| 货号 | 32345T |
| 目标/特异性 | Atg5 (D5F5U) Rabbit mAb recognizes endogenous levels of total Atg5 protein. This antibody is capable of detecting Atg5 conjugated to Atg12. Atg12 (D88H11) Rabbit mAb detects endogenous levels of total free and Atg5 bound Atg12 protein. Atg16L1 (D6D5) Rabbit mAb recognizes endogenous levels of total Atg16L1 protein. A background band is detected at 40 kDa in some cell lines. Atg7 (D12B11) Rabbit mAb recognizes endogenous levels of total Atg7 protein. |
| 供应商 | CST |
| 背景 | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8). Atg16L1 binds Atg5 of the Atg12-Atg5 conjugate forming an 800 kDa multimeric complex (9). The Atg12-Atg-5-Atg16L1 complex localizes to pre-autophagosomal membranes where it determines the site of LC3 lipidation and catalyzes the reaction required for the formation of mature autophagosomes (9,10). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 参考文献 | 1 . Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21. 2 . Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18. 3 . Levine, B. and Yuan, J. (2005) J Clin Invest 115, 2679-88. 4 . Mizushima, N. et al. (2003) J Cell Sci 116, 1679-88. 5 . Mizushima, N. et al. (1998) J Biol Chem 273, 33889-92. 6 . Mizushima, N. et al. (1998) Nature 395, 395-8. 7 . Fujita, N. et al. (2008) Mol Biol Cell 19, 2092-100. 8 . Suzuki, K. et al. (2001) EMBO J 20, 5971-81. 9 . Tanida, I. et al. (1999) Mol Biol Cell 10, 1367-79. 10 . Shintani, T. et al. (1999) EMBO J 18, 5234-41. |
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition. | |
Western blot analysis of extracts from various cell lines using Atg12 (D88H11) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using Atg7 (D12B11) Rabbit mAb. | |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® Atg7 siRNA I #6604 (+), using Atg7 (D12B11) Rabbit mAb (upper) or α-Tubulin (11Η10) Rabbit mAb #2125 (lower). The Atg7 (D12B11) Rabbit mAb confirms silencing of Atg7 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. | |
Western blot analysis of extracts from various cell lines using Atg16L1 (D6D5) Rabbit mAb. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with either a myc-tagged human Atg16L1β construct (hAtg16L1β-Myc; +) or a mouse Atg16L1α construct (mAtg16L1α; +), using Atg16L1 (D6D5) Rabbit mAb. The myc-tagged human Atg16L1β construct was kindly provided by Dr. Qing Zhong, University of California Berkeley. | |
Confocal immunofluorescent analysis of EBSS-starved PANC-1 cells using Atg16L1 (D6D5) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines using Atg5 (D5F5U) Rabbit mAb. | |
Immunoprecipitation of Atg5 from PANC-1 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Atg5 (D5F5U) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Atg5 (D5F5U) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody to avoid cross-reactivity with rabbit IgG. | |
Western blot analysis of extracts from wild-type MEFs (wt) or MEFs from Atg5 knockouts (Atg5-/-) using Atg5 (D5F5U) Rabbit mAb (upper), or β-Actin (D6A8) Rabbit mAb #8457 (lower). Atg5-/- MEFs were kindly provided by Dr. Ramnik Xavier, Massachusetts General Hospital, Harvard Medical School, Boston, MA. | |
Western blot analysis of extracts from various cell lines using Atg5 (D5F5U) Rabbit mAb #12994. | |
Western blot analysis of extracts from various cell lines using Atg16L1 (D6D5) Rabbit mAb #8089. | |
Western blot analysis of extracts from various cell lines using Atg12 (D88H11) Rabbit mAb #4180. | |
Western blot analysis of extracts from various cell lines using Atg7 (D12B11) Rabbit mAb #8558. |
