| 货号 | 5142T |
| 描述 | The Loading Control Antibody Sampler Kit contains antibodies to a variety of housekeeping proteins. The kit contains enough primary and secondary antibodies to perform four western blots per primary antibody. |
| 目标/特异性 | Each antibody in the Loading Control Antibody Sampler Kit detects endogenous levels of its target protein and does not typically cross-react with other proteins. |
| 供应商 | CST |
| 背景 | Housekeeping proteins perform numerous basic functions within the cell and are constitutively expressed at high levels in a variety of tissues and cell types. Western blot analysis commonly uses housekeeping proteins such as β-actin, COX IV, GAPDH, histone H3 and the α- and β-tubulins as loading controls. Actin is a ubiquitous protein and a major component of the eukaryotic cytoskeleton. Actin exists mainly as the F-actin fibrous polymer (1). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Recent work has demonstrated that GAPDH plays roles in apoptosis (2), gene expression (3), and nuclear transport (4). Globular tubulin subunits made up of α- and β-tubulin heterodimers are the building blocks of microtubules, one of three types of cytosolic fibers that comprise the cytoskeleton (5). Histone proteins, including histone H3, make up the primary building block of chromatin known as nucleosomes. Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes (6). Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (7-9). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of protons across the mitochondrial inner membrane to drive ATP synthesis (10). |
| 存放说明 | -20C |
| 参考文献 | 1 . Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79. 2 . Condeelis, J. (2001) Trends Cell Biol 11, 288-93. 3 . Hara, M.R. and Snyder, S.H. (2006) Cell Mol. Neurobiol. 26, 527-38. 4 . Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47. 5 . Capaldi, R.A. et al. (1983) Biochim. Biophys. Acta 726, 135-148. 6 . Zheng, L. et al. (2003) Cell 114, 255-66. 7 . Ostermeier, C. et al. (1996) Curr. Opin. Struct. Biol. 6, 460-466. 8 . Kadenbach, B. et al. (2000) Free Radic. Biol. Med. 29, 211-221. 9 . Bae, B.I. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 3405-9. 10 . Barrientos, A. et al. (2002) Gene 286, 53-63. |
Western blot analysis of extracts from various cell lines using β-Actin (D6A8) Rabbit mAb #8457. | |
Flow cytometric analysis of human peripheral blood lymphocytes using Histone H3 (D1H2) XP® Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody. | |
Flow cytometric analysis of NIH/3T3 cells using β-Actin (D6A8) Rabbit mAb (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype control #3900 (red). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody. | |
Immunohistochemical analysis of paraffin-embedded mouse colon using GAPDH (D16H11) XP® Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using β-Actin (D6A8) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using GAPDH (D16H11) XP® Rabbit mAb #5174. | |
Confocal immunofluorescent analysis of HeLa cells using β-Actin (D6A8) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Confocal immunofluorescent analysis of C2C12 cells using GAPDH (D16H11) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines using Histone H3 (D1H2) XP® Rabbit mAb #4499. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using GAPDH (D16H11) XP® Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using GAPDH (D16H11) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Histone H3 (D1H2) XP® Rabbit mAb. | |
Confocal immunofluorescent analysis of HeLa cells using Histone H3 (D1H2) XP® Rabbit mAb (green) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (red). | |
Western blot analysis of extracts from various cell lines using Histone H3 (D1H2) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using β-Tubulin (9F3) Rabbit mAb preincubated with control peptide (left) or β-Tubulin Blocking Peptide #1032 (right). |
