| 货号 | 12704T |
| 目标/特异性 | Each antibody recognizes endogenous levels of their target protein. Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb recognizes endogenous levels of acetyl-CoA carboxylase protein only when phosphorylated at Ser79. |
| 供应商 | CST |
| 背景 | Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA (1). It is the key enzyme in the biosynthesis and oxidation of fatty acids (1). In rodents, the 265 kDa ACC1 (ACCα) form is primarily expressed in lipogenic tissues, while 280 kDa ACC2 (ACCβ) is the main isoform in oxidative tissues (1,2). However, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues (1,2). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). ACC is a potential target of anti-obesity drugs (4,5). |
| 存放说明 | -20C |
| 参考文献 | Castle, J.C. et al. (2009) PLoS One 4, e4369. Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86. Ha, J. et al. (1994) J Biol Chem 269, 22162-8. Abu-Elheiga, L. et al. (2001) Science 291, 2613-6. Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50. |
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition. | |
Flow cytometric analysis of 293 cells, using Acetyl-CoA Carboxylase (C83B10) Rb mAb (blue) compared to a nonspecific negative control antibody (red). | |
Western blot analysis of cell extracts from various cell lines, using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb. | |
Confocal immunofluorescent analysis of NIH/3T3 cells labeled with Acetyl-CoA Carboxylase (C83B10) Rabbit mAb (red). Blue pseudocolor=Draq5™ (fluorescent DNA dye). | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma, using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb in the presence of control peptide (left) or Acetyl-CoA Carboxylase (C83B10) Blocking Peptide #1062 (right). | |
Western blot analysis of extracts from various cell types using Acetyl-CoA Carboxylase 1 Antibody. | |
Western blot analysis of extracts from HeLa cells, mock transfected or transfected with either SignalSilence® ACC1 siRNA or SignalSilence®ACC2 siRNA, using Acetyl-CoA Carboxylase 1 Antibody and β-Actin (13E5) Rabbit mAb #4970. | |
Western blot analysis of extracts from human adipocytes using Acetyl-CoA Carboxylase 2 (D5B9) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded NCI-H2228 cell pellets, untreated (left) or phenformin-treated (right), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, untreated (left) or λ phosphatase-treated (right), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb. | |
Western blot analysis of extracts from SH-SY5Y cells, untreated or treated with Oligomycin #9996 (0.5 μM, 30 min), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb (upper) or Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676 (lower). The phospho-specificity of the antibody was verified by λ phosphatase treatment. |
