| 货号 | 8344T |
| 描述 | MRN Complex Antibody Sampler Kit offers an economical way of detecting each target protein. The kit contains enough primary and secondary antibody to perform four western blot experiments with each primary antibody.MRN Complex Antibody Sampler Kit能够经济地检测每个靶蛋白。该试剂盒内含足够4次western blot实验的一抗和二抗。 |
| 目标/特异性 | Antibodies detect endogenous levels of their respective proteins. |
| 供应商 | CST |
| 背景 | The Mre11-Rad50-Nbs1 (MRN) complex is a key mediator of genome maintenance, playing important roles in meiosis, telomere stability at the ends of chromosomes, and the cellular responses to DNA damage (1-5). Homodimers of the Mre11 and Rad50 subunits form a tetramer core that binds directly to DNA and associates with the Nbs1 subunit (6). The complex functions as a sensor of DNA damage and localizes to DNA double-strand breaks. At these DNA lesions, the MRN complex tethers DNA ends and processes free strands via the endonuclease and exonuclease activities of Mre11. In addition to stimulating both homologous recombination and nonhomologous end joining repair DNA pathways, MRN activates DNA damage checkpoint signaling cascades regulating cell cycle progression. In some contexts, MRN is required for ATM activation and downstream phosphorylation of p53, BRCA1, and Chk2 (7). ATM also phosphorylates Mre11, Rad50, and Nbs1 (also known as p95 and Nibrin). Notably, Nbs1 Ser343 and Mre11 Ser676 are phosphorylated by ATM. Phosphorylation modulates function and association with many mediators, some of which include 53BP1, RPA, hSSB1, TRF2, BRCA1, FANCD2, CtP1, Histone H2AX, MDC1, and WRN helicase. Each subunit is essential for mammalian embryonic development, as mice with homozygous-null mutations in Mre11, Nbs1, or Rad50 are lethal. Furthermore, MRN complex function is required in developing lymphocytes for antigen receptor gene recombination initiated by the Rag-1 and Rag-2 recombinases. In humans, Mre11 and Nbs1 mutations cause chromosomal instability and radiosensitivity and are associated with ataxia-telangiectasia-like disorder (ATLD) and Nijmegen breakage syndrome (NBS), respectively (8). Genomic instability and cancer have been shown to develop in cells with genetic mutations within MRN complex genes.Mre11-Rad50-Nbs1 (MRN)复合物是一个重要的基因组维护介质，在减数分裂时、染色体末端的端粒稳定和细胞应答DNA损伤过程中起着重要作用(1-5)。Mre11和Rad50亚基的同源二聚体形成的四聚体核心，直接结合DNA并与NBS1蛋白亚基联合(6)。该复合体作为DNA损伤传感器并定位于DNA双链断裂处。在这些DNA损伤中，MRN复合物拴住DNA末端，并通过Mre11的核酸内切酶和外切酶的活性处理自由链。除了刺激同源重组和非同源末端连接修复DNA途径以外，MRN激活DNA损伤检验点信号级联反应调节细胞周期进程。在某些情况下，MRN为ATM激活和下游p53、BRCA1和Chk2的磷酸化所必需(7)。ATM还可以磷酸化Mre11，Rad50和NBS1(也称为P95和Nibrin)。值得注意的是，NBS1 Ser343和MRE11 Ser676被ATM磷酸化。磷酸化调节蛋白功能和与许多介质的相关性，其中包括53BP1，RPA，hSSB1，TRF2，BRCA1，FANCD2，CTP1，组蛋白H2AX，MDC1和WRN解旋酶。每个亚基对哺乳动物的胚胎发育是必不可少的，作为纯合子无效突变中的Mre11，NBS1，或Rad50蛋白对小鼠是致命的。此外，MRN复合物的功能为淋巴细胞发育中RAG-1和RAG-2的重组酶启动的抗原受体基因重组所必需。在人类中，Mre11和NBS1基因突变导致染色体不稳定和辐射敏感性并分别与共济失调毛细血管扩张症状症(ATLD)和Nijmegen断裂综合征(NBS)相关(8)。已经证实具有MRN复合物基因内突变的细胞会发生基因组不稳定和癌症。Application References |
