| 货号 | 9330T |
| 描述 | The FAK Antibody Sampler Kit provides an economical means of evaluating total FAK protein levels as well as FAK phosphorylated at specific sites. The kit contains enough primary and secondary antibody to perform four western blots with each antibody. |
| 目标/特异性 | Each antibody in the FAK Antibody Sampler Kit detects endogenous levels of its target protein and does not typically cross react with other proteins. Activation state antibodies recognize target proteins only when phosphorylated at the indicated residue. Phospho-FAK (Tyr397) Antibody may cross-react with overexpressed tyrosine phosphorylated EGF receptors. |
| 供应商 | CST |
| 背景 | Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration, and survival (1). Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for the Src family kinases PI3K and PLCγ (2-5). Recruitment of Src family kinases results in the phosphorylation of Tyr407, Tyr576, and Tyr577 in the catalytic domain, and Tyr871 and Tyr925 in the carboxy-terminal region of FAK (6,7). |
| 存放说明 | -20C |
| 参考文献 | Parsons, J.T. et al. (2000) Oncogene 19, 5606-13. Schaller, M.D. et al. (1994) Mol Cell Biol 14, 1680-8. Cobb, B.S. et al. (1994) Mol Cell Biol 14, 147-55. Chen, H.C. et al. (1996) J Biol Chem 271, 26329-34. Zhang, X. et al. (1999) Proc Natl Acad Sci U S A 96, 9021-6. Calalb, M.B. et al. (1995) Mol Cell Biol 15, 954-63. Schlaepfer, D.D. et al. (1994) Nature 372, 786-791. |
Western blot analysis of extracts from various cell lines using FAK (D2R2E) Rabbit mAb. | |
Immunoprecipitation of FAK from U-87 MG cell extracts using Rabbit (DA1E) mAb IgG XP®Isotype Control #3900 (lane 2) or FAK (D2R2E) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using FAK (D2R2E) Rabbit mAb. | |
Western blot analysis of extracts from A549 cells, untreated or treated with calf intestinal phosphatase (CIP), using Phospho-FAK (Tyr397) (D20B1) Rabbit mAb #8556 (upper) and FAK Antibody #3285 (lower). | |
Western blot analysis of extracts from A549 cells, untreated or treated with calf intestinal phosphatase (CIP), using Phospho-FAK (Tyr397) (D20B1) Rabbit mAb (upper) and FAK Antibody #3285 (lower). | |
Western blot analysis of extracts from various cell lines using FAK Antibody #3285.使用FAK抗体#3285对多种细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from Jurkat cells, untreated or treated with anti-CD3 antibody (1 µg/ml for 10 minutes), using Phospho-FAK (Tyr925) Antibody #3284 (upper) or FAK Antibody #3285 (lower).未处理或使用CD3抗体（1 µg/ml 10分钟 ）处理的Jurkat细胞提取物，使用Phospho-FAK (Tyr925) 抗体#3284（上）或FAK抗体#3285（下）进行western blot分析。 | |
Western blot analysis of extracts from Jurkat cells, untreated or treated with anti-CD3 antibody (1 µg/ml for 10 minutes), using Phospho-FAK (Tyr576/577) Antibody #3281 (upper) or FAK Antibody #3285 (lower).未处理或经过CD3抗体（1µg/ml 30分钟）处理的Jurkat细胞提取物使用 Phospho-FAK (Tyr576/577) 抗体#3281（上）或FAK抗体#3285（下）进行western blot分析。 | |
Western blot analysis of extracts from Jurkat cells, untreated or treated with anti-CD3 antibody (1 µg/ml for 10 minutes), using Phospho-FAK (Tyr576/577) Antibody (upper) or FAK antibody (lower). | |
Western blot analysis of extracts from Jurkat cells, untreated or anti-CD3 antibody-treated (1 µg/ml for 10 minutes), using Phospho-FAK (Tyr925) Antibody (upper) or FAK antibody (lower). | |
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition. |
