| 货号 | 8686T |
| 描述 | The Lysine Acetyltransferase Antibody Sampler Kit provides an economical means to examine several lysine acetyltrasferases, including: Acetyl-CBP, CBP, GCN5L2, and PCAF. The kit contains enough primary antibody to perform four western blots per primary. |
| 目标/特异性 | Each antibody in the Lysine Acetyltransferase Antibody Sampler Kit recognizes endogenous levels of respective target protein. The antibodies do not cross-react with other family members. |
| 供应商 | CST |
| 背景 | CREB-binding protein (CBP) and p300 are highly conserved and functionally related transcriptional co-activators that associate with transcriptional regulators and signaling molecules, integrating multiple signal transduction pathways with the transcriptional machinery (1,2). CBP/p300 also contain histone acetyltransferase (HAT) activity, allowing them to acetylate histones and other proteins (2). The role of acetylation of CBP/p300 is of particular interest (2,3). Acetylation of p300 at Lys1499 has been demonstrated to enhance its HAT activity and affect a wide variety of signaling events (4). p300/CBP-associated factor (PCAF), also known as lysine acetyl-transferase 2B (KAT2B) (5), and General Control of Amino Acid Synthesis Yeast Homolog Like 2 (GCN5L2) (6) are transcriptional adaptor proteins in addition to HATs. PCAF functions as the catalytic subunit of the PCAF transcriptional co-activator complex (5). GCN5L2 functions as the catalytic subunit of the STAGA and TFTC transcription coactivator complexes (6). PCAF and GCN5L2 acetylate histone H3 at Lys14 and histone H4 at Lys8, both of which contribute to gene activation by modulating chromatin structure and recruiting additional co-activator proteins that contain acetyl-lysine binding bromo-domains (7,8). PCAF also acetylates non-histone proteins including transcriptional activators (p53, E2F1, MyoD) and general transcription factors (TFIIEβ and TFIIF) (9-12). GCN5L2 also acetylates non-histone proteins such as transcription activators (TAT, c-Myb) (13,14), transcription co-activators (PGC1-α) (15), and nuclear receptors (Steroidogenic Factor 1) (16). Acetylation of these proteins regulates their nuclear localization, protein stability, DNA binding, and co-activator association (13-16).Application References |
| 存放说明 | -20C |
| 参考文献 | 1 . Thompson, P.R. et al. (2004) Nat. Struct. Mol. Biol. 11, 308-315. 2 . Goodman, R.H. and Smolik, S. (2000) Genes Dev 14, 1553-77. 3 . Chan, H.M. and La Thangue, N.B. (2001) J. Cell Sci. 114, 2363-2373. 4 . Liu, L. et al. (1999) Mol Cell Biol 19, 1202-9. 5 . Nagy, Z. and Tora, L. (2007) Oncogene 26, 5341-57. 6 . Yuan, L.W. and Giordano, A. (2002) Oncogene 21, 2253-2260. 7 . Candau, R. et al. (1996) Mol Cell Biol 16, 593-602. 8 . Grant, P.A. et al. (1999) J Biol Chem 274, 5895-900. 9 . Bannister, A.J. and Miska, E.A. (2000) Cell Mol Life Sci 57, 1184-92. 10 . Lerin, C. et al. (2006) Cell Metab 3, 429-38. 11 . Sartorelli, V. et al. (1999) Mol Cell 4, 725-34. 12 . Imhof, A. et al. (1997) Curr Biol 7, 689-92. 13 . Schiltz, R.L. et al. (1999) J Biol Chem 274, 1189-92. 14 . Kiernan, R.E. et al. (1999) EMBO J 18, 6106-18. 15 . Tomita, A. et al. (2000) Oncogene 19, 444-51. 16 . Jacob, A.L. et al. (2001) J Biol Chem 276, 37659-64. |
Western blot analysis of extracts from various cell lines using GCN5L2 (C26A10) Rabbit mAb #3305.Western blot方法检测不同细胞系的提取物，所用抗体为 GCN5L2 (C26A10) Rabbit mAb #3305。 | |
Western blot analysis of extracts from various cell lines using PCAF (C14G9) Rabbit mAb #3378.Western blot方法检测不同细胞系的提取物，所用抗体为PCAF (C14G9) Rabbit mAb #3378。 | |
Western blot analysis of extracts from various cell lines using Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody #4771.Western blot方法检测不同细胞系的提取物，所用抗体为Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody #4771。 | |
Western blot analysis of extracts from various cell lines using CBP (D6C5) Rabbit mAb #7389.Western blot方法检测不同细胞系的提取物，所用抗体为CBP (D6C5) Rabbit mAb #7389。 | |
Western blot analysis of extracts from various cell lines using CBP (D6C5) Rabbit mAb. | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 293 cells, treated with Forskolin #3828 (30 μM, 1h) and either 20 μl of CBP (D6C5) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers, SimpleChIP® Human NR4A3 Promoter Primers #4829, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. | |
Confocal immunofluorescent analysis of HeLa cells using CBP (D6C5) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 293 cells treated with Forskolin #3828 (30uM) and either 20 μl of Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody or 2 μl of Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers, SimpleChIP® Human NR4A3 Promoter Primers #4829, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. | |
Western blot analysis of extracts from various cell lines using PCAF (C14G9) Rabbit mAb. | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 293 cells treated with Forskolin #3828 (30 µM) and either 20 μl of PCAF (C14G9) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers, SimpleChIP® Human NR4A3 Promoter Primers #4829, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. | |
Western blot analysis of extracts from various cell lines using GCN5L2 (C26A10) Rabbit mAb. | |
Confocal immunofluorescent analysis of HeLa cells using GCN5L2 (C26A10) Rabbit mAb (green). Actin filaments have been labeled with DY554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). | |
Western blot analysis of extracts from A431, NIH/3T3, COS and PC12 cells, using Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody. | |
Western blot analysis of hypo- or hyper-acetylated recombinant p300 HAT domains, either wild-type or K1499R mutant, using Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody (upper). Also shown in the corresponding coomassie stained SDS-PAGE gel (lower). (Details are described in Thompson, P.A. et al. (2004) Nat. Struct. Mol. Biol. 11, 308-315.) | |
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition. |