| 存放说明 | -20C |
| 参考文献 | 1 . DAmours, D. and Jackson, S.P. (2002) Nat Rev Mol Cell Biol 3, 317-27. 2 . van den Bosch, M. et al. (2003) EMBO Rep 4, 844-9. 3 . Ajimura, M. et al. (1993) Genetics 133, 51-66. 4 . Deng, Y. et al. (2009) Nature 460, 914-8. 5 . Lamarche, B.J. et al. (2010) FEBS Lett 584, 3682-95. 6 . Zhao, S. et al. (2000) Nature 405, 473-7. 7 . Williams, R.S. et al. (2009) Cell 139, 87-99. 8 . Uziel, T. et al. (2003) EMBO J 22, 5612-21. |
Immunoprecipitation of p95/NBS1 from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or p95/NBS1 (D6J5I) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using p95/NBS1 (D6J5I) Rabbit mAb. | |
Confocal immunofluorescent analysis of SK-MEL-28 (left) and GM07166 (right) cells using p95/NBS1 (D6J5I) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054. | |
Western blot analysis of extracts from various cell lines using p95/NBS1 (D6J5I) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). | |
Western blot analysis of extracts from Mv 1 Lu cells, treated with UV or hydroxyurea (HU) for the indicated times, using Phospho-p95/NBS1 (Ser343) Antibody #3001.Western blot 方法检测紫外或羟基脲处理不同时间的Mv 1 Lu 细胞，使用的抗体为Phospho-p95/NBS1 (Ser343) Antibody #3001。 | |
Western blot analysis of extracts from various cell lines using p95/NBS1 Antibody #3002.Western blot 方法检测不同细胞提取物，使用的抗体为p95/NBS1 Antibody #3002。 | |
Western blot analysis of extracts from Jurkat and K562 cells using Rad50 Antibody #3427.Western blot 方法检测Jurkat和K562细胞提取物，使用的抗体为Rad50 Antibody #3427。 | |
Western blot analysis of extracts from HeLa and HT-1080 cells, untreated or treated with UV, using Phospho-Mre11 (Ser676) Antibody #4859 (upper) or total Mre11 Antibody #4895 (lower).Western blot 检测未处理或紫外处理的HeLa 和 HT-1080细胞提取物，使用的抗体为 Phospho-Mre11 (Ser676) Antibody #4859 (上图) 或total Mre11 Antibody #4895 (下图). | |
Western blot analysis of extracts from HeLa and HT-1080 cells, untreated or treated with UV, using Phospho-Mre11 (Ser676) Antibody (upper) or total Mre11 Antibody #4895 (lower). | |
Western blot analysis of extracts from HeLa and K-562 cells using Mre11 (31H4) Rabbit mAb #4847.Western blot 方法检测Hela和k-562细胞提取物，使用的抗体为Mre11 (31H4) Rabbit mAb #4847。 | |
Immunohistochemical analysis of frozen SKOV-3 xenograft using Mre11 (31H4) Rabbit mAb. | |
Western blot analysis of extracts from Jurkat and K562 cells, using RAD50 Antibody. | |
Flow cytometric analysis of K562 cells, using Mre11 (31H4) Rb mAb (blue) compared to a nonspecific negative control antibody (red). | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Mre11 (31H4) Rabbit mAb in the presence of control peptide (left) or Mre11 Blocking Peptide #1035 (right). | |
Western blot analysis of extracts from COS, PC12, NIH3T3, HeLa and Mv1Lu cells, using p95/NBS1 Antibody. | |
Western blot analysis of extracts from HeLa and K562 cells, using Mre11 Rabbit (31H4) mAb. |
