| 货号 | 9360T |
| 描述 | The PDGF Receptor α Antibody Sampler Kit provides an economical means of evaluating total PDGF receptor α protein (PDGFRα) levels as well as PDGFRα phosphorylated at specific sites. The kit contains enough primary and secondary antibody to perform four western blots with each antibody. |
| 目标/特异性 | Each antibody in the PDGF Receptor α Antibody Sampler Kit detects endogenous levels of its target protein, with activation state antibodies recognizing target proteins only when phosphorylated at the indicated residues. Phospho-PDGF Receptor α (Tyr849)/PDGF Receptor β (Tyr857) (C43E9) Rabbit mAb and Phospho-PDGF Receptor α (Tyr1018) Antibody may cross-react with other activated tyrosine kinases; PDGF Receptor α (D1E1E) XP® Rabbit mAb may cross-react with PDGFRβ at overexpressed levels. |
| 供应商 | CST |
| 背景 | The proteins of the platelet derived growth factor (PDGF) family exist as several disulphide-bonded, dimeric isoforms (PDGF AA, PDGF AB, PDGF BB, PDGF CC, and PDGF DD) that bind in a specific pattern to two closely related receptor tyrosine kinases, PDGF receptor α (PDGFRα) and PDGF receptor β (PDGFRβ). PDGFRα and PDGFRβ share 75% to 85% sequence homology between their two intracellular kinase domains while the kinase insert and carboxy-terminal tail regions display a lower level (27% to 28%) of homology (1). PDGFRα homodimers bind all PDGF isoforms except those containing PDGF D. PDGFRβ homodimers bind PDGF BB and DD isoforms, as well as the PDGF AB heterodimer. The heteromeric PDGF receptor α/β binds PDGF B, C, and D homodimers as well as the PDGF AB heterodimer (2). PDGFRα and PDGFRβ can each form heterodimers with EGFR, which is also activated by PDGF (3). Various cells differ in the total number of receptors present and in the receptor subunit composition, which may account for responsive differences among cell types to PDGF binding (4). Ligand binding induces receptor dimerization and autophosphorylation, followed by binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules such as Grb2, Src, GAP, PI3 kinase, PLCγ, and Nck. A number of different signaling pathways are initiated by activated PDGF receptors and lead to control of cell growth, actin reorganization, migration, and differentiation (5). Tyr751 in the kinase-insert region of PDGFRβ is the docking site for PI3 kinase (6). Phosphorylated pentapeptides derived from Tyr751 of PDGFRβ (pTyr751-Val-Pro-Met-Leu) inhibit the association of the carboxy-terminal SH2 domain of the p85 subunit of PI3 kinase with PDGFRβ (7). Tyr740 is also required for PDGFRβ-mediated PI3 kinase activation (8). |
| 存放说明 | -20C |
| 参考文献 | Deuel, T.F. et al. (1988) Biofactors 1, 213-217. Bergsten, E. et al. (2001) Nat. Cell Biol. 3, 512-516. Betsholtz, C. et al. (2001) Bioessays 23, 494-507. Coughlin, S.R. et al. (1988) Prog. Clin. Biol. Res. 266, 39-45. Ostman, A. and Heldin, C.H. (2001) Adv. Cancer Res. 80, 1-38. Panayotou, G. et al. (1992) EMBO J. 11, 4261-4272. Ramalingam, K. et al. (1995) Bioorg. Med. Chem. 3, 1263-1272. Kashishian, A. et al. (1992) EMBO J. 11, 1373-1382. |
Western blot analysis of extracts from NIH/3T3 and human skeletal muscle cells (SKMC), untreated or treated with PDGF-BB, using PDGF Receptor α (D1E1E) XP® Rabbit mAb #3174.用PDGF Receptor α (D1E1E) XP® Rabbit mAb #3174对NIH/3T3和human skeletal muscle cells (SKMC)细胞的提取物进行免疫印迹检测。 | |
