| 货号 | 9776T |
| 描述 | The Myosin Light Chain 2 Antibody Sampler Kit provides an economical means to detect total, phosphorylated, and dual-phosphorylated myosin light chain 2. The kit contains enough primary and secondary antibody to perform four western blot experiments. |
| 目标/特异性 | The antibodies in the Myosin Light Chain 2 Antibody Sampler Kit detect endogenous levels of total myosin light chain 2 (smooth muscle), myosin light chain 2 when phosphorylated at Ser19 or when dually phosphorylated at Thr18 and Ser19, respectively. The antibodies do not cross-react with the cardiac isoform of Myosin Light Chain 2. |
| 供应商 | CST |
| 背景 | Myosin is composed of six polypeptide chains: two identical heavy chains and two pairs of light chains. Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC or RLC, LC20), has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+/calmodulin-dependent manner (1). This phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction (2). ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fibers (3). Phosphorylation of smooth muscle MLC2 at Ser1/Ser2 and Ser9 by PKC and cdc2 has been reported to inhibit myosin ATPase activity (4,5). Phosphorylation by cdc2 controls the timing of cytokinesis (5). Transgenic mice lacking phosphorylation sites on the cardiac muscle isoform show morphological and functional abnormalities (6). |
| 存放说明 | -20C |
| 参考文献 | Ikebe, M. and Hartshorne, D.J. (1985) J. Biol. Chem. 260, 10027-10031. Tan, J. L. et al. (1992) Annu. Rev. Biochem. 61, 721-759. Totsukawa, G. et al. (2000) J. Cell Biol. 150, 797-806. Ikebe, M. et al. (2000) J. Biol. Chem. 262, 9569-9573. Satterwhite, L. L. et al. (1992) J. Cell Biol. 118, 595-605. Sanbe, A. et al. (1999) J. Biol. Chem. 274, 21085-21094. |
Western blot analysis of extracts from various cell lines using Myosin Light Chain 2 (D18E2) Rabbit mAb. | |
Western blot analysis of extracts from 293 cells stimulated with ionophore A23187 for the indicated times using Phospho-Myosin Light Chain 2 (Thr18/Ser19) Antibody #3674. | |
Western blot analysis of extracts from 293 cells stimulated with ionophore A23187 for the indicated times, using Phospho-Myosin Light Chain 2 (Ser19) Antibody #3671. | |
Western blot analysis of extracts from 293, PL45, A431, C2C12 and C6 cells using Myosin Light Chain 2 Antibody #3672. | |
Confocal immunofluorescent images of HeLa cells, untreated (left) or phosphatase-treated (right), labeled with Phospho-Myosin Light Chain 2 (Ser19) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from HEK293 cells stimulated with ionophore A23187 for the indicated times, using Phospho-Myosin Light Chain 2 (Thr18/Ser19) Antibody. | |
Western blot analysis of extracts from HEK293 cells stimulated with ionophore A23187 for the indicated times, using Phospho-Myosin Light Chain 2 (Ser19) Antibody. | |
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition. |
