| 货号 | 9326T |
| 目标/特异性 | RSK1/RSK2/RSK3 (32D7) Rabbit mAb detects endogenous levels of total RSK1-3 proteins. Each activation state monoclonal antibody recognizes its specific target; some cross-reactivity with related phosphorylated RSK proteins may occur. |
| 供应商 | CST |
| 背景 | The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, by autophosphorylation, and by phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3). |
| 存放说明 | -20C |
| 参考文献 | Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9. Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505. |
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Phospho-p90RSK (Ser380) (D3H11) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, control (left) or λ phosphatase-treated (right), using Phospho-p90RSK (Ser380) (D3H11) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded HeLa cell pellets, untreated (left) or treated with TPA #4174 (right), using Phospho-p90RSK (Ser380) (D3H11) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines, starved overnight and either untreated (-) or treated (+) with TPA #4174 (200 nM, 15 min), Human Epidermal Growth Factor (hEGF) #8916 (100 ng/mL, 15 min), or Human Platelet-Derived Growth Factor BB (hPDGF-BB) #8912 (100 ng/mL, 15 min) as indicated, using Phospho-p90RSK (Ser380) (D3H11) Rabbit mAb. | |
Immunoprecipitation of phospho-p90RSK (Thr359) from extracts of HeLa cells, serum starved overnight and either untreated (-) or treated with TPA #4174 (200 nM, 15 min; +), using Normal Rabbit IgG #2729 (lanes 5 and 6) or Phospho-p90RSK (Thr359) (D1E9) Rabbit mAb (lanes 3 and 4). Lanes 1 and 2 are 10% input. Western blot analysis was performed using RSK1/RSK2/RSK3 Antibody #9347. | |
Western blot analysis of extracts from HeLa, COS-7, and A-431 cells, starved overnight and either untreated (-) or treated (+) with TPA #4174 (200 nM, 15 min) or Human Epidermal Growth Factor (hEGF) #8916 (100 ng/mL, 15 min), using Phospho-p90RSK (Thr359) (D1E9) Rabbit mAb. | |
Confocal immunofluorescent analysis of NIH/3T3 cells, serum-starved (left), treated with TPA #4174 (200 nM, 15 min; center), or treated with TPA followed by λ phosphatase (right), using Phospho-p90RSK (Ser380) (D3H11) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Immunohistochemical analysis of paraffin-embedded colon carcinoma using Phospho-p90RSK (Thr359) (D1E9) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma, control (left) or λ phosphatase-treated (right), using Phospho-p90RSK (Thr359) (D1E9) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded A-431 cell pellets, untreated (left) or treated with hEGF #8916 (right), using Phospho-p90RSK (Thr359) (D1E9) Rabbit mAb. | |
Confocal immunofluorescent analysis of A-431 cells, serum-starved (left), treated with hEGF #8916 (100 ng/mL, 15 min; center), or treated with hEGF and λ phosphatase (right), using Phospho-p90RSK (Thr359) (D1E9) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from HeLa, L-929 and AR42J cells using RSK1/RSK2/RSK3 (32D7) #9355 Rabbit mAb.Western blot方法检测细胞提取物：HeLa, L-929，AR42J细胞，使用的抗体是RSK1/RSK2/RSK3 (32D7) #9355 Rabbit mAb。 | |
Western blot analysis of extracts from HeLa cells, untreated or treated with TPA #9905 (200 nM), using Phospho-p90RSK (Thr573) Antibody #9346 (upper) or RSK1/RSK2/RSK3 (32D7) Rabbit mAb #9355 (lower).Western blot方法检测细胞提取物：未经处理和TPA #9905(200 nM)处理的HeLa细胞，使用的抗体是Phospho-p90RSK (Thr573) Antibody #9346(上图)和RSK1/RSK2/RSK3 (32D7) Rabbit mAb #9355(下图)。 | |
Western blot analysis of extracts from HeLa and C6 cells, untreated or treated with TPA #9905 (200 nM), using Phospho-p90RSK (Thr359/Ser363) Antibody #9344 (upper) or control RSK1/RSK2/RSK3 Rabbit mAb #9355 (lower).Western blot方法检测细胞提取物：未经处理和TPA #9905(200 nM)处理的HeLa、C6 细胞，使用的抗体是Phospho-p90RSK (Thr359/Ser363) Antibody #9344 (上图)和control RSK1/RSK2/RSK3 Rabbit mAb #9355 (下图)。 | |
Western blot analysis of extracts from HeLa cells, untreated or TPA-treated (200 nM), using Phospho-p90RSK (Thr573) Antibody (upper) or RSK1/RSK2/RSK3 (32D7) Rabbit mAb #9355 (lower). |
