| 货号 | 4638T |
| 目标/特异性 | Both total TrkA and TrkB antibodies detect endogenous levels of their respective Trk receptors and do not cross-react with related proteins. The Trk (pan) (C17F1) Rabbit mAb detects endogenous levels of total TrkA, TrkB, and TrkC proteins. Phospho-TrkA (Tyr490)/TrkB (Tyr516) (C35G9) Rabbit mAb detects endogenous levels of TrkA and TrkB only when phosphorylated at the indicated sites; this antibody may cross-react with Bcr-Abl phosphorylated at an unknown tyrosine residue. Phospho-TrkA (Tyr674/675)/TrkB (Tyr706/707) (C50F3) Rabbit mAb detects endogenous levels of TrkA and TrkB only when phosphorylated at the indicated sites; this antibody may cross-react with a protein of ~150 kDa phosphorylated at an unknown tyrosine residue. |
| 供应商 | CST |
| 背景 | The family of Trk receptor tyrosine kinases consists of TrkA, TrkB and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3. TrkA regulates proliferation and is important for development and maturation of the nervous system (1). Phosphorylation at Tyr490 is required for Shc association and activation of the Ras-MAP kinase cascade. Residues Tyr674/675 lie within the catalytic domain, and phosphorylation at this site reflects TrkA kinase activity (2-6). Point mutations, deletions and chromosomal rearrangements (chimeras) cause ligand-independent receptor dimerization and activation of TrkA. Many malignancies including breast, colon, prostate and thyroid carcinomas and acute myeloid leukemia have activated TrkA. Expression of TrkA in neuroblastomas is a good prognostic marker because it signals growth arrest and differentiation of cells originating from the neural crest (1).Phosphorylation sites are generally conserved between TrkA and TrkB receptors: Tyr490 of TrkA corresponds to Tyr512 in TrkB, and Tyr674/675 of TrkA to Tyr706/707 in TrkB of the human sequence (7). TrkB is overexpressed in tumors such as neuroblastoma, prostate adenocarcinoma and pancreatic ductal adenocarcinoma. Overexpression of TrkB in neuroblastomas correlates with unfavorable disease outcome when autocrine loops that signal tumor survival are potentiated by additional overexpression of brain-derived neurotrophic factor (BDNF). An alternatively spliced, truncated TrkB isoform that lacks the kinase domain is overexpressed in Wilms’s tumors; this isoform may act as a dominant-negative to TrkB signaling (8). |
| 存放说明 | -20C |
| 参考文献 | Huang, E.J. and Reichardt, L.F. (2003) Annu Rev Biochem 72, 609-42. Segal, R.A. and Greenberg, M.E. (1996) Annu Rev Neurosci 19, 463-89. Stephens, R.M. et al. (1994) Neuron 12, 691-705. Marsh, H.N. et al. (2003) J Cell Biol 163, 999-1010. Obermeier, A. et al. (1993) EMBO J 12, 933-41. Obermeier, A. et al. (1994) EMBO J 13, 1585-90. Arevalo, J.C. et al. (2001) Oncogene 20, 1229-34. Reuther, G.W. et al. (2000) Mol Cell Biol 20, 8655-66. Greco, A. et al. (1997) Genes Chromosomes Cancer 19, 112-23. Pierotti, M.A. and Greco, A. (2006) Cancer Lett 232, 90-8. Lagadec, C. et al. (2009) Oncogene 28, 1960-70. Greco, A. et al. (2010) Mol Cell Endocrinol 321, 44-9. Ødegaard, E. et al. (2007) Hum Pathol 38, 140-6. Huang, E.J. and Reichardt, L.F. (2003) Annu Rev Biochem 72, 609-42. Geiger, T.R. and Peeper, D.S. (2005) Cancer Res 65, 7033-6. Han, L. et al. (2007) Med Hypotheses 68, 407-9. Aoyama, M. et al. (2001) Cancer Lett 164, 51-60. Desmet, C.J. and Peeper, D.S. (2006) Cell Mol Life Sci 63, 755-9. |
