Notch Isoform Antibody Sampler Kit【科瑞奥博】

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Notch Isoform Antibody Sampler Kit

货号： 3640T 基本售价： 4018.0 元规格： -

产品信息

概述
货号	3640T
描述	The Notch Isoform Antibody Sampler Kit provides an economical means to investigate Notch Signaling. The kit contains primary and secondary antibodies to perform four western mini-blots with each antibody.
目标/特异性	Cleaved Notch1 (V1744) (D3B8) Rabbit mAb detects endogenous levels of the Notch1 intra- cellular domain (NICD) only when released by cleavage between Gly1753 and Val1754 (equivalent to Gly1743/Val1744 of murine notch1). The antibody does not recognize full- length Notch1 or Notch1 cleaved at other positions. The size of the NICD varies among cell lines due to mutations in the Notch1 C-terminus (6). Notch1 (D1E11) XP® Rabbit mAb detects endogenous levels of total Notch1 protein. It recognizes both the full-length (~300 kDa) and the trans- membrane/intracellular region NTM (~120 kDa). Notch2 (D76A6) XP® Rabbit mAb detects endogenous levels of total Notch2 protein. It recognizes both the full-length (~ 300 kDa) and the trans- membrane/intracellular region NTM (~110 kDa). Notch3 (D11B8) Rabbit mAb detects endogenous levels of total Notch3 protein. The antibody recognizes both full-length (FL) Notch3 at 270 kDa and a truncated protein (NTM) containing a short extracellullar region, the transmembrane domain and the intracellular region at 90 kDa.

性能
供应商	CST
背景	Notch1 is a transmembrane protein functioning in development and the determination of cell fate (1). During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription.
存放说明	-20C
参考文献	Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6. Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6. Schroeter, E.H. et al. (1998) Nature 393, 382-6. Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35. Weng, A.P. et al. (2004) Science 306, 269-71. Thompson, B.J. et al. (2007) J Exp Med 204, 1825-35. McDaniell, R. et al. (2006) Am J Hum Genet 79, 169-73. Baron, M. (2003) Semin Cell Dev Biol 14, 113-9. Kalimo, H. et al. (2002) Brain Pathol 12, 371-84. Karlström, H. et al. (2002) Proc Natl Acad Sci U S A 99, 17119-24. Monet, M. et al. (2007) Hum Mol Genet 16, 982-92. Park, J.T. et al. (2006) Cancer Res 66, 6312-8. Gramantieri, L. et al. (2007) Liver Int 27, 997-1007. Yamaguchi, N. et al. (2008) Cancer Res 68, 1881-8.

参考图片

Western blot analysis of extracts from 293 and SK-MEL-5 cells using Notch2 (D76A6) XP® Rabbit mAb #5732. The full-length (FL) Notch2 protein and the transmembrane/intracellular region (NTM) are indicated.

CUTLL1 cells were cultured in media with γ-secretase inhibitor (1 μM) for 3 days and then either harvested immediately (left panel) or washed and cultured in fresh media for 3 hr (right panel). Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10⁶ cells and 5 µl of Notch1 (D1E11) XP^® Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP^® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human HES1 promoter primers, SimpleChIP^® Human HES4 Promoter Primers #7273, and SimpleChIP^® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Western blot analysis of extracts from various cell lines using Notch3 (D11B8) XP® Rabbit mAb #5276.使用Notch3 (D11B8) XP®兔单抗#5276对多种细胞提取物进行western blot分析。

CUTLL1 cells were cultured in media with γ-secretase inhibitor (1μM) for 3 days and then either harvested immediately (left panel) or washed and cultured in fresh media for 3h (right panel). Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10⁶ cells and 2.5 µl of Cleaved Notch1 (Val1744) (D3B8) Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP^® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human HES1 promoter primers, SimpleChIP^® Human HES4 Promoter Primers #7273, and SimpleChIP^® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Flow cytometric analysis of SW620 (red) and 293 (blue) cells using Notch2 (D76A6) XP^® Rabbit mAb.

Western blot analysis of extracts from 293 and SK-MEL-5 cells using Notch2 (D76A6) XP^® Rabbit mAb. The full-length (FL) Notch2 protein and the transmembrane/intracellular region (NTM) are indicated.

Western blot analysis of extracts from HBP-ALL, and Molt4 using Notch1 (D6F11) XP® Rabbit mAb #4380. The full-length (FL) and transmembrane/intracellular region (NTM) are indicated.使用Notch1 (D6F11) XP®兔单抗#4380对HBP-ALL和Molt4细胞提取物进行western blot分析。全长（FL）和跨膜/细胞内部位（NTM）如图所示。

Immunohistochemical analysis of paraffin-embedded human colon using Notch1 (D1E11) XP^® Rabbit mAb.

Confocal immunofluorescent analysis of SNB19 cells using Notch2 (D76A6) XP^® Rabbit mAb (green). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Western blot analysis of extracts from various cell lines using Notch3 (D11B8) Rabbit mAb.

Western blot analysis of extracts from various cell lines using Cleaved Notch1 (Val1744) (D3B8) Rabbit mAb (upper) or Notch1 (D1E11) XP^® Rabbit mAb #3608 (lower).

Western blot analysis of extracts from various cell lines using Cleaved Notch1 (Val1744) (D3B8) XP® Rabbit mAb #4147 (upper) or Notch1 (D1E11) Rabbit mAb #3608 (lower).使用剪切后Notch1 (Val1744) (D3B8) XP®兔单抗#4147（上）或Notch1 (D1E11)兔单抗#3608（下）对多种细胞提取物进行western blot分析。

Western blot analysis of total cell extract from various cell types using Notch1 (D1E11) XP^® Rabbit mAb. The full-length (FL) Notch protein and the cleaved transmembrane/intracellular region (NTM) are indicated.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Notch1 (D1E11) XP^® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human inflammatory granulation tissue using Notch1 (D1E11) XP^® Rabbit mAb.