Western blot analysis of extracts from NIH/3T3 cells, untreated or treated with PDGF AA, using Phospho-PDGF Receptor α (Tyr1018) Antibody #4547 (upper) or PDGF Receptor α Antibody #3164 (lower).用Phospho-PDGF Receptor α (Tyr1018) Antibody #4547（上）和PDGF Receptor α Antibody #3164 (下)对下列细胞提取物进行免疫印迹检测：经过或未经PDGF AA处理的NIH/3T3细胞的裂解液。 | |
Western blot analysis of NIH/3T3 cells, untreated or PDGF-treated, using Phospho-PDGF Receptor α (Tyr849)/PDGF Receptor β (Tyr857) (C43E9) Rabbit mAb # 3170 (upper) or PDGF Receptor β (28E1) Rabbit mAb #3169 (lower).用Phospho-PDGF Receptor α (Tyr849)/PDGF Receptor β (Tyr857) (C43E9) Rabbit mAb # 3170（上）和PDGF Receptor β (28E1) Rabbit mAb #3169 (下)对下列细胞提取物进行免疫印迹检测：经过或未经PDGF处理的NIH/3T3细胞的裂解液。 | |
Western blot analysis of extracts from various cell types expressing activated tyrosine kinase proteins using Phospho-PDGF Receptor α (Tyr754) (23B2) Rabbit mAb #2992 (upper) or Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 (lower). Phospho-PDGF Receptor α (Tyr754) (23B2) Rabbit mAb specifically binds to tyrosine phosphorylated PDGF receptor α, but not other phosphorylated tyrosine kinases.用Phospho-PDGF Receptor α (Tyr754) (23B2) Rabbit mAb #2992（上）和Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 (下) 对表达激活酪氨酸激酶的各种细胞的提取物进行免疫印迹检测。Phospho-PDGF Receptor α (Tyr754) (23B2) Rabbit mAb 特异地结合酪氨酸发生磷酸化的PDGF receptor ，但不结合其它磷酸化的酪氨酸激酶。 | |
Confocal immunofluorescent analysis of A-204 (left) and U-87 MG cells (right) using PDGF Receptor α (D1E1E) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of cell extracts from NIH/3T3 cells, untreated or treated with PDGF-AA, using Phospho-PDGF Receptor α (Tyr1018) Antibody (upper) or PDGF Receptor α Antibody #3164 (lower). | |
Immunohistochemical analysis of paraffin-embedded human colon using PDGR Receptor α (D1E1E) XP® Rabbit mAb. | |
Flow cytometric analysis of U-87 MG cells (blue) and H1703 cells (green) using PDGF Receptor α (D1E1E) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded HCC827 xenograft using PDGF Receptor α (D1E1E) XP® Rabbit mAb. | |
Western blot analysis of NIH/3T3 cells, untreated or treated with PDGFbb using Phospho-PDGF Receptor α (Tyr849)/PDGF Receptor β (Tyr857) (C43E9) Rabbit mAb (upper) and PDGF Receptor β (28E1) Rabbit mAb #3169 (lower). | |
Immunohistochemical analysis of paraffin-embedded U-118 MG xenograft using PDGF Receptor α (D1E1E) XP® Rabbit mAb in the presence of control peptide (left) or antigen specific peptide (right). | |
Immunohistochemical analysis of paraffin-embedded human glioblastoma using PDGR Receptor α (D1E1E) XP® Rabbit mAb. | |
Western blot analysis of extracts from NIH/3T3 and human skeletal muscle cells (SKMC), untreated or treated with PDGF-BB, using PDGF Receptor α (D1E1E) XP® Rabbit mAb. | |
Phospho-PDGF Receptor α (Tyr754) (23B2) Rabbit mAb specifically binds to tyrosine phosphorylated PDGF receptor α, but not other phosphorylated tyrosine kinases. Western blot analysis of of extracts from cells expressing different activated tyrosine kinase proteins, using Phospho-PDGF Receptor α (Tyr754) (23B2) Rabbit mAb (upper) or Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 (lower). | |
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition. |