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition. | |
Western blot analysis of extracts from NIH/3T3, NIH/3T3-TrkA, NIH/3T3-TrkB and NIH/3T3-TrkC and K562 cells using TrkA (14G6) Rabbit mAb (upper) TrkB (80G2) Rabbit mAb Antibody #4607 (middle) and PLCγ1 Antibody #2822 (lower). | |
Immunohistochemical analysis of paraffin-embedded human breast using TrkA (14G6) Rabbit mAb. | |
Western blot analysis of extracts from untreated or NGF-treated 3T3/TrkA and PC12 cells using Phospho-TrkA (Tyr490)/TrkB (Tyr516) (C35G9) Rabbit mAb. | |
Western blot analysis of extracts from NIH/3T3 cells stably transfected with either TrkA or TrkB, left untreated or treated with NGF or BDNF, respectively, using Phospho-TrkA (Tyr490)/TrkB (Tyr516) (C35G9) Rabbit mAb (upper) and pooled TrkA/TrkB antibodies (lower). | |
Western blot analysis of extracts from untreated or NGF-treated PC12 cells using Phospho-TrkA (Tyr674/675)/TrkB (Tyr706/707) (C50F3) Rabbit mAb. | |
Western blot analysis of extracts from NIH/3T3 cells stably transfected with TrkA or TrkB, and treated with NGF or BDNF, respectively, using Phospho-TrkA (Tyr674/675)/TrkB (Tyr706/707) (C50F3) Rabbit mAb (upper) and pooled TrkA/TrkB Antibodies (lower). | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Trk A (14G6) Rabbit mAb #2508 in the presence of control peptide (left) or Trk A Blocking Peptide #1435 (right). | |
Western blot analysis of extracts from neonatal mouse brain, rat brain and NIH/3T3/TrkB cells using TrkB (80E3) Rabbit mAb. | |
Confocal immunofluorescent analysis of NIH/3T3 cells, wild-type (upper left), NIH/3T3/TrkA (upper right), NIH/3T3/TrkB (lower left) or NIH/3T3/TrkC (lower right) using Trk (pan) (C17F1) Rabbit mAb (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). | |
Western blot analysis of extracts from fetal human brain and mouse brain using Trk (pan) (C17F1) Rabbit mAb. | |
Flow cytometric analysis of NIH/3T3 cells, untransfected (blue) or TrkA transfected (green), using Trk (pan) (C17F1) Rabbit mAb. | |
Western blot analysis of extracts from 3T3/TrkA and PC-12 cells, untreated or NGF-treated, using Phospho-TrkA (Tyr490)/TrkB (Tyr516) (C35G9) Rabbit mAb #4619.对未处理或NGF处理的3T3/TrkA和PC-12细胞抽提液使用Phospho-TrkA (Tyr490)/TrkB(Tyr516)(C35G9)兔单克隆抗体#4619进行Western blot分析。 | |
Western blot analysis of extracts from NIH/3T3 cells, stably transfected with either TrkA or TrkB and untreated or treated with NGF or BDNF, using Phospho-TrkA (Tyr490)/TrkB (Tyr516) (C35G9) Rabbit mAb #4619 (upper) and pooled TrkA/TrkB antibodies (lower).对稳定转染了TrkA或TrkB的NIH/3T3细胞，未处理或分别使用NGF或BDNF处理，然后使用Phospho-TrkA (Tyr490)/TrkB (Tyr516) (C35G9)兔单克隆抗体（上图）和合并TrkA/TrkB抗体（下图）进行Western blot分析。 | |
Western blot analysis of extracts from PC-12 cells, untreated or NGF-treated, using Phospho-TrkA (Tyr674/675)/TrkB (Tyr706/707) (C50F3) Rabbit mAb #4621.对未处理或NGF处理的PC12细胞抽提液使用Phospho-TrkA (Tyr674/675)/TrkB (Tyr706/707) (C50F3)兔单克隆抗体#4621进行Western blot分析。 |
