| 货号 | 9383T |
| 描述 | The TCF/LEF1 Antibody Sampler Kit provides an economical means of detecting total protein from the TCF/LEF1 family members. The kit contains enough primary and secondary antibodies to perform four Western blots with each antibody. |
| 目标/特异性 | Each antibody in this kit recognizes endogenous levels of total protein for the specified target and does not cross-react with other family members. TCF1 (C63D9) Rabbit mAb does not recognize the dominant negative isoforms of TCF1 lacking the amino-terminal β-catenin binding domain. LEF1 (C12A5) Rabbit mAb does not recognize the dominant negative forms of LEF1 generated by an alternative promoter. |
| 供应商 | CST |
| 背景 | LEF1 and TCF are members of the high mobility group (HMG) DNA binding protein family of transcription factors that consists of the following: Lymphoid Enhancer Factor 1 (LEF1), T Cell Factor 1 (TCF1), TCF3, and TCF4 (1). LEF1 and TCF1 were originally identified as important factors regulating early lymphoid development (2) and act downstream in Wnt signaling. LEF1 and TCF bind to Wnt response elements to provide docking sites for β-catenin, which translocates to the nucleus to promote the transcription of target genes upon activation of Wnt signaling (3). LEF1 and TCF are dynamically expressed during development and aberrant activation of the Wnt signaling pathway is involved in many types of cancers including colon cancer (4,5). |
| 存放说明 | -20C |
| 参考文献 | 1 . Waterman, M.L. (2004) Cancer Metastasis Rev 23, 41-52. 2 . Schilham, M.W. and Clevers, H. (1998) Semin Immunol 10, 127-32. 3 . Brantjes, H. et al. (2002) Biol Chem 383, 255-61. 4 . Reya, T. and Clevers, H. (2005) Nature 434, 843-50. 5 . Logan, C.Y. and Nusse, R. (2004) Annu Rev Cell Dev Biol 20, 781-810. |
Confocal immunofluorescent analysis of HCT-15 cells using LEF1 (C12A5) Rabbit mAb (green). Actin filaments have been labeled with DyLight™ 554 Phalloidin #13054 (red). | |
Western blot analysis of total cell lysates from HCT-15 and DLD-1 cells, and mouse thymocytes using LEF1 (C12A5) Rabbit mAb #2230.使用LEF1 (C12A5) 兔单抗#2230对 HCT-15 和 DLD-1细胞全细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from various cell types using TCF4 (C48H11) Rabbit mAb #2569.使用TCF4 (C48H11) 兔单抗#2569对多种细胞提取物进行western blot分析。 | |
Western blot analysis of total lysates from various cell lines using TCF3 (D15G11) XP® Rabbit mAb #2883.使用TCF3 (D15G11) XP® 兔单抗#2883对多种细胞全细胞提取物进行western blot分析。 | |
Western blot analysis of total cell lysates from HCT29, Colo201 and Jurkat cells, and mouse thymocytes using TCF1 (C63D9) Rabbit mAb #2203.使用TCF1 (C63D9)兔单抗#2203对HCT29, Colo201 和 Jurkat全细胞提取物进行western blot分析。 | |
Western blot analysis of total lysates from various cell lines using TCF3 (D15G11) Rabbit mAb. | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HCT 116 cells and either 10 μl of TCF4 (C48H11) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human CaMK2D Intron 3 Primers #5111, human c-Myc promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. | |
Immunohistochemical analysis of paraffin-embedded human Non-Hodgkins lymphoma using TCF1 (C63D9) Rabbit mAb. | |
Western blot analysis of extracts from various cell types using TCF4 (C48H11) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using TCF1 (C63D9) Rabbit mAb in the presence of control peptide (left) or TCF1 blocking peptide #1007 (right). | |
Immunohistochemical analysis of paraffin-embedded human tonsil using TCF1 (C63D9) Rabbit mAb. | |
Western blot analysis of total cell lysates from HCT15, DLD1 and mouse thymocytes using LEF1 (C12A5) Rabbit mAb. | |
Western blot analysis of total cell lysates from HCT29, Colo201, Jurkat and mouse thymocytes using TCF1 (C63D9) Rabbit mAb. | |
Confocal immunofluorescent analysis of DLD-1 cells using TCF1 (C63D9) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). | |
Flow cytometric analysis of Jurkat cells using TCF1 (C63D9) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). |
