| 货号 | 4769T |
| 描述 | The IRAK Isoform Antibody Sampler Kit provides an economical means to examine total protein levels of the four Interleukin-1 Receptor Associated Kinase family members: IRAK1, IRAK2, IRAK3/IRAK-M, and IRAK4. |
| 目标/特异性 | Each antibody in the IRAK Isoform Antibody Sampler Kit detects endogenous levels of its indicated target. Cross-reactivity has not been detected with other family members at endogenous levels. |
| 供应商 | CST |
| 背景 | Interleukin-1 (IL-1) receptor-associated kinase (IRAK) is a serine/threonine-specific kinase that can be coprecipitated in an IL-1-inducible manner with the IL-1 receptor (1). The mammalian family of IRAK molecules contains four members (IRAK1, IRAK2, IRAK3/IRAK-M and IRAK4). The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1RI, AcP, MyD88 and IRAKs (2). IRAK undergoes autophosphorylation shortly after IL-1 stimulation. The subsequent events involve IRAK dissociation from the IL-1RI complex, its ubiquitination and its association with two membrane-bound proteins: TAB2 and TRAF6. The resulting IRAK-TRAF6-TAB2 complex is then released into the cytoplasm and activates protein kinase cascades, which include TAK1, IKKs and the stress-activated kinases (3). |
| 存放说明 | -20C |
| 参考文献 | Dinarello, C.A. (1996) Blood 87, 2095-147. Takaesu, G. et al. (2001) Mol Cell Biol 21, 2475-84. Janssens, S. and Beyaert, R. (2003) Mol Cell 11, 293-302. Gottipati, S. et al. (2008) Cell Signal 20, 269-76. Kollewe, C. et al. (2004) J Biol Chem 279, 5227-36. Wesche, H. et al. (1999) J Biol Chem 274, 19403-10. Muzio, M. et al. (1997) Science 278, 1612-5. Guo, F. et al. (1999) Inflammation 23, 535-43. Kobayashi, K. et al. (2002) Cell 110, 191-202. |
Flow cytometric analysis of untreated Raw264.7 cells, +GFP shRNA (blue) or IRAK1 shRNA (green), using IRAK1 (D51G7) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 fragment (Alexa Fluor® 647 conjugate) #4414 was used as a secondary Ab. | |
Western blot analysis of extracts from THP-1, mouse embryonic fibroblast (MEF), RAW264.7 and NIH/3T3 cells using IRAK1 (D51G7) XP® Rabbit mAb #4504.Western免疫印迹。用 IRAK1 (D51G7) XP® Rabbit mAb #4504研究THP1 和小鼠纤维原细胞(MEF), RAW264.7和NIH/3T3细胞的细胞提取液。 | |
Western blot analysis of extracts from THP-1, mouse embryonic fibroblast (MEF), RAW264.7 and NIH/3T3 cells using IRAK1 (D51G7) XP® Rabbit mAb. | |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® IRAK1 siRNA I (+) or SignalSilence® IRAK1 siRNA II #6228 (+), using IRAK1 (D51G7) XP® Rabbit mAb #4504 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The IRAK1 (D51G7) XP® Rabbit mAb confirms silencing of IRAK1 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. | |
Western blot analysis of extracts from RD, 293 and A20 cells using IRAK1 (D51G7) XP® Rabbit mAb. | |
Western blot analysis of extracts from COS-7 cells, either mock transfected or transfected with human IRAK1, using IRAK1 (D51G7) XP® Rabbit mAb. | |
Western blot analysis of extracts from Jurkat, Raji and K562 cell lines, using IRAK2 Antibody #4367.Western免疫印迹。用IRAK2 Antibody #4367研究Jurkat, Raji 和 K562 细胞系的细胞提取液。 | |
Western blot analysis of extracts from HL-60, Raji and K562 cell lines, using IRAK-M Antibody #4369.Western免疫印迹。用IRAK-M Antibody #4369研究HL-60, Raji 和 K562 细胞系的细胞提取液。 | |
Western blot analysis of extracts from THP-1 (human), RAW 264.7 (mouse), and H-4-II-E (rat) cell lines, using IRAK4 Antibody #4363.Western免疫印迹。用IRAK4 Antibody #4363研究THP1 （人源）， RAW264.7（小鼠源）和H-4-II-E（大鼠源）细胞系的细胞提取液。 | |
Immunofluorescent staining of HeLa cells, using IRAK2 Antibody. | |
Western blot analysis of extracts from Jurkat, Raji and K562 cell lines, using IRAK2 Antibody #4367. | |
Western blot analysis of extracts from HL-60, Raji and K562 cell lines, using IRAK-M Antibody. | |
Immunofluorescent analysis of THP-1 cells differentiated overnight with TPA, using IRAK-M Antibody. | |
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition. | |
Western blot analysis of extracts from THP-1 (human), RAW 264.7 (mouse), and H-4-II-E (rat) cell lines, using IRAK4 Antibody. |
