| 货号 | 9615T |
| 描述 | The Phospho-(Ser) Kinase Substrate Antibody Sampler Kit provides a fast and economical means of evaluating several phospho-Ser kinase substrates. The kit contains enough primary and secondary antibody to perform four Western blot experiments. |
| 目标/特异性 | Each antibody detects endogenous levels of phospho-(Ser) proteins of specific kinase substrate groups. For more detailed information please see datasheet for this product. |
| 供应商 | CST |
| 背景 | Phospho-(Ser) kinases and phosphatases play critical roles in a wide range of biological processes. Each phospho-(Ser) kinase phosphorylates serine within a specific motif. The MAPK and CDK families of serine protein kinases phosphorylate serine followed by proline residue (1-3). The consensus amino acid sequence for CDK substrate is (K/R)(S*)PX(K/R), where X denotes any one of the 20 amino acids and S* is the phosphorylation site (4-6). MAPK phosphorylates substrates with the concensus sequence PX(S*)P. The 14-3-3 proteins are a highly conserved family of proteins involved in the regulation of cell survival, apoptosis, proliferation and checkpoint control (7-11). Binding of 14-3-3 is mediated through phospho-serine-containing proteins (12). Two different phospho-serine containing motifs are found using a degenerate phospho-serine-oriented peptide library technique, RSXS*XP and RXY/FXS*XP (12). Motif 1 (Arg/Lys and Ser at positions -3 and -2, phospho-Ser at position 0, and Pro at position +2) is found in critical regulatory proteins including Bad, cdc25C, FoxO3A, PKC and c-Raf (11, 13). Motif 2 (RXY/FXS*XP) is found in critical regulatory proteins including cdc25A, cdc25B, PKCγ, IRS-1 and BCR (12). Although Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody binds 14-3-3 binding motif 2 with no requirement for proline in the +2 position, it provides a powerful tool for the discovery and characterization of potential 14-3-3 binding motif 2-containing proteins or other proteins with the RXY/FXS* motif. Antibodies specific to particular kinase substrates are invaluable reagents in determining kinase activity and identifying potential new kinase substrates. CST has developed antibodies that recognize phosphorylated serine within the context of a protein motif that is phosphorylated by MAPK/CDK, CDKs or 14-3-3. As shown by DELFIA or ELISA, each phospho-(Ser) kinase substrate antibody in this sampler kit is specific to its kinase substrate motif. |
| 存放说明 | -20C |
| 参考文献 | 1 . Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81. 2 . Karin, M. (1994) Curr Opin Cell Biol 6, 415-24. 3 . Lewis, T. S. et al. (1998) Adv. Cancer Res. 74, 49-139. 4 . Songyang, Z. et al. (1996) Mol Cell Biol 16, 6486-93. 5 . Songyang, Z. (1999) Prog Biophys Mol Biol 71, 359-72. 6 . Holmes, J.K. and Solomon, M.J. (1996) J Biol Chem 271, 25240-6. 7 . Aitken, A. (1995) Trends Biochem Sci 20, 95-7. 8 . Zha, J. et al. (1996) Cell 87, 619-628. 9 . Piwnica-Worms, H. (1999) Nature 401, 535, 537. 10 . Tzivion, G. et al. (1998) Nature 394, 88-92. 11 . Xing, H. et al. (2000) EMBO J 19, 349-58. 12 . Muslin, A.J. et al. (1996) Cell 84, 889-97. 13 . Yaffe, M.B. et al. (1997) Cell 91, 961-71. |
对血清饥饿处理的A431细胞，未处理或EGF培养，以及Hela细胞，未处理或羟基脲处理(G1/S)或诺考达唑处理(G2/M)，抽提物使用Phospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb #2325进行Western blot分析。 | |
Western blot analysis of extracts from serum-starved A431 cells, untreated or treated in culture with EGF, and HeLa cells, untreated, hydroxyurea-treated (G1/S) or Nocodazole-treated (G2/M), using Phospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb #2325. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, control (left) or lambda phosphatase-treated (right), using Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb. 免疫组化分析石蜡包埋的人乳腺癌，使用的抗体为hospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb | |
免疫组化分析石蜡包埋的人肺癌，使用的抗体为hospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Phospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb. 免疫组化分析石蜡包埋的人结肠癌，使用的抗体为hospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb | |
Phospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb #2325 ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (S* denotes phosphorylated serine.) Phospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb #2325 ELISA分析：磷酸化和非磷酸化的信噪比。（S* 代表磷酸化的丝氨酸） | |
Western blot analysis of extracts from serum-starved A431 cells, untreated or treated in culture with EGF, and HeLa cells, untreated, hydroxyurea-treated (G1/S) or Nocodazole-treated (G2/M), using Phospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb. 对血清饥饿处理的A431细胞，未处理或EGF培养，以及Hela细胞，未处理或羟基脲处理(G1/S)或诺考达唑处理(G2/M)，抽提物使用Phospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb #2325进行Western blot分析。 | |
Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb ELISA assay: Signal-to-noise ratio of phospho- versus nonphospho-14-3-3 binding motif peptides. (S* denotes phosphorylated serine.) Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb ELISA分析：磷酸化和非磷酸化的信噪比。（S*代表磷酸化的丝氨酸） | |
Western blot analysis of extracts from A431 cells, untreated or calyculin A treated, using Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody. 对A431细胞，未处理或calyculin A处理，抽提物使用Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody进行免疫印迹分析 | |
Western blot analysis of extracts from A431 cells, untreated or calyculin A-treated, using Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb (left) or Phospho-(Ser) 14-3-3 Binding Motif Antibody #9601 (right). 对A431细胞，未处理或calyculin A处理，抽提物使用Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb (left) 或Phospho-(Ser) 14-3-3 Binding Motif Antibody #9601 (right)进行免疫印迹分析 | |
一抗结合靶蛋白后，加入二抗，HRP偶联的二抗复合物形成。然后加入LumiGLO*显色发光 |
