| 货号 | 8334T |
| 描述 | The Lipolysis Activation Antibody Sampler Kit provides an economical means to evaluate the activation status of multiple members of the lipolysis pathway, including phosphorylated HSL and perilipin. The kit includes enough antibody to perform four western mini-blot experiments with each primary antibody. |
| 目标/特异性 | Each antibody in the Lipolysis Activation Antibody Sampler Kit detects endogenous levels of the respective target protein. Phospho-HSL (Ser563) Antibody does not cross-react with Ser565 phosphorylated HSL. Phospho-HSL (Ser565) Antibody does not cross-react with Ser563 phosphorylated HSL. Phospho-HSL (Ser660) Antibody does not cross-react with Ser563, Ser565, or Ser659 phosphorylated HSL. |
| 供应商 | CST |
| 背景 | Triacylglycerol is stored in lipid droplets as a primary energy reserve. During lipolysis, triacylglycerols in adipocytes are hydrolyzed into free fatty acids and glycerol. Perilipin, localized at the periphery of lipid droplets, serves as a protective coating against lipases (1-3). Evidence suggests that PKA regulates lipolysis by phosphorylating perilipin and hormone-sensitive lipase (HSL) (1,2,4,5). Phosphorylation of perilipin results in the conformational change that exposes lipid droplets to endogenous lipases, such as HSL (2). Phosphorylation of HSL at Ser563, Ser659, and Ser660 by PKA stimulates HSL activity, which in turn catalyzes the hydrolysis of triacylglycerol (6,7).Application References |
| 存放说明 | -20C |
| 参考文献 | 1 . Degerman, E. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 533-37. 2 . Anthonsen, M.W. et al. (1998) J. Biol. Chem. 273, 215-21. 3 . Greenberg, A.S. et al. (1991) J Biol Chem 266, 11341-6. 4 . Brasaemle, D.L. (2007) J Lipid Res 48, 2547-59. 5 . Ducharme, N.A. and Bickel, P.E. (2008) Endocrinology 149, 942-9. 6 . Egan, J.J. et al. (1990) J Biol Chem 265, 18769-75. 7 . Brasaemle, D.L. et al. (2009) Mol Cell Biochem 326, 15-21. |
Western blot analysis of extracts from human pre-adipocytes and adipocytes using Perilipin (D1D8) XP® Rabbit mAb #9349 (upper) and β-Actin Antibody #4967 (lower). Wetern blot检测人前脂肪细胞和脂肪细胞提取物，所用抗体为 Perilipin (D1D8) XP® Rabbit mAb兔单抗 #9349 (上)和β-Actin Antibody #4967 (下)。 | |
Western blot analysis of extracts from various cell lines using HSL Antibody #4107.Wetern blot检测多种细胞系提取物，所用抗体为HSL Antibody #4107。 | |
Western blot analysis of extracts from differentiated 3T3-L1 cells treated with isoproterenol or λ-phosphatase, using Phospho-HSL (Ser563) Antibody #4139. Wetern blot检测分化的3T3-L1细胞提取物，细胞用异丙肾上腺素或λ磷酸酶处理。所用抗体为 Phospho-HSL (Ser563) Antibody #4139。 | |
Western blot analysis of extracts from differentiated 3T3-L1 cells treated with forskolin, oligomycin or λ-phosphatase, using Phospho-HSL (Ser565) Antibody #4137. Wetern blot检测分化的3T3-L1细胞提取物，细胞用毛喉素，寡霉素或λ磷酸酶处理，所用抗体为Phospho-HSL (Ser565) Antibody #4137 | |
Western blot analysis of extracts from differentiated NIH/3T3-L1 cells treated with isoproterenol, using Phospho-HSL (Ser660) Antibody #4126. Wetern blot检测分化的NIH/3T3-L1细胞系提取物，细胞用异丙肾上腺素处理，所用抗体为Phospho-HSL (Ser660) Antibody #4126 | |
Immunohistochemical analysis of paraffin-embedded breast carcinoma using Perilipin (D1D8) XP® Rabbit mAb. Note specific staining of adipocytes. | |
Immunohistochemical analysis of parafin-embedded mouse brown fat using Perilipin (D1D8) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Western blot analysis of extracts from human pre-adipocytes and adipocytes using Perilipin (D1D8) XP® Rabbit mAb (upper) and β-Actin Antibody #4967 (lower). | |
Confocal immunofluorescent analysis of frozen mouse brown adipose tissue using Perilipin (D1D8) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Confocal immunofluorescent analysis of 8-day differentiated 3T3-L1 cells, using Perilipin (D1D8) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Confocal immunofluorescent analysis of 3T3-L1 adipocytes using HSL Antibody (red). Lipid droplets have been labeled with BODIPY 493/503 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from NIH/3T3 and 3T3-L1 cells using HSL Antibody. | |
Confocal immunofluorescent analysis of 3T3-L1 adipocytes, isoproterenol-treated (left) or untreated (right), labeled with Phospho-HSL (Ser660) Antibody (red). Lipid droplets have been labeled with BODIPY 493/503 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Confocal immunofluorescent analysis of 3T3-L1 cells, untreated (left) or phosphatase-treated (right), labeled with Phospho-HSL (Ser565) Antibody (red) showing cytoplasmic localization in differentiated cells. Lipid droplets have been labeled with BODIPY® 493/503 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Confocal immunofluorescent analysis of 3T3-L1 adipocytes, isoproterenol-treated (left) or phosphatase-treated (right), labeled with Phospho-HSL (Ser563) Antibody (red). Lipid droplets have been labeled with BODIPY 493/503 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
