| 货号 | 8648T |
| 描述 | The Parkinsons Research Antibody Sampler Kit provides an economical means of detecting target proteins related to Parkinsons disease. The kit contains enough primary and secondary antibody to perform four western blots per primary. |
| 目标/特异性 | DJ-1 (D29E5) XP® Rabbit mAb, LRRK2 Antibody, Parkin (Prk8) Mouse mAb, and PINK1 (D8G3) Rabbit mAb recognize endogenous levels of respective target proteins. α-Synuclein (D37A6) XP® Rabbit mAb recognizes endogenous levels of the α isoform of synuclein protein. |
| 供应商 | CST |
| 背景 | Parkinson’s disease (PD), the second most common neurodegenerative disease after Alzheimer’s, is a progressive movement disorder characterized by rigidity, tremors, and postural instability. The pathological hallmark of PD is progressive loss of dopaminergic neurons in the substantia nigra of the ventral midbrain and the presence of intracellular Lewy bodies in surviving neurons of the brain stem (1). Research studies have shown that various genes and loci (α-synuclein/PARK1 and 4, parkin/PARK2, UCH-L1/PARK5, PINK1/PARK6, DJ-1/PARK7, LRRK2/PARK8, synphilin-1, and NR4A2) are genetically linked to PD (2). α-Synuclein, a 140 amino acid protein expressed abundantly in the brain, is a major component of aggregates found in Lewy bodies (3). Parkin is involved in protein degradation through the ubiquitin-proteasome pathway, and investigators have shown that mutations in Parkin cause early onset of PD (4). In the case of autosomal recessive juvenile Parkinsonism (AR-JP), deletions have been found on chromosome 6 in the Parkin gene (5). PTEN induced putative kinase 1 (PINK1) is a mitochondrial serine/threonine kinase involved in the normal function and integrity of mitochondria, as well as a reduction of cytochrome c release from mitochondria (6-8). PINK1 phosphorylates Parkin and promotes its translocation to mitochondria (7). Mutations of PINK1 are associated with loss of protective function, mitrochondrial dysfunction, aggregation of α-synuclein, and proteasome dysfunction (6,8). DJ-1 is involved in multiple cellular functions; it has been shown to cooperate with Ras to increase cell transformation, to regulate transcription of the androgen receptor, and may function as an indicator of oxidative stress, while loss-of-function mutations in DJ-1 cause early onset of PD (9-12). Dopamine D2 receptor-mediated functions are greatly impaired in DJ-1 (-/-) mice, resulting in reduced long-term depression (13). Leucine-rich repeat kinase 2 (LRRK2) contains amino-terminal leucine-rich repeats (LRR), a Ras-like small GTP binding protein-like (ROC) domain, an MLK protein kinase domain, and a carboxy-terminal WD40-repeat. At least 20 LRRK2 mutations have been linked to PD (14). The most prevalent mutation, G2019S, causes increased LRRK2 kinase activity, leading to progressive neurite loss and decreased neuronal survival (15). |
| 存放说明 | -20C |
| 参考文献 | 1 . Fahn, S. (2003) Ann. NY Acad. Sci. 991, 1-14. 2 . Goldberg, M.S. and Lansbury Jr., P.T. (2000) Nat. Cell Biol. 2, 115-119. 3 . Borrelli, E. (2005) Neuron 45, 479-81. 4 . Liu, W. et al. (2009) PLoS One 4, e4597. 5 . Moore, D.J. et al. (2005) Annu. Rev. Neurosci. 28, 57-87. 6 . Kim, Y. et al. (2008) Biochem Biophys Res Commun 377, 975-80. 7 . Bonifati, V. et al. (2003) Science 299, 256-9. 8 . Petit, A. et al. (2005) J Biol Chem 280, 34025-32. 9 . Nagakubo, D. et al. (1997) Biochem. Biophys. Res. Commun. 231, 509-13. 10 . Mata, I.F. et al. (2006) Trends Neurosci. 29, 286-293. 11 . Takahashi, K. et al. (2001) J. Biol. Chem. 276, 37556-63. 12 . MacLeod, D. et al. (2006) Neuron 52, 587-593. 13 . Polymeropoulos, M.H. et al. (1997) Science 276, 2045-7. 14 . Mitsumoto, A. and Nakagawa, Y. (2001) Free Radic. Res. 35, 885-93. 15 . Goldberg, M.S. et al. (2005) Neuron 45, 489-96. |
