| 货号 | 9781T |
| 描述 | The Neurofilament Antibody Sampler Kit provides an economical means of evaluating neurofilaments. The kit contains enough primary and secondary antibodies to perform four western blot experiments per primary antibody. |
| 目标/特异性 | Neurofilament-L (DA2) Mouse mAb and Neurofilament-L (C28E10) Rabbit mAb detect endogenous levels of total Neurofilament-L protein. Neurofilament-M (RMO 14.9) Mouse mAb detects endogenous levels of total Neurofilament-M protein. Neurofilament-H (RMdO 20) Mouse mAb detects endogenous levels of total Neurofilament-H protein. Neurofilament-H (RMdO 20) Mouse mAb has been reported to detect NFM and NFH in human samples but only NFH in mouse, rat or bovine samples (Lee, V.M. et al., 1988). |
| 供应商 | CST |
| 背景 | The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Neurofilament accumulations are found in many human neurological disorders including Parkinsons disease (in Lewy bodies along with α-synuclein), Alzheimers disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1). |
| 存放说明 | -20C |
| 参考文献 | Al-Chalabi, A. and Miller, C.C. (2003) Bioessays 25, 346-55. Cohlberg, J.A. et al. (1995) J Biol Chem 270, 9334-9. Hisanaga, S. et al. (1994) Mol Biol Cell 5, 161-72. Sihag, R.K. et al. (1999) J Neurochem 72, 491-9. |
Immunohistochemical analysis of paraffin-embedded human brain using Neurofilament-L (DA2) Mouse mAb. | |
Western blot analysis of extracts from mouse brain, rat brain or CAD cells, using Neurofilament-H (RMdO 20) Mouse mAb #2836. | |
Western blot analysis of extracts from mouse brain, HeLa cells and rat brain, using Neurofilament-L (C28E10) Rabbit mAb #2837. | |
Western blot analysis of extracts from rat brain, mouse brain, U87-MG cells, C6 cells and human cerebellum, using Neurofilament-L (DA2) Mouse mAb #2835. | |
Western blot analysis of extracts from rat brain, untreated or treated with lambda phosphatase, using Neurofilament-M (RMO 14.9) Mouse mAb #2838. | |
Immunohistochemical analysis of paraffin-embedded mouse brain using Neurofilament-L (C28E10) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human brain using Neurofilament-L (C28E10) Rabbit mAb in the presence of control peptide (left) or Neurofilament-L blocking peptide #1005 (right). | |
Western blot analysis of extracts from mouse brain, HeLa cells and rat brain, using Neurofilament-L (C28E10) Rabbit mAb. | |
Confocal immunofluorescent analysis of normal rat cerebellum using Neurofilament-L (C28E10) Rabbit mAb (green) and GFAP (GA5) Mouse mAb #3670 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Confocal immunofluorescent image of mouse cerebellum labeled with Neurofilament-H (RMdO 20) Mouse mAb (green) and Calbindin Antibody #2136 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). | |
Western blot analysis of extracts from mouse brain, rat brain or CAD cells, using Neurofilament-H (RMdO 20) Mouse mAb. | |
Immunohistochemical analysis of paraffin-embedded human brain, using Neurofilament-H (RMdO 20) Mouse mAb. | |
Immunohistochemical analysis of paraffin-embedded human brain, using Neurofilament-M (RMO 14.9) Mouse mAb. | |
Western blot analysis of extracts from rat brain, untreated or treated with lambda phosphatase, using Neurofilament-M (RMO 14.9) Mouse mAb. | |
Confocal immunofluorescent image of mouse cerebellum labeled with Neurofilament-M (RMO 14.9) Mouse mAb (green) and Calbindin Antibody #2136 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). |
