| 货号 | 9962T |
| 描述 | The Epitope Tag Antibody Sampler Kit provides an economical means to analyze the expression of a variety of epitope tagged proteins. The kit contains enough primary and secondary antibodies to perform four Western blots per primary antibody. |
| 目标/特异性 | All antibodies in the Epitope Tag Antibody Sampler Kit detect overexpressed fusion proteins containing the corresponding epitope tags. DYKDDDDK Tag Antibody recognizes the DYKDDDDK peptide (the same epitope recognized by Sigma’s Anti-FLAG® antibodies), and its binding specificity is NOT dependent on the presence of divalent metal cations. |
| 供应商 | CST |
| 背景 | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged proteins biochemical properties. |
| 存放说明 | -20C |
| 参考文献 | Kroll, D.J. et al. (1993) DNA Cell Biol. 12, 441-453. di Guan, C. et al. (1988) Gene 67, 21-30. Guan, K.L. and Dixon, J.E. (1991) Anal. Biochem. 192, 262-267. Davis, A.H. et al. (1993) Biotechnology 11, 933-936. Yu, J. et al. (1998) Mol. Cell. Biol. 18, 1379-1387. Field, J. et al. (1988) Mol. Cell. Biol. 8, 2159-2165. Munro, S. and Pelham, H.R. (1984) EMBO J. 3, 3087-3093. Brizzard, B. L. et al. (1994) Biotechniques 16, 730-735. |
293T cells were either untransfected (left panel) or transfected with an His-tagged human Stat3 construct (right panel), then treated with Human Interleukin-6 (hIL-6) #8904 (100 ng/ml, 30 min). Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 cells and 5 µl of His-Tag (D3I1O) XP® Rabbit mAb, 5 µl of Stat3α (D1A5) XP® Rabbit mAb #8768, or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human c-Fos Promoter Primers #4663, human IRF-1 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. | |
Flow cytometric analysis of 293T cells, untransfected (blue) or transfected with a His-myc-Akt plasmid (green), using His-Tag (D3I1O) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody. | |
Immunoprecipitation of His-Tag protein from transfected 293T cells using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or His-Tag (D3I1O) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using His-Tag (D3I1O) XP® Rabbit mAb. | |
Western blot analysis of extracts from 293T cells, untransfected or transfected with 6xHis-Tag fusion protein 1 or 6xHis-Tag fusion protein 2, using His-Tag (D3I1O) XP® Rabbit mAb. | |
Confocal immunofluorescent analysis of 293T cells transfected with a His-Tagged protein using His-Tag (D3I1O) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from cells expressing C-terminal His-tagged protein (lane 1) or control extract (lane 2), using His-Tag Antibody #2365. | |
Western blot analysis of extracts from HeLa cells, untransfected or transfected with either HA-FoxO4 or HA-Akt3, using HA-Tag (C29F4) Rabbit mAb #3724. | |
Western blot analysis of extracts from 293T cells, untransfected or transfected with either DYKDDDDK-tagged FoxG1, NPAS4, or Tyrosine Hydroxylase (TH), using DYKDDDDK Tag (9A3) Mouse mAb #8146. | |
293T cells were either untransfected (left panel) or transfected with an HA-tagged human CBP construct (right panel), then treated with Forskolin #3828 (30 µM) . Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 cells and 10 µl of HA-Tag (C29F4) Rabbit mAb, 10 µl of CBP (D9B6) Rabbit mAb, or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers, SimpleChIP® Human NR4A3 Promoter Primers #4829, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. | |
Flow cytometric analysis of Cos cells, untreated (blue) or transfected with a DYKDDDDK-tagged IRF3 contruct (green) using DYKDDDDK Tag (9A3) Mouse mAb. | |
Immunohistochemical analysis of paraffin-embedded 293T cell pellets, control (left) or ROS-DYKDDDDK Tag transfected (right), using DYKDDDDK Tag (9A3) Mouse mAb. | |
Confocal immunofluorescent analysis of HeLa cells transfected with a DYKDDDDK-tagged IRF3 construct using DYKDDDDK Tag (9A3) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from 293T cells, untransfected or transfected with either DYKDDDDK-tagged FoxG1, NPAS4, or Tyrosine Hydroxylase (TH), using DYKDDDDK Tag (9A3) Mouse mAb. | |
Confocal immunofluorescent analysis of COS cells, transfected with an HA-tagged protein (left) or mock-transfected (right), using HA-Tag (C29F4) Rabbit mAb (green). Actin filaments have been labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye). | |
Western blot analysis of extracts from HeLa cells, untransfected or transfected with either HA-FoxO4 or HA-Akt3, using HA-Tag (C29F4) Rabbit mAb. |
