| 货号 | 8653T |
| 描述 | The Organelle Localization IF Antibody Sampler Kit provides an economical means for identification of cellular organelles by fluorescence immunocytochemistry (IF-IC). This kit includes enough primary antibody to perform at least twenty IF-IC tests or four western blots with each antibody. |
| 目标/特异性 | Each antibody in the Organelle Localization IF Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members. Each antibody has been validated for IF-IC and stains the organelles indicated above. Expression of these proteins may vary in different cells and tissues. Please see www.cellsignal.com for additional specificity/sensitivity information for individual kit components. |
| 供应商 | CST |
| 背景 | Knowledge of the subcellular location of a protein may reveal its potential role in a variety of cellular processes. One can confirm the subcellular location of a marker that colocalizes with one of the organelle-specific antibodies in this kit. While these antibodies serve as powerful tools for immunofluorescence, they may also be used as western blot controls for fractionated cell lysates. |
| 存放说明 | -20C |
Immunohistochemical analysis of human ovarian serous cystadenoma using LAMP1 (D2D11) XP(R) Rabbit mAb. | |
Immunohistochemical analysis of normal human kidney using LAMP1 (D2D11) XP(R) Rabbit mAb. | |
Immunohistochemical analysis of human lung carcinoma using LAMP1 (D2D11) XP(R) Rabbit mAb. | |
Confocal immunofluorescent analysis of HeLa cells using LAMP1 (D2D11) XP® Rabbit mAb #9091 (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析HeLa细胞，使用的抗体是LAMP1 (D2D11) XP® Rabbit mAb #9091（绿色）。肌动蛋白丝用DY-554鬼笔环肽标记（红色）。蓝色假色= DRAQ5® #4084（DNA荧光染料）。 | |
Confocal immunofluorescent analysis of HeLa cells using EEA1 (C45B10) Rabbit mAb #3288 (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析HeLa细胞，使用的抗体是EEA1 (C45B10) Rabbit mAb #3288（绿色）。肌动蛋白丝用DY-554鬼笔环肽标记（红色）。蓝色假色= DRAQ5® #4084（DNA荧光染料）。 | |
Confocal immunofluorescent analysis of HeLa cells using AIF (D39D2) XP® Rabbit mAb #5318 (green), showing colocalization with mitochondria that were labeled with MitoTracker® Red CMXRos #9082 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析HeLa细胞，使用的抗体是AIF (D39D2) XP® Rabbit mAb #5318（绿色），结果显示其与MitoTracker® Red CMXRos #9082标记的线粒体处于同一位置（红色）。蓝色假色= DRAQ5® #4084（DNA荧光染料）。 | |
Confocal immunofluorescent analysis of NIH/3T3 cells using PDI (C81H6) Rabbit mAb #3501 (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析NIH/3T3细胞，使用的抗体是：PDI (C81H6) Rabbit mAb #3501（绿色）、β-Actin (8H10D10) Mouse mAb #3700（红色）。蓝色假色= DRAQ5® #4084（DNA荧光染料）。 | |
Confocal immunofluorescent analysis of MCF7 cells, untreated (upper) or treated with Brefeldin A #9972 (5 μg/ml, 1 hr; lower), using RCAS1 (D2B6N) XP® Rabbit mAb #12290 (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析MCF7细胞，未处理（上图）或用Brefeldin A #9972 (5 μg/ml, 1小时;下图)处理，使用的抗体是：RCAS1 (D2B6N) XP® Rabbit mAb #12290（绿色）。肌动蛋白丝用DY-554鬼笔环肽标记（红色）。蓝色假色= DRAQ5® #4084（DNA荧光染料）。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human RCAS1 (hRCAS1-Myc/DDK; +), using RCAS1 (D2B6N) XP®Rabbit mAb. | |
Immunoprecipitation of RCAS1 from HCT 116 extracts using Rabbit (DA1E) mAb IgG XP®Isotype Control #3900 (lane 2) or RCAS1 (D2B6N) XP®Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using RCAS1 (D2B6N) XP®Rabbit mAb. | |
Confocal immunofluorescent analysis of MCF7 cells, untreated (left) or treated with Brefeldin A #9972 (5 μg/ml, 1 hr; right), using RCAS1 (D2B6N) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines using RCAS1 (D2B6N) XP®Rabbit mAb. | |
Confocal immunofluorescent analysis of C2C12 cells using AIF (D39D2) XP® Rabbit mAb #5318 (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Flow cytometric analysis of Jurkat cells using RCAS1 (D2B6N) XP® Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP®Isotype Control #3900 (red). | |
Western blot analysis of extracts from various cell lines using LAMP1 (D2D11) XP® Rabbit mAb. |
