| 货号 | 4670T |
| 描述 | The Loading Control Antibody Sampler Kit (HRP Conjugate) contains antibodies directed against a variety of housekeeping proteins. These Cell Signaling Technology antibodies are conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The kit contains enough primary antibodies to perform four western blots. |
| 目标/特异性 | Each antibody in the Loading Control Antibody Sampler Kit (HRP Conjugate) detects endogenous levels of its target protein and does not typically cross-react with other proteins. Histone H3 (D1H2) XP® Rabbit mAb (HRP Conjugate) detects endogenous levels of total histone H3 protein, including the Histone H3 variant CENP-A. |
| 供应商 | CST |
| 标记 | HRP |
| 背景 | Housekeeping proteins perform numerous basic functions within the cell and are constitutively expressed at high levels in a variety of tissues and cell types. Western blot analysis commonly uses housekeeping proteins such as β-actin, COX IV, GAPDH, histone H3 and the α- and β-tubulins as loading controls. Actin is a ubiquitous protein and a major component of the eukaryotic cytoskeleton. Actin exists mainly as the F-actin fibrous polymer (1). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Recent work has demonstrated that GAPDH plays roles in apoptosis (2), gene expression (3), and nuclear transport (4). Globular tubulin subunits made up of α- and β-tubulin heterodimers are the building blocks of microtubules, one of three types of cytosolic fibers that comprise the cytoskeleton (5). Histone proteins, including histone H3, make up the primary building block of chromatin known as nucleosomes. Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes (6). Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (7-9). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of protons across the mitochondrial inner membrane to drive ATP synthesis (10). |
| 存放说明 | -20C |
| 参考文献 | 1 . Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79. 2 . Condeelis, J. (2001) Trends Cell Biol 11, 288-93. 3 . Hara, M.R. and Snyder, S.H. (2006) Cell Mol. Neurobiol. 26, 527-38. 4 . Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47. 5 . Capaldi, R.A. et al. (1983) Biochim. Biophys. Acta 726, 135-148. 6 . Zheng, L. et al. (2003) Cell 114, 255-66. 7 . Ostermeier, C. et al. (1996) Curr. Opin. Struct. Biol. 6, 460-466. 8 . Kadenbach, B. et al. (2000) Free Radic. Biol. Med. 29, 211-221. 9 . Bae, B.I. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 3405-9. 10 . Barrientos, A. et al. (2002) Gene 286, 53-63. |
Western blot analysis of extracts from various cell lines using β-Actin (D6A8) Rabbit mAb (HRP Conjugate). | |
Western blot analysis of extracts from various cell lines using Histone H3 (D1H2) XP® Rabbit mAb (HRP Conjugate). | |
Western blot analysis of extracts from NIH/3T3 and HeLa cells using GAPDH (D16H11) XP® Rabbit mAb (HRP Conjugate). | |
Western blot analysis of extracts from NIH/3T3 and HeLa cells using GAPDH (D16H11) XP® Rabbit mAb (HRP Conjugate) #8884. | |
Western blot analysis of extracts from various cell lines using β-Tubulin (9F3) Rabbit mAb (HRP Conjugate) #5346. | |
Western blot analysis of extracts from Jurkat, HeLa and COS cell lines, using COX IV (3E11) Rabbit mAb (HRP Conjugate). | |
Western blot analysis of extracts from Jurkat, HeLa and COS cells using COX IV (3E11) Rabbit mAb (HRP Conjugate) #5247. | |
Western blot analysis of extracts from various cell lines using β-Tubulin (9F3) Rabbit mAb (HRP Conjugate). |
