| 货号 | 9779T |
| 描述 | The Pim Kinase Antibody Sampler Kit provides an economical means to detect all three Pim kinases along with Bad and Phospho-Bad (Ser112). The kit contains enough primary and secondary antibody to perform four western blot experiments. |
| 目标/特异性 | The Pim-1 (C93F2) Rabbit mAb, Pim-2 (D1D2) XP® Rabbit mAb, and Pim-3 (D17C9) Rabbit mAb detect endogenous levels of the respective proteins. The Pim antibodies do not crossreact with other Pim family members. Phospho-Bad (Ser112) (40A9) Rabbit mAb detects endogenous levels of Bad only when phosphorylated at Ser112. It does not detect Bad phosphorylated at other sites, nor does it detect related family members. Bad (D24A9) Rabbit mAb detects endogenous levels of total Bad protein. |
| 供应商 | CST |
| 背景 | Pim proteins (Pim-1, Pim-2 and Pim-3) are oncogene-encoded serine/threonine kinases (1). Pim-1, a serine/threonine kinase highly expressed in hematopoietic cells, plays a critical role in the transduction of mitogenic signals and is rapidly induced by a variety of growth factors and cytokines (1-4). Pim-1 cooperates with c-Myc in lymphoid cell transformation and protects cells from growth factor withdrawal and genotoxic stress-induced apoptosis (5,6). Pim-1 also enhances the transcriptional activity of c-Myb through direct phosphorylation within the c-Myb DNA binding domain as well as phosphorylation of the transcriptional coactivator p100 (7,8). Hypermutations of the Pim-1 gene are found in B-cell diffuse large cell lymphomas (9). Phosphorylation of Pim-1 at Tyr218 by Etk occurs following IL-6 stimulation and correlates with an increase in Pim-1 activity (10). Various Pim substrates have been identified; Bad is phosphorylated by both Pim-1 and Pim-2 at Ser112 and this phosphorylation reverses Bad-induced cell apoptosis (11,12). |
| 存放说明 | -20C |
| 参考文献 | Mikkers, H. et al. (2004) Mol Cell Biol 24, 6104-15. Selten, G. et al. (1986) Cell 46, 603-11. Meeker, T.C. et al. (1987) J Cell Biochem 35, 105-12. Dautry, F. et al. (1988) J Biol Chem 263, 17615-20. Möröy, T. et al. (1993) Proc Natl Acad Sci USA 90, 10734-8. Lilly, M. and Kraft, A. (1997) Cancer Res 57, 5348-55. Leverson, J.D. et al. (1998) Mol Cell 2, 417-25. Winn, L.M. et al. (2003) Cell Cycle 2, 258-62. Pasqualucci, L. et al. (2001) Nature 412, 341-6. Kim, O. et al. (2004) Oncogene 23, 1838-44. Aho, T.L. et al. (2004) FEBS Lett 571, 43-9. Yan, B. et al. (2003) J Biol Chem 278, 45358-67. Saris, C.J. et al. (1991) EMBO J 10, 655-64. |
Western blot analysis of extracts from COS cells, untreated or TPA-treated, using Phospho-Bad (Ser112) (40A9) Rabbit mAb #5284 (upper), or Bad Antibody #9292 (lower). | |
Western blot analysis of extracts from various cell lines using Pim-3 (D17C9) Rabbit mAb #4165. | |
Western blot analysis of extracts from various cell lines using Bad (D24A9) Rabbit mAb #9239. | |
Western blot analysis of extracts from various cell lines using Pim-1 (C93F2) Rabbit mAb #3247. | |
Western blot analysis of extracts from K-562, Raji, and KARPAS-620 cell lines using Pim-2 (D1D2) XP® Rabbit mAb #4730. | |
Western blot analysis of extracts from various cell lines using Pim-3 (D17C9) Rabbit mAb. | |
Western blot analysis of recombinant Pim-1, 2, and 3 using Pim-3 (D17C9) Rabbit mAb. | |
Western blot analysis of extracts from COS-7 cells, untransfected () or transfected with a mouse Pim-3 construct (+), using Pim-3 (D17C9) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using Bad (D24A9) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using Pim-1 (C93F2) Rabbit mAb. | |
Western blot analysis of recombinant Pim-1, -2, and -3 using Pim-1 (C93F2) Rabbit mAb. | |
Western blot analysis of recombinant Pim-1, Pim-2 and Pim-3 kinases using Pim-2 (D1D2) Rabbit mAb. | |
Western blot analysis of extracts from K-562, Raji and KARPAS-620 cell lines using Pim-2 (D1D2) Rabbit mAb. | |
Western blot analysis of extracts from COS cells, untreated or TPA-treated, using Phospho-Bad (Ser112) (40A9) Rabbit mAb (upper) or Bad Antibody #9292 (lower). | |
Flow cytometric analysis of COS cells, untreated (blue) or TPA/Calyculin A treated (green), using Phospho-Bad (Ser112) (40A9) Rabbit mAb. |
