| 货号 | 9555T |
| 描述 | The cdc25C Antibody Sampler Kit provides an economical means to investigate the entry of eukaryotic cells into mitosis. The kit contains enough primary and secondary antibodies to perform four Western blots with each antibody.cdc25C Antibody Sampler Kit提供了一个检测真核细胞进入有丝分裂期的经济适用的方法。本试剂盒中的每个抗体都有足够4次western blot检测用的一抗和二抗。 |
| 目标/特异性 | Each antibody in the cdc25C Antibody Sampler Kit detects endogenous levels of its respective target protein and does not cross-react with other family members. |
| 供应商 | CST |
| 背景 | cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5).cdc25蛋白磷酸酶负责cdc2的去磷酸化和激活，该过程是真核细胞进入有丝分裂的关键调节步骤(1)。整个分裂间期cdc25C丝氨酸216位发生c-TAK1引起的组成性磷酸化，而该位点的磷酸化在G2/M期检验点是不依赖DNA损伤的(2)。当216位丝氨酸磷酸化时，cdc25C结合14-3-3蛋白家族的成员，扣押胞质cdc25C从而阻止有丝分裂的过早发生(3)。检验点蛋白激酶Chk1和Chk2磷酸化cdc25C216位丝氨酸以响应DNA损伤(4,5)。 |
| 存放说明 | -20C |
| 参考文献 | Jessus, C. and Ozon, R. (1995) Prog. Cell Cycle Res. 1, 215-228. Peng, C.Y. et al. (1997) Science 277, 1501-1505. Kumagai, A. and Dunphy, W.G. (1999) Genes Dev. 13, 1067-1072. Blasina, A. et al. (1999) Curr. Biol. 9, 1-10. Furnari, B. et al. (1999) Mol. Biol. Cell 10, 833-845. Izumi, T. and Maller, J.L. (1993) Mol Biol Cell 4, 1337-50. Stukenberg, P.T. and Kirschner, M.W. (2001) Mol Cell 7, 1071-83. Yaffe, M.B. et al. (1997) Science 278, 1957-60. Lu, P.J. et al. (1999) Science 283, 1325-8. Landrieu, I. et al. (2001) J Biol Chem 276, 1434-8. |
Western blot analysis of extracts from HT-29 cells, untreated, nocodazole-treated or λ phosphatase-treated, using Phospho-cdc25C (Ser216) Rabbit mAb #4901 (upper) and cdc25C (5H9) Rabbit mAb #4688 (lower). western blot方法检测未处理、nocodazole处理和λ磷酸酶处理的HT-29细胞提取物，使用的抗体为Phospho-cdc25C (Ser216) Rabbit mAb #4901 (上图)和 cdc25C (5H9) Rabbit mAb #4688 (下图). | |
Western blot analysis of extracts from HeLa, 293 and K-562 cells using cdc25C (5H9) Rabbit mAb #4688.western blot方法检测HeLa、293和K-562细胞提取物，使用 cdc25C (5H9) Rabbit mAb #4688 | |
Western blot analysis of extracts from HT-29 cells, untreated, nocodazole-treated or λ phosphatase-treated, using Phospho-cdc25C (Thr48) #9527 (upper) and cdc25C (5H9) Rabbit mAb #4688 (lower). western blot方法检测未处理、nocodazole处理和λ磷酸酶处理的HT-29细胞提取物，使用的抗体为 Phospho-cdc25C (Thr48) #9527 (上图) 和 cdc25C (5H9) Rabbit mAb #4688 (下图). | |
Western blot analysis of HT29 cell extracts untreated, nocodazole-treated, and λ phosphotase-treated using Phospho-cdc25C (Thr48), #9527 (upper), and cdc25C (5H9) Rabbit mAb (lower). | |
Western Blot analysis of HT29 cell extracts untreated, nocodazole-treated and lambda phosphotase-treated using Phospho-cdc25C (Thr48) (upper), and cdc25C (5H9) Rabbit mAb, #4688 (lower). | |
Western Blot analysis of HT29 cells, untreated, nocodazole-treated, and lambda phosphotase-treated, using Phospho-cdc25C (Ser216) antibody (upper), and cdc25C (5H9) Rabbit Mab, #4688, (lower). | |
Western blot analysis of extracts from HeLa, 293 and K562 cells, using cdc25C (5H9) Rabbit mAb. | |
Western blot analysis of GST-cdc25C, unphosphorylated or phosphorylated in vitro by Chk2, using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb (upper) or cdc25C antibody #9522 (lower). | |
Immunohistochemical analysis of paraffin-embedded human lymph node, using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb in the presence of control peptide (left) or Phospho-cdc25C (Ser216) Blocking Peptide #1190 (right). | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, untreated (left) or lambda phosphatase-treated (right), using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing cytoplasmic localization, using Phospho-cdc25C (Ser216) (63F9) Rabbit mAb. | |
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition. |
