mTOR Substrates Antibody Sampler Kit【科瑞奥博】

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mTOR Substrates Antibody Sampler Kit

货号： 9862T 基本售价： 4822.0 元规格： -

产品信息

概述
货号	9862T
描述	The mTOR Substrates Antibody Sampler Kit provides an economical means to evaluate the signaling of mTOR to downstream substrates including p70 S6 Kinase and 4E-BP1. The kit contains enough primary and secondary antibodies to perform four Western blot experiments per primary antibody.
目标/特异性	Each antibody in the mTOR Substrates Antibody Sampler Kit detects endogenous levels of its target protein. While activation state antibodies typically detect only target proteins phosphorylated at indicated residues, some cross-reaction can occur with related proteins phosphorylated at analogous sites.

性能
供应商	CST
背景	The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9).
存放说明	-20C
参考文献	Sabers, C.J. et al. (1995) J Biol Chem 270, 815-22. Brown, E.J. et al. (1994) Nature 369, 756-8. Sabatini, D.M. et al. (1994) Cell 78, 35-43. Gingras, A.C. et al. (2001) Genes Dev 15, 807-26. Dennis, P.B. et al. (2001) Science 294, 1102-5. Fang, Y. et al. (2001) Science 294, 1942-5. Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31. Peterson, R.T. et al. (2000) J Biol Chem 275, 7416-23. Huang, S. and Houghton, P.J. (2003) Curr Opin Pharmacol 3, 371-7. Hara, K. et al. (2002) Cell 110, 177-89. Kim, D.H. et al. (2002) Cell 110, 163-75. Beugnet, A. et al. (2003) J Biol Chem 278, 40717-22. Nojima, H. et al. (2003) J Biol Chem 278, 15461-4. Oshiro, N. et al. (2004) Genes Cells 9, 359-66. Kim, D.H. et al. (2003) Mol Cell 11, 895-904. Sarbassov, D.D. et al. (2004) Curr Biol 14, 1296-302. Sarbassov, D.D. et al. (2005) Science 307, 1098-101.

参考图片

Immunohistochemical analysis of paraffin-embedded mouse brain using mTOR (7C10) Rabbit mAb.

Western blot analysis of extracts from serum-starved NIH/3T3 cells, untreated or insulin-treated (150 nM, 5 minutes), alone or in combination with λ-phosphatase, using Phospho-mTOR (Ser2448) (D9C2) XP® Rabbit mAb #5536 (upper) or mTOR (7C10) Rabbit mAb #2983.

Western blot分析血清饥饿的NIH/3T3细胞，未处理组和胰岛素处理组 (150 nM, 5 分钟), 单独或与λ-磷酸酶联合组，所用抗体为Phospho-mTOR (Ser2448) (D9C2) XP® Rabbit mAb兔单抗 #5536 (上) 或 mTOR (7C10) Rabbit mAb兔单抗 #2983

Confocal immunofluorescent analysis of mouse embryonic fibroblast (MEF) cells using mTOR (7C10) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Western blot analysis of extracts from serum-starved NIH/3T3 cells, untreated or insulin-treated (150 nM, 5 minutes), alone or in combination with λ-phosphatase, using Phospho-mTOR (Ser2448) (D9C2) XP^® Rabbit mAb (upper) or mTOR (7C10) Rabbit mAb #2983.

Confocal immunofluorescent analysis of HeLa cells, rapamycin-treated (#9904, 10 μM for 2 hours, left), insulin-treated (150 nM for 6 minutes, middle) or insulin- and λ-phosphatase-treated (right), using Phospho-mTOR (Ser2448) (D9C2) XP^® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin. Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence^® mTOR siRNA II (+), using mTOR (7C10) Rabbit mAb #2983 and α-Tubulin (11H10) Rabbit mAb #2125. mTOR (7C10) Rabbit mAb confirms silencing of mTOR expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of mTOR siRNA.

Western blot analysis of extracts from 293T cells using 4E-BP1 Antibody #9452 (upper) and Phospho-4E-BP1 (Thr37/46) Antibody #2855 (lower). The cells were starved for 24 hours in serum-free medium and underwent a 1 hour amino acid deprivation. Amino acids were replenished for 1 hour. Cells were then either untreated (-) or treated with 100 nM insulin (+) for 30 minutes.western blot分析293T细胞提取物，所用抗体为4E-BP1 Antibody #9452 (u上) 和 Phospho-4E-BP1 (Thr37/46) Antibody #2855 (下). 细胞在无血清的培养液中饥饿24小时后，去除氨基酸1小时。在加入氨基酸1小时。未处理或采用100 nM insulin (+) 处理30分钟。

Western blot analysis of lysates from 293 cells grown in low serum, then treated with 20% serum for 30 minutes alone or after 1 hour preincubation with rapamycin (10 nM) #9904 or LY294002 (50 uM) #9901, using Phospho-p70 S6 Kinase (Ser371) Antibody #9208 (upper) or p70 S6 Kinase Antibody #9202 (lower).western blot分析生长在低浓度血清中的293细胞，然后用20%的血清单独处理30分钟，或与雷帕霉素(10 nM) #9904 or LY294002 (50 uM) #9901预孵育1小时，所用抗体为Phospho-p70 S6 Kinase (Ser371) Antibody #9208 (上) 或 p70 S6 Kinase Antibody #9202 (下)

Western blot analysis of extracts from serum starved or serum treated (20%) 293, NIH/3T3, and PC12 cells, using Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb #9234 (upper) or p70 S6 Kinase (49D7) Rabbit mAb #2708 (lower).

Western blot 分析血清饥饿和20%血清处理的293, NIH/3T3, 和 PC12 细胞提取物，所用抗体为Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb兔单抗 #9234 (上) 或 p70 S6 Kinase (49D7) Rabbit mAb 兔单抗#2708 (下)。

Western blot analysis of extracts from various cell types using mTOR (7C10) Rabbit mAb #2983.

western blot分析不同细胞提取物，所用抗体为mTOR (7C10) Rabbit mAb兔单抗 #2983

Confocal immunofluorescent analysis of HeLa cells treated with LY294002 (left) or 20% serum (right) and labeled with Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb in the presence of control peptide (left) or Phospho-4E-BP1 (Thr37/46) Blocking Peptide #1052 (right).

Flow cytometric analysis of Jurkat cells, untreated (green) or LY294002, Wortmannin and U0126-treated (blue), using Phospho-4E-BP1 (Thr36/46) (236B4) Rabbit mAb compared to a nonspecific negative control antibody (red).