| 货号 | 9329T |
| 目标/特异性 | Each antibody in the MAPKAPK-2 Antibody Sampler Kit detects endogenous levels of its target protein. Activation state antibodies detect only target proteins phosphorylated at indicated residues. MAPKAPK-2 (D1E11) Rabbit mAb is not known to cross-react with other MAPKAPK proteins. |
| 供应商 | CST |
| 背景 | In response to cytokines, stress and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK (1). Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272 and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay (2). Phosphorylation at Thr222, Ser272 and Thr334 appears to be essential for the activity of MAPKAPK-2 (2). Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2 (2). |
| 存放说明 | -20C |
| 参考文献 | Rouse, J. et al. (1994) Cell 78, 1027-1037. Ben-Levy, R. et al. (1995) EMBO J. 14, 5920-5930. |
Western blot analysis of cell extracts from MAPKAPK-2 wild type (WT) and knockout (KO) mouse embryonic fibroblasts (MEF) using MAPKAPK-2 (D1E11) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). MAPKAPK-2 WT and KO MEFs were generously provided by Drs. Sandra Morandell and Michael Yaffe, David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA. | |
Immunoprecipitation of MAPKAPK-2 from HeLa cell extracts using MAPKAPK-2 (D1E11) Rabbit mAb (lane 2), MAPKAPK-2 Antibody #3042 (lane 3), or Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 4). Lane 1 is 10% input. Western blot analysis was performed using MAPKAPK-2 (D1E11) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody. | |
Western blot analysis of extracts from various cell lines, untreated (-) or treated with UV (40 mJ/cm2, 30 min recovery; +), using MAPKAPK-2 (D1E11) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). | |
Western blot analysis of extracts from NIH/3T3 cells, untreated or UV and TPA-treated, using Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb #3316 (upper) and MAPKAPK-2 Antibody #3042 (lower).Western blot方法检测细胞提取物：未经处理的和紫外照射、TPA处理的NIH/3T3细胞，使用的抗体是Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb #3316 (上图) 和MAPKAPK-2 Antibody #3042 (下图)。 | |
Western blot analysis of extracts from HeLa and COS cells, untreated or UV and TPA-treated, using Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb #3007 (upper) and MAPKAPK-2 Antibody #3042 (lower).Western blot方法检测细胞提取物：未经处理的和紫外照射、TPA处理的HeLa、COS 细胞，使用的抗体是Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb #3007 (上图)和MAPKAPK-2 Antibody #3042 (下图)。 | |
Western blot analysis of extracts from HeLa and COS cells, untreated or UV-treated, using MAPKAPK-2 Antibody #3042.Western blot方法检测细胞提取物：未经处理的和紫外照射的HeLa、COS 细胞，使用的抗体是MAPKAPK-2 Antibody #3042。 | |
Confocal immunofluorescent analysis of HeLa cells, untreated (left), UV-treated (center) or UV and lambda phosphatase-treated (right), using Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). | |
Western blot analysis of extracts from HeLa cells, untreated (-) or UV- and TPA-treated (+), using Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb (upper), or MAPKAPK-2 Antibody #3042 (lower). | |
Flow cytometric analysis of HeLa cells, untreated (blue) or UV-treated (green), using Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb. | |
Western blot analysis of extracts from untreated or UV+TPA-treated HeLa and COS cells, using Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb (upper), or MAPKAPK-2 Antibody #3042 (lower). | |
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition. |
