| 货号 | 8358T |
| 描述 | This sampler kit provides an economical means of evaluating key members of the Hedgehog signaling pathway. The kit contains enough primary and secondary antibody to perform four western miniblot experiments. 此抗体样品试剂盒提供经济节省方式来检测Hedgehog信号通路蛋白。.这种试剂盒包含足够做四次小的western blot实验所需的一抗和二抗。 |
| 目标/特异性 | Shh (C9C5) Rabbit mAb detects endogenous levels of total Shh protein. This antibody does not cross-react with transfected IHH and DHH. PTCH1 (C53A3) Rabbit mAb detects transfected levels of PTCH1. This antibody can also detect endogenous levels of PTCH1 through immunoprecipitation followed by Western blot analysis. PTCH2 (G1191) Antibody detects transfected levels of PTCH2 protein. It does not recognize transfected levels of human PTCH1 protein. SUFU (C54G2) Rabbit mAb detects endogenous levels of total SUFU protein. GLI1 (V812) Antibody detects endogenous levels of total GLI1 protein. |
| 供应商 | CST |
| 背景 | The evolutionarily conserved Hedgehog (Hh) signaling pathway plays critical roles in the regulation of patterning, growth, and cell migration during embryonic development and adult tissue homeostasis. Aberrant Hh signaling activity can be associated with numerous birth defects and uncontrolled Hh pathway activation is linked to the development of several types of cancers (1-2). The three identified vertebrate Hh genes are Sonic (Shh), Indian (Ihh), and Desert (Dhh), all of which have distinct as well as overlapping roles (3-5). Patched1 and 2 (PTCH1 and PTCH2) are twelve-pass transmembrane proteins that function as the Hh receptors (6-9). The general organization of the Hh pathway consists of a series of repressive interactions. In the absence of Hh proteins (off-state), PTCH suppresses the otherwise constitutively active signaling receptor Smoothened (Smo) (1,2). In the off-state, SUFU (Suppressor of Fused), originally identified in Drosophila as a suppressor of the Fused (Fu) kinase (10), suppresses Hh signaling by regulating the localization of the transcription factors Gli and Ci (11,12). In Drosophila, SUFU may also positively regulate Hh signaling depending on SUFU protein levels and Hh signal intensity (13). |
| 存放说明 | -20C |
| 参考文献 | 1 . Stone, D.M. et al. (1996) Nature 384, 129-34. 2 . Pham, A. et al. (1995) Genetics 140, 587-98. 3 . Chen, Y. and Struhl, G. (1996) Cell 87, 553-63. 4 . Barnfield, P.C. et al. (2005) Differentiation 73, 397-405. 5 . Zhang, X.M. et al. (2001) Cell 106, 781-92. 6 . Motoyama, J. et al. (1998) Nat Genet 18, 104-6. 7 . Méthot, N. and Basler, K. (2000) Development 127, 4001-10. 8 . Ingham, P.W. and McMahon, A.P. (2001) Genes Dev 15, 3059-87. 9 . Adolphe, C. et al. (2004) Development 131, 5009-19. 10 . Smyth, I. et al. (1999) Hum Mol Genet 8, 291-7. 11 . Dussillol-Godar, F. et al. (2006) Dev Biol 291, 53-66. 12 . McMahon, A.P. et al. (2003) Curr Top Dev Biol 53, 1-114. 13 . Pathi, S. et al. (2001) Mech Dev 106, 107-17. |
Western blot analysis of extracts from RMS-13 and TOV-112D cells using GLI1 (V812) Antibody #2534.使用 GLI1 (V812) 抗体#2534对RMS-13和TOV-112D 细胞提取物进行western blot分析。 | |
Western blot analysis of total cell lysates from various cell types using SUFU (C54G2) Rabbit mAb #2520.使用SUFU (C54G2) 兔单抗#2520对多种细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from COS cells, untransfected or transiently transfected with a construct expressing human PTCH2, using PTCH2 (G1191) Antibody #2470.使用PTCH2 (G1191)抗体#2470对未转染或瞬转人PTCH2表达质粒的COS细胞提取物进行western blot分析。 | |
Western blot analysis of total cell lysates from COS cells, untransfected or transiently transfected with a human PTCH1 construct, using PTCH1 (C53A3) Rabbit mAb #2468.未转染或瞬转人PTCH1表达质粒的COS细胞，取得细胞提取物，使用PTCH1 (C53A3)兔单抗进行western blot分析。 | |
Western blot analysis of extracts from HT29 and GH3 cells, and GH3 cell conditioned medium (CM), using Shh (C9C5) Rabbit mAb #2207.使用Shh (C9C5) 兔单抗#2207对HT29和GH3细胞及GH3细胞培养基（CM）提取物进行western blot分析。 | |
Western blot analysis of extracts from RMS-13 and TOV-112D cells using GLI1 (C68H3) Rabbit mAb. | |
Western blot analysis of extracts from HT29 and GH3 cells, and GH3 cell conditioned medium (CM), using Shh (C9C5) Rabbit mAb. | |
Immunoprecipitation of endogenous PTCH1 from 293 cell lysates using PTCH1 (C53A3) Rabbit mAb. Western blot detection was performed using the same antibody. | |
Western blot analysis of total cell lysates from various cell types using SUFU (C54G2) Rabbit mAb. | |
Western blot analysis of total cell lysates from COS cells, untransfected or transiently transfected with a human PTCH1 expression construct, using PTCH1 (C53A3) Rabbit mAb. | |
Western blot analysis of extracts from COS cells, untransfected or transiently transfected with a construct expressing human PTCH2, using PTCH2 (G1191) Antibody. | |
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition. |
