c-Kit Antibody Sampler Kit【科瑞奥博】

产品中心

当前位置：首页>产品中心

c-Kit Antibody Sampler Kit

货号： 9370T 基本售价： 3216.0 元规格： -

产品信息

概述
货号	9370T
描述	The c-Kit Antibody Sampler Kit provides a fast and economical means of evaluating levels of c-Kit receptor protein phosphorylated at the specified sites, as well as total c-Kit receptor levels. The kit contains enough primary and secondary antibody to perform four Western blot experiments.
目标/特异性	Phospho-c-Kit antibodies detect endogenous levels of c-Kit when phosphorylated at the specified sites. c-Kit (D13A2) XP® Rabbit mAb detects endogenous levels of total c-kit receptor protein.

性能
供应商	CST
背景	c-Kit is a member of the subfamily of receptor tyrosine kinases that includes PDGF, CSF-1, and FLT3/flk-2 receptors (1,2). It plays a critical role in activation and growth in a number of cell types including hematopoietic stem cells, mast cells, melanocytes, and germ cells (3). Upon binding with its stem cell factor (SCF) ligand, c-Kit undergoes dimerization/oligomerization and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components including PLCγ, the p85 subunit of PI3 kinase, SHP2, and CrkL (4). Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders (5), and mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors (6). Tyr719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo (7).
存放说明	-20C
参考文献	Martin, F.H. et al. (1990) Cell 63, 203-11. Yarden, Y. et al. (1987) EMBO J 6, 3341-51. Gommerman, J.L. et al. (1997) J Biol Chem 272, 30519-25. Sattler, M. et al. (1997) J Biol Chem 272, 10248-53. Nocka, K. et al. (1990) EMBO J 9, 1805-13. Hirota, S. et al. (1998) Science 279, 577-80. Blume-Jensen, P. et al. (2000) Nat Genet 24, 157-62.

参考图片

Confocal immunofluorescent analysis of NCI-H526 (left) and Jurkat (right) cells using c-Kit (D13A2) XP^® Rabbit mAb (green). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Western blot analysis of extracts from H526 cells, untreated or SCF #9907 treated (50 ng/ml for 2 minutes) using Phospho-c-Kit (Tyr703) (D12E12) XP® Rabbit mAb #3073, Phospho-c-Kit (Tyr719) Antibody #3391, or c-Kit (D13A2) XP® Rabbit mAb #3074.用Phospho-c-Kit (Tyr703) (D12E12) XP® Rabbit mAb #3073、Phospho-c-Kit (Tyr719) Antibody #3391或c-Kit (D13A2) XP® Rabbit mAb #3074对经过SCF #9907 (50 ng/ml， 2 分钟)处理的H526细胞进行免疫印迹检测。

Western blot analysis of extracts of H526 cells, untreated or treated with SCF, using Phospho-c-Kit (Tyr703) (D12E12) Rabbit mAb (upper) or c-Kit Antibody #3392 (lower).

Western blot analysis of extracts from NCI-H526 cells using c-Kit (D13A2) XP^® Rabbit mAb.

Western blot analysis of extracts from MO7e cells, untreated or stimulated with SCF #9907 (50 ng/ml for 2 minutes), using Phospho-c-Kit (Tyr719) Antibody (upper) or c-Kit Antibody #3392 (lower).

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.