| 货号 | 9971T |
| 描述 | The Toll-Like Receptor Antibody Sampler Kit is an economical way to examine the total protein levels of a number of toll-like receptors. This kit includes enough primary and secondary antibodies to perform four Western blot experiments with each antibody. |
| 目标/特异性 | All antibodies contained in this kit detect endogenous levels of their respective total TLR protein. Cross reactivity was not detected with other family members at physiological conditions. |
| 供应商 | CST |
| 背景 | Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-3). TLRs recognize conserved motifs found in various pathogens and mediate defense responses. Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes. The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the TIR domain. Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains including MyD88 (myeloid differentiation factor), MAL/TIRAP (MyD88-adaptor-like/TIR-associated protein), TRIF (Toll-receptor-associated activator of interferon), and TRAM (Toll-receptor-associated molecule). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK. Activation of IKK leads to the degradation of IκB that normally maintains NF-κB inactivity by sequestering it in the cytoplasm. |
| 存放说明 | -20C |
| 参考文献 | Akira, S. (2003) J Biol Chem 278, 38105-8. Beutler, B. (2004) Nature 430, 257-63. Dunne, A. and ONeill, L.A. (2003) Sci STKE 2003, re3. Medzhitov, R. et al. (1997) Nature 388, 394-7. Schwandner, R. et al. (1999) J Biol Chem 274, 17406-9. Takeuchi, O. et al. (1999) Immunity 11, 443-51. Alexopoulou, L. et al. (2001) Nature 413, 732-8. Zhang, F.X. et al. (1999) J Biol Chem 274, 7611-4. Horng, T. et al. (2001) Nat Immunol 2, 835-41. Oshiumi, H. et al. (2003) Nat Immunol 4, 161-7. Muzio, M. et al. (1997) Science 278, 1612-5. Wesche, H. et al. (1997) Immunity 7, 837-47. Suzuki, N. et al. (2002) Nature 416, 750-6. Irie, T. et al. (2000) FEBS Lett 467, 160-4. |
Immunoprecipitation of TLR2 from MUTZ-3 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Toll-like Receptor 2 (D7G9Z) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Toll-like Receptor 2 (D7G9Z) Rabbit mAb. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing HA-tagged full-length human TLR2 (hTLR2-HA; +), using Toll-like Receptor 2 (D7G9Z) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using Toll-like Receptor 9 (D2C9) Rabbit mAb. | |
Western blot analysis of THP-1 and MUTZ-3 cells using Toll-like Receptor 2 (D7G9Z) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using Toll-like Receptor 7 (D7) Rabbit mAb. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with human TLR7 (+), using Toll-like Receptor 7 (D7) Rabbit mAb. | |
Western blot analysis of extracts from Raji and Ramos cells using Toll-like Receptor 9 Antibody #2254.Western免疫印迹。用Toll-like Receptor9 Antibody #2254研究Raji 和Ramos 细胞的细胞提取液。 | |
Western blot analysis of extracts from HeLa cells, mock transfected or transfected with human TLR7, and from Ramos and A20 cell lines using Toll-like Receptor 7 Antibody #2633.Western免疫印迹。用Toll-like Receptor 7 Antibody #2633研究对照转染和转染了人TLR7的HeLa细胞的细胞提取液。 | |
Western blot analysis of extracts from THP-1 cell lines, stimulated with 200 µM TPA overnight, using Toll-like Receptor 2 Antibody #2229.Western免疫印迹。用Toll-like Receptor 2 Antibody #2229研究经200 µM TPA 刺激过夜的THP-1细胞系的细胞提取液。 | |
Western blot analysis of extracts from SR cells using Toll-like Receptor 1 Antibody #2209.Western免疫印迹。用Toll-like Receptor 1 Antibody #2209研究SR细胞的细胞提取液。 | |
Western blot analysis of extracts from SR cells using Toll-like Receptor 1 Antibody. | |
Western blot analysis of extracts from HeLa cells, mock transfected or transfected with human TLR1, using Toll-like Receptor 1 Antibody. | |
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition. |
