| 货号 | 9780T |
| 描述 | The PP2A Antibody Sampler Kit provides an economical means of evaluating PP2A protein. The kit contains enough primary and secondary antibodies to perform four western blots with each antibody.PP2A Antibody Sampler Kit为研究PP2A提供了一个经济的方式。该试剂盒提供了足够4次western blot实验的一抗和二抗。 |
| 目标/特异性 | PP2A A Subunit (81G5) Rabbit mAb detects endogenous levels of α and β isoforms of the PP2A A subunit and does not cross-react with other PP2A subunits. PP2A B Subunit (100C1) Rabbit mAb detects endogenous levels of the α isoform of PP2A B and may recognize the β, γ, and δ isoforms of PP2A B. The antibody does not cross-react with the B, B or B families of PP2A B subunits. PP2A C Subunit (52F8) Rabbit mAb detects endogenous levels of α and β isoforms of the PP2A catalytic subunit and does not cross-react with other PP2A subunits. |
| 供应商 | CST |
| 背景 | Protein phosphatase type 2A (PP2A) is an essential protein serine/threonine phosphatase that is conserved in all eukaryotes. PP2A is a key enzyme within various signal transduction pathways as it regulates fundamental cellular activities such as DNA replication, transcription, translation, metabolism, cell cycle progression, cell division, apoptosis and development (1-3). The core enzyme consists of catalytic C and regulatory A (or PR65) subunits, with each subunit represented by α and β isoforms (1). Additional regulatory subunits belong to four different families of unrelated proteins. Both the B (or PR55) and B regulatory protein families contain α, β, γ and δ isoforms, with the B family also including an ε protein. B family proteins include PR72, PR130, PR59 and PR48 isoforms, while striatin (PR110) and SG2NA (PR93) are both members of the B regulatory protein family. These B subunits competitively bind to a shared binding site on the core A subunit (1). This variable array of holoenzyme components, particularly regulatory B subunits, allows PP2A to act in a diverse set of functions. PP2A function is regulated by expression, localization, holoenzyme composition and post-translational modification. Phosphorylation of PP2A at Tyr307 by Src occurs in response to EGF or insulin and results in a substantial reduction of PP2A activity (4). Reversible methylation on the carboxyl group of Leu309 of PP2A has been observed (5,6). Methylation alters the conformation of PP2A, as well as its localization and association with B regulatory subunits (6-8).蛋白磷酸酶2A (PP2A)是一种重要的蛋白丝氨酸/苏氨酸磷酸酶，在所有的真核细胞中结构保守。PP2A在各种细胞信号转导通路中都是关键的酶，它调节基本的细胞活动如DNA复制、转录、翻译、代谢、细胞周期进程、细胞分化、细胞凋亡和发育(1-3)。核心的酶由催化性的C亚基和调节性的A亚基(PR65)组成，每个亚基都包括α 和β异构体(1)。另外的亚基属于四个不相关蛋白的不同家族。B (PR55)和B调节蛋白家族都包含α，β，γ 和δ异构体，而B还含有ε蛋白。B家族包括PR72，PR130，PR59 和PR48异构体，而纹蛋白(PR110)和SG2NA (PR93)都属于B调节蛋白家族。这些B亚基竞争性的结合核心亚基A上面的一个共同结合位点(1)。该全酶的可变阵列，尤其是调节性B亚基，允许PP2A具有多种功能。PP2A的功能受其表达、分布、全酶构成和转录后修饰的调控。Src介导的PP2A第307位酪氨酸磷酸化响应EGF或者胰岛素，最终导致PP2A的活性显著下降(4)。已观察到PP2A的第309位亮氨酸的羧基官能团可发生可逆的甲基化修饰(5,6)。甲基化改变了PP2A的构象，也改变了它的定位和B调节亚基的相互作用(6-8)。 |
| 存放说明 | -20C |
| 参考文献 | Janssens, V. and Goris, J. (2001) Biochem J 353, 417-39. Zolnierowicz, S. (2000) Biochem Pharmacol 60, 1225-35. Millward, T.A. et al. (1999) Trends Biochem Sci 24, 186-91. Chen, J. et al. (1992) Science 257, 1261-4. Turowski, P. et al. (1995) J Cell Biol 129, 397-410. Lee, J. et al. (1996) Proc Natl Acad Sci U S A 93, 6043-7. Tolstykh, T. et al. (2000) EMBO J 19, 5682-91. Yu, X.X. et al. (2001) Mol Biol Cell 12, 185-99. |
Western blot analysis of extracts from various cell lines using PP2A B Subunit (100C1) Rabbit mAb #2290. Western blot方法检测不同细胞提取物，使用的抗体为PP2A B Subunit (100C1) Rabbit mAb #2290。 | |
Western blot analysis of extracts from various cell lines using PP2A C Subunit (52F8) Rabbit mAb #2259.Western blot 方法检测不同细胞提取物，使用的抗体PP2A C Subunit (52F8) Rabbit mAb #2259。 | |
Western blot analysis of extracts from various cell lines using PP2A A Subunit (81G5) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using PP2A A Subunit (81G5) Rabbit mAb #2041..Western blot 方法检测不同细胞提取物，使用的抗体PP2A A Subunit (81G5) Rabbit mAb #2041。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PP2A A Subunit (81G5) Rabbit mAb. | |
Confocal immunofluorescent analysis of HeLa cells using PP2A A Subunit (81G5) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using PP2A B Subunit (100C1) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PP2A C Subunit (52F8D8) Rabbit mAb. | |
Western blot analysis of cell lysates from HeLa, NIH/3T3, C6 and COS cells, using PP2A B Subunit (100C1) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using PP2A C Subunit (52F8D8) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using PP2A C Subunit (52F8D8) Rabbit mAb in the presence of an irrelevant control peptide (left) or PP2A C Subunit Blocking Peptide #1067 (right). | |
Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells, using PP2A C (52F8D8) Rabbit mAb. | |
Flow cytometric analysis of HeLa cells, using PP2A C Subunit (52F8D8) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). | |
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition. |
