| 货号 | 9094T |
| 描述 | The StemLight™ Surface Marker Kit contains a panel of antibodies for the detection of antigens that are specifically expressed on the surface of human pluripotent cells. The kit can be used to track the pluripotent potential of human embryonic stem (ES) or induced pluripotent (iPS) cells. The loss of these markers indicates a loss of pluripotency or differentiation of the culture. The kit components are pre-optimized for parallel use in immunofluorescent analysis. |
| 目标/特异性 | TRA-1-60(S) (TRA-1-60(S)) Mouse mAb detects endogenous levels of the neuraminidase-sensitive TRA-1-60 antigen. TRA-1-81 (TRA-1-81) Mouse mAb detects endogenous levels of TRA-1-81 antigen. SSEA4 (MC813) Mouse mAb detects endogenous levels of SSEA4 antigen. |
| 供应商 | CST |
| 背景 | Pluripotency is the ability of a cell to differentiate into cell types of the three germ layers, the endoderm, ectoderm, and mesoderm. It is a property shared by embryonic stem cells, embryonic carcinoma, and induced pluripotent cells. SSEA4, TRA-1-81, and TRA-1-60 antibodies recognize antigens expressed on the cell surface of all pluripotent cells. SSEA4 recognizes a glycolipid carbohydrate epitope (1). TRA-1-60(S) and TRA-1-81 antibodies recognize different proteoglycan epitopes on variants of the same protein, podocalyxin (2). These epitopes are neuraminidase sensitive and resistant, respectively. Reactivity of SSEA4, TRA-1-81 and TRA-1-60 antibodies with their respective cell surface markers are lost upon differentiation of pluripotent cells, corresponding with a loss of pluripotent potential (3). |
| 存放说明 | -20C |
| 参考文献 | Henderson, J.K. et al. (2002) Stem Cells 20, 329-37. Draper, J.S. et al. (2002) J Anat 200, 249-58. Schopperle, W.M. and DeWolf, W.C. (2007) Stem Cells 25, 723-30. |
Flow cytometric analysis of unpermeabilized Jurkat cells (blue) and unpermeabilized NTERA cells (green) using SSEA4 (MC813) Mouse mAb. | |
Confocal immunofluorescent analysis of NTERA-2 cells, using TRA-1-60(S) (TRA-1-60(S)) Mouse mAb, TRA-1-81 (TRA-1-81) Mouse mAb, and SSEA4 (MC813) Mouse mAb. Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用 TRA-1-60(S) (TRA-1-60(S)) 鼠单抗, TRA-1-81 (TRA-1-81) 鼠单抗,和SSEA4 (MC813) 鼠单抗对NTERA-2细胞进行激光共聚焦荧光分析。使用DY-554鬼笔环肽（红色）标记肌动蛋白丝。蓝色假色=DRAQ5#4084（DNA荧光染料） | |
Immunohistochemical analysis of paraffin-embedded NTERA (positive, left) and Jurkat (negative, right) cell pellets using TRA-1-81 (TRA-1-81) Mouse mAb. | |
Immunohistochemical analysis of paraffin-embedded NTERA (positive, left) and Jurkat (negative, right) cell pellets using TRA-1-60(S) (TRA-1-60(S)) Mouse mAb. | |
Confocal immunofluorescent analysis of NTERA2 cells using SSEA4 (MC813) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). | |
Flow cytometric analysis of unpermeabilized Jurkat cells (blue) and unpermeabilized NCCIT cells (green) using TRA-1-81 (TRA-1-81) Mouse mAb. | |
Western blot analysis of extracts from various cell lines using TRA-1-60 (TRA-1-60) Mouse mAb. | |
Flow cytometric analysis of unpermeabilized Jurkat cells (blue) and unpermeabilized NTERA2 cells (green) using TRA-1-60(S) (TRA-1-60(S)) Mouse mAb. | |
Confocal immunofluorescent analysis of nTERA2 cells using TRA-1-60(S) (TRA-1-60(S)) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Confocal immunofluorescent analysis of NTERA-2 cells using TRA-1-81 (TRA-1-81) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
