| 货号 | 5887T |
| 描述 | The γ Secretase Antibody Sampler Kit provides an economical means of evaluating components of the gamma secretase complex. The kit contains enough primary and secondary antibodies to perform four western miniblot experiments. γ Secretase Antibody Sampler Kit 小包装抗体试剂盒提供了一个经济的手段来研究γ-分泌酶复合体。该试剂盒包含了足够的一抗和二抗以满足每款抗体用于4张膜的免疫印迹实验。 |
| 目标/特异性 | Presenilin 1 (D39D1) Rabbit mAb recognizes endogenous levels of total Presenilin 1 protein. The antibody may detect a nonspecific band at 60 kDa. Presenilin 2 Antibody detects endogenous levels of the carboxy-terminal fragment of Presenilin 2 (23 kDa) and the full length protein (54 kDa) to a lesser extent. It does not cross-react with endogenous levels of presenilin 1. Nicastrin (D38F9) Rabbit mAb recognizes endogenous levels of total Nicastrin protein. PEN2 Antibody detects endogenous levels of total PEN2 protein. |
| 供应商 | CST |
| 背景 | The γ secretase protease complex interacts with and cleaves intramembrane substrates as an essential function for regulation of intracellular signaling and cell-cell interactions. This multiprotein complex is comprised of four integral membrane proteins, Presenilin, Nicastrin, Aph-1, and PEN2, all of which are essential for complete proteolytic activity (1). Presenilin 1 and presenilin 2 are transmembrane proteins belonging to the presenilin family. Mutation of presenilin genes has been linked to early onset of Alzheimer disease, probably due to presenilin’s associated γ-secretase activity for amyloid-β protein processing (2,3). Endogenous presenilin mainly exists in a heterodimeric complex formed from the endoproteolytically processed amino-terminal (34 kDa) and carboxy-terminal (~20, 22, 23 kDa) fragments (CTF) (3,4). Nicastrin is a transmembrane glycoprotein serving as an essential component of the γ-secretase complex (5,6). Nicastrin protein is physically associated with presenilin and plays an important role in stabilization and correct localization of presenilin to the membrane-bound γ-secretase complex (7). Nicastrin also serves as a docking site for γ-secretase substrates such as APP and Notch, directly binding to them and presenting them properly to γ-secretase to ensure the correct cleavage process (6,8). Presenilin Enhancer 2 (PEN2) is a small integral membrane glycoprotein that contains two recognized transmembrane domains. Both the N- and C-terminal domains are oriented into the lumen of the endoplasmic reticulum (9). PEN2 is an important part of the γ-secretase complex as its knock down results in reduced amounts of the complex, resulting in a loss of γ-secretase activity (10). |
| 存放说明 | -20C |
| 参考文献 | 1 . Yu, G. et al. (2000) Nature 407, 48-54. 2 . Sala Frigerio, C. et al. (2005) J Neurol 252, 1033-6. 3 . Hansson, C.A. et al. (2004) J Biol Chem 279, 51654-60. 4 . Haass, C. and De Strooper, B. (1999) Science 286, 916-9. 5 . Kimberly, W.T. et al. (2000) J. Biol. Chem. 275, 3173-3178. 6 . Esler, W. P. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 2720-2725. 7 . Kim, T.W. et al. (1997) J. Biol. Chem. 272, 11006-11010. 8 . Kopan, R. and Goate, A. (2002) Neuron 33, 321-324. 9 . Chen, F. et al. (2001) Nat. Cell Biol. 3, 751-754. 10 . Steiner, H. et al. (2002) J Biol Chem 277, 39062-5. |
Western blot analysis of extracts from various cell lines using PEN2 (D6G8) Rabbit mAb. | |
Western blot analysis of extracts from human (293 and HeLa), mouse (NIH/3T3 and L929) and rat (C6 and PC12) cell lines using Presenilin 2 Antibody #2192.对人293和HeLa细胞，小鼠NIH/3T3和L929细胞，大鼠C6和PC12细胞系，使用Presenilin 2抗体#2192进行Western blot分析。 | |
Western blot analysis of extracts from HeLa, wild-type MEF, and Presenilin 1(-/-) MEF cells using Presenilin 1 (D39D1) Rabbit mAb #5643 (upper) and α/β-Tubulin Antibody #2148 (lower). (Wild-type MEF and Presenilin 1(-/-) MEF cells were kindly provided by Dr. Bart De Strooper, K.U.Leuven, Belgium).对HeLa、野生型MEF和Presenilin 1(-/-) MEF细胞抽提液使用Presenilin 1 (D39D1) Rabbit mAb #5643（上图）及α/β-Tubulin Antibody #2148（下图）进行Western blot分析。（野生型MEF和Presenilin 1(-/-) MEF细胞由Dr.Bart De Strooper, K.U.Leuven, Belgium提供） | |
Western blot analysis of extracts from various cell lines using PEN2 Antibody #5451.对多个细胞系使用PEN2抗体#5451进行Western blot分析。 | |
Western blot analysis of extracts from Presenilin 1 (-/-) and Presenilin 2 (-/-) MEF cells using Presenilin 2 (D30G3) Rabbit mAb (upper) and Presenilin 1 (D39D1) Rabbit mAb #5643 (lower). (Presenilin 1 (-/-) and Presenilin 2 (-/-) MEF cells were kindly provided by Dr. Bart De Strooper, K.U.Leuven, Belgium). | |
Western blot analysis of extracts from various cell lines using Nicastrin (D38F9) Rabbit mAb #5665.对多个细胞系使用Nicastrin (D38F9)兔单克隆抗体#5665进行Western blot分析。 | |
Western blot analysis of extracts from HeLa, wild-type MEF, and Presenilin 1(-/-) MEF cells using Presenilin 1 (D39D1) Rabbit mAb (upper) and α/β-Tubulin Antibody #2148 (lower). (Wild-type MEF and Presenilin 1(-/-) MEF cells were kindly provided by Dr. Bart De Strooper, K.U.Leuven, Belgium). | |
Western blot analysis of extracts from various cell lines using Nicastrin (D38F9) Rabbit mAb. | |
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition. |