Western blot analysis of extracts from mouse and rat brain using α-Synuclein (D37A6) XP® Rabbit mAb #4179. Western blot 分析小鼠和大鼠大脑提取物，使用的抗体是α-Synuclein (D37A6) XP® Rabbit mAb兔单抗 #4179。 | |
Western blot 分析小鼠LRRK2-/-突变型和野生型组织，使用的抗体是LRRK2 Antibody兔多抗 #5559（上图）或TrkB (80E3) Rabbit mAb兔单抗 #4603（下图）。（小鼠野生型和LRRK2-/-突变型大脑是由麻萨诸塞州波士顿市布里格姆妇产医院及哈佛医学院Jie Shen博士友情赠送。） | |
Western blot analysis of extracts from mouse LRRK2-/- and wild-type tissue using LRRK2 Antibody #5559 (upper) or TrkB (80E3) Rabbit mAb #4603 (lower). (Mouse wild-type and LRRK2-/- brains were kindly provided by Dr. Jie Shen, Brigham and Womens Hospital and Harvard Medical School, Boston, MA). | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a cDNA construct expressing full-length human PINK1 (hPINK1, +) using PINK1 (D8G3) Rabbit mAb. Western blot 分析293T细胞、模拟转染（-）或转染了表达全长人PINK1(hPINK1, +)的cDNA载体的细胞，使用的抗体是PINK1 (D8G3) Rabbit mAb兔单抗。 | |
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with CCCP (10 μM, 24 hr; +), using PINK1 (D8G3) Rabbit mAb. Western blot 分析 HeLa细胞提取物，未处理 (-)或经CCCP (10 μM, 24 hr; +)处理，使用的抗体为PINK1 (D8G3) Rabbit mAb兔单抗 。 | |
Western blot analysis of extracts from MEF wild-type, MEF DJ-1 (-/-), HeLa, and C6 cells using DJ-1 (D29E5) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). (MEF wild-type and MEF DJ-1 (-/-) cells were kindly provided by Dr. Philipp Kahle, University of Tübingen, Germany). Western blot 分析MEF野生型、MEF DJ-1 (-/-)突变型、HeLa和C6 细胞系提取物，使用的抗体为DJ-1 (D29E5) XP® Rabbit mAb兔单抗（上图）和β-Actin (D6A8) Rabbit mAb 兔单抗 #8457 （下图）。（MEF野生型和MEF DJ-1 (-/-)突变型细胞由德国蒂宾根大学的Philipp Kahle博士友情赠送。） | |
共聚焦免疫荧光分析MEF野生型（左）或MEF DJ-1(-/-)突变型(右)细胞系，使用的抗体是DJ-1 (D29E5) XP® Rabbit mAb 兔单抗(绿色)。肌动蛋白微丝用DY-554 phalloidin (红色)标记。蓝色伪彩=DRAQ5® #4084 (荧光DNA染料） (MEF野生型和MEF DJ-1 (-/-)突变型细胞由德国蒂宾根大学的Philipp Kahle博士友情赠送。） | |
Confocal immunofluorescent analysis of MEF wild-type (left) or MEF DJ-1 (-/-) (right) cells using DJ-1 (D29E5) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). (MEF wild-type and MEF DJ-1 (-/-) cells were kindly provided by Dr. Philipp Kahle, University of Tübingen, Germany). | |
Confocal immunofluorescent analysis of normal rat cerebellum, hippocampus and striatum using α-Synuclein (D37A6) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析正常大鼠小脑、海马和纹状体，使用的抗体是α-Synuclein (D37A6) XP® Rabbit mAb兔单抗 (绿色)。蓝色伪彩=DRAQ5® #4084 (荧光DNA染料）。 | |
Immunohistochemical analysis of paraffin-embedded mouse brain using α-Synuclein (D37A6) XP® Rabbit mAb. 免疫组化分析石蜡包埋的小鼠大脑切片，使用的抗体是α-Synuclein (D37A6) XP® Rabbit mAb兔单抗。 | |
Western blot analysis of extracts from mouse and rat brain using α-Synuclein (D37A6) XP® Rabbit mAb. Western blot分析小鼠和大鼠的脑提取物，使用的抗体是 α-Synuclein (D37A6) XP® Rabbit mAb兔单抗。 | |
Western blot analysis of extracts from PC12 cells, fetal rat brain and mouse brain, using Parkin (Prk8) Mouse mAb. Western blot分析PC12细胞、大鼠胚胎的脑和小鼠的脑提取物，使用的抗体是 Parkin (Prk8) Mouse mAb鼠单抗。 | |
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition. 一抗与目标蛋白结合后，与HRP偶联二抗的复合体形成。然后 LumiGLO*加入，酶催化分解后发光。 |
