| 货号 | 9793T |
| 目标/特异性 | SHP-2 (D50F2) Rabbit mAb detects endogenous levels of total SHP-2 protein. Phospho- (Tyr542) SHP-2 Antibody detects endogenous SHP-2 only when phosphorylated at the specified at Tyr542. Phospho- SHP-2 (Tyr580) Antibody detects endogenous level of SHP2 only when phosphorylated at Tyr580. Some cross-reactivity with phosphorylated RTKs may occur. See individual product webpages for more information. |
| 供应商 | CST |
| 背景 | SHP-2 (PTPN11) is a ubiquitously expressed, nonreceptor protein tyrosine phosphatase (PTP). It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens, and extracellular matrices in the control of cell growth, differentiation, migration, and death (1). Activation of SHP-2 and its association with Gab1 is critical for sustained Erk activation downstream of several growth factor receptors and cytokines (2). In addition to its role in Gab1-mediated Erk activation, SHP-2 attenuates EGF-dependent PI3 kinase activation by dephosphorylating Gab1 p85 binding sites (3). SHP-2 becomes phosphorylated at Tyr542 and Tyr580 in its carboxy-terminus in response to growth factor receptor activation (4). These phosphorylation events are thought to relieve basal inhibition and stimulate SHP-2 tyrosine phosphatase activity (5). Mutations in the corresponding gene result in a pair of clinically similar disorders (Noonan syndrome and LEOPARD syndrome) that may result from abnormal MAPK regulation (6). |
| 存放说明 | -20C |
| 参考文献 | Qu, C.K. (2000) Cell Res 10, 279-88. Maroun, C.R. et al. (2000) Mol Cell Biol 20, 8513-25. Zhang, S.Q. et al. (2002) Mol Cell Biol 22, 4062-72. Bennett, A.M. et al. (1994) Proc Natl Acad Sci USA 91, 7335-9. Lu, W. et al. (2001) Mol Cell 8, 759-69. Edouard, T. et al. (2007) Cell Mol Life Sci 64, 1585-90. |
Western blot analysis of extracts from C6 and NIH/3T3 cells, starved for 18 hours and either untreated or PDGF-treated (50ng/ml, 20 minutes), using Phospho-SHP-2 (Tyr542) Antibody #3751 (upper) or total SHP-2 Antibody #3752 (lower). | |
Western blot analysis of total cell lysates from NIH/3T3 cells, serum-starved overnight and treated with PDGF (#9909) for 5 minutes, using Phospho-SHP-2 (Tyr580) Antibody #3703 (upper) or control SHP-2 Antibody #3752 (lower). | |
Western blot analysis of extracts from various cell lines using SHP-2 (D50F2) Rabbit mAb #3397. | |
Western blot analysis of extracts from various cell lines using SHP-2 (D50F2) Rabbit mAb. | |
Western blot analysis of total cell lysates from NIH/3T3 cells, serum-starved overnight and treated with PDGF (#9909) for 5 minutes, using Phospho-SHP-2 (Tyr580) Antibody (upper) or control SHP-2 Antibody #3752 (lower). | |
Western blot analysis of extracts from C6 and NIH/3T3 cells, starved for 18 hours and either untreated or PDGF-treated (50ng/ml, 20 minutes), using Phospho-SHP-2 (Tyr542) Antibody (upper) or control SHP-2 Antibody #3752 (lower). | |
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition. |
