| 货号 | 9652T |
| 描述 | The PTEN and PDK1 Sampler Kit provides an economical means to evaluate two key enzymes that regulate multiple signaling pathways. The kit contains enough primary and secondary antibodies to perform four Western blots per primary antibody. |
| 目标/特异性 | Each antibody in the PTEN and PDK1 Antibody Sampler Kit detects endogenous levels of its target protein. Activation state antibodies detect only target proteins phosphorylated at indicated residues. Non-Phospho-PTEN (Ser380/Thr382/Thr383) Antibody detects endogenous levels of PTEN only when dephosphorylated at Ser380, Thr382 and Thr383. |
| 供应商 | CST |
| 背景 | PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein levels and activity (8) and is involved in G protein-coupled signaling during chemotaxis (9,10). |
| 存放说明 | -20C |
| 参考文献 | Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5. Myers, M.P. et al. (1997) Proc Natl Acad Sci USA 94, 9052-7. Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8. Wan, X. and Helman, L.J. (2003) Oncogene 22, 8205-11. Wu, X. et al. (1998) Proc Natl Acad Sci USA 95, 15587-91. Vazquez, F. et al. (2000) Mol Cell Biol 20, 5010-8. Torres, J. and Pulido, R. (2001) J Biol Chem 276, 993-8. Freeman, D.J. et al. (2003) Cancer Cell 3, 117-30. Funamoto, S. et al. (2002) Cell 109, 611-23. Iijima, M. and Devreotes, P. (2002) Cell 109, 599-610. Belham, C. et al. (1999) Curr Biol 9, R93-6. Toker, A. and Newton, A.C. (2000) Cell 103, 185-8. Williams, M.R. et al. (2000) Curr Biol 10, 439-48. |
Western blot analysis of extracts from various cell lines using Non-phospho PTEN (Ser380/Thr382/Thr383) (D2D11) Rabbit mAb #7960. The non-phosphospecificity of the antibody was verified by preincubating the antibody without peptide (-), with PTEN (Ser380/Thr382/Thr383) non-phosphopeptide (+), or with PTEN (Ser380/Thr382/Thr383) phosphopeptide (+) prior to incubating the membrane. | |
Western blot analysis of extracts from various cell lines using Non-phospho PTEN (Ser380/Thr382/Thr383) (D2D11) Rabbit mAb. The non-phosphospecificity of the antibody was verified by preincubating the antibody without peptide (-), with PTEN (Ser380/Thr382/Thr383) non-phosphopeptide (+), or with PTEN (Ser380/Thr382/Thr383) phosphopeptide (+) prior to incubating the membrane. | |
Western blot analysis of extracts from PC3 cells, HCT116 wild-type and HCT116 PDK1 -/- cells using Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower). (HCT116 wild-type and HCT116 PDK1 -/- cells were kindly provided by Dr. Bert Vogelstein, Johns Hopkins University, Baltimore, MD). | |
Western blot analysis of extracts from SW-13, NIH/3T3, Jurkat and PC-12 cells using PDK1 Antibody #3062. Western blot分析SW-13, NIH/3T3, Jurkat 和 PC-12 细胞，所用抗体为PDK1 Antibody #3062。 | |
Western blot analysis of extracts from PC3 cells, untreated or λ phosphatase-treated, using Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb #3438 (upper), PDK1 Antibody #3062 (middle) or Akt Antibody #9272 (lower). Western blot分析PC3细胞提取物，未处理组或λ 磷酸酶处理组，所用Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb兔单抗 #3438 (上), PDK1 Antibody #3062 (中)或Akt Antibody #9272 (下). | |
Western blot analysis of extracts from various cell lines using PTEN (D4.3) XP® Rabbit mAb #9188 (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower). Western blot分析不同细胞提取物，所用抗体为PTEN (D4.3) XP® Rabbit mAb兔单抗 #9188 (上) 和 Akt (pan) (C67E7) Rabbit mAb 兔单抗#4691 (下)。 | |
Western blot analysis of extracts from HeLa and NIH/3T3 cells using Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb #9549. Membranes were left untreated (-) or treated with (+) calf intestinal phosphatase (CIP) following western transfer to verify phospho-specificity of the antibody. Western blot分析HeLa 和 NIH/3T3 细胞提取物，所用抗体为-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb 兔单抗#9549。转膜后，膜分为未处理组（-）或小牛肠磷酸酶处理组（+）来证实抗体的磷酸化特异性。 | |
Western blot analysis of extracts from PC3 cells, untreated or λ phosphatase-treated, using Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb (upper), PDK1 Antibody #3062 (middle) or Akt Antibody #9272 (lower). | |
Immunohistochemical analysis using PTEN (D4.3) XP® Rabbit mAb on SignalSlide(TM) PTEN IHC Controls #8106 (paraffin-embedded LNCaP (left) and NIH/3T3 (right) cells). | |
Immunohistochemical analysis of paraffin-embedded human colon using PTEN (D4.3) XP® Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using PTEN (D4.3) XP® Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower). | |
Western blot analysis of extracts from HeLa and NIH/3T3 cells, using Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb. Membranes were either left untreated (-) or treated with (+) calf intestinal phosphatase (CIP) post Western transfer to verify phospho-specificity of the antibody. | |
Western blot analysis of extracts from SW-13, NIH/3T3, Jurkat and PC12 cells, using PDK1 Antibody. | |
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition. |
