Grivna, S.T. et al. (2006) Proc Natl Acad Sci USA 103, 13415-20. 11 . Grivna, S.T. et al. (2006) Genes Dev 20, 1709-14. |
Western blot analysis of extracts from various cell types using Argonaute 1 (D84G10) XP® Rabbit mAb #5053. 使用Argonaute 1 (D84G10) XP® Rabbit mAb兔单抗 #5053，免疫印迹（Western Blot）分析在不同细胞系提取物。 | |
Western blot analysis of extracts from MCF7 and 293 cells using Dicer (D38E7) Rabbit mAb #5362. 使用Dicer (D38E7) Rabbit mAb兔单抗 #5362，免疫印迹（Western Blot）分析在MCF7和293细胞系提取物。 | |
Confocal immunofluorescent analysis of mouse testis using Mili (D14F5) XP® Rabbit mAb (green) and Pan-Keratin (C11) Mouse mAb #4545 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from mouse testis and mouse brain using Mili (D14F5) XP® Rabbit mAb #5940 (upper) or β-Actin Antibody #4967 (lower). 使用Mili (D14F5) XP® Rabbit mAb兔单抗 #5940 (上图) 和β-Actin Antibody #4967 (下图)，免疫印迹（Western Blot）分析在不同细胞系提取物。 | |
Western blot analysis of extracts from various cell types using Argonaute 1 (D84G10) XP® Rabbit mAb. | |
Western blot analysis of extracts from mouse testis and mouse brain using Mili (D14F5) XP® Rabbit mAb (upper) or β-Actin Antibody #4967 (lower). | |
Immunohistochemical analysis of paraffin-embedded mouse testis using Mili (D14F5) XP® Rabbit mAb. | |
Western blot analysis of extracts from MCF7 and 293 cells using Dicer (D38E7) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human breast using Argonaute 1 (D84G10) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Western blot analysis of extracts from various cell lines using Argonaute 2 (C34C6) Rabbit mAb #2897. 使用Argonaute 2 (C34C6) Rabbit mAb 兔单抗#2897，免疫印迹（Western Blot）分析在不同细胞系提取物。 | |
Western blot analysis of extracts from various cell types using Argonaute 2 (C34C6) Rabbit mAb. | |
Western blot analysis of extracts from various cell types using Drosha (D28B1) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using Drosha (D28B1) Rabbit mAb #3364. 使用Drosha (D28B1) Rabbit mAb兔单抗 #3364，免疫印迹（Western Blot）分析在不同细胞系的提取物。 | |
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition. |
