Mouse Apoptosis Detection Antibody Pack
货号：
NBP2-25080 基本售价：
3162.7 元 规格：
-
产品信息
概述| 货号 | NBP2-25080 |
| 来源宿主 | Mouse |
| 应用 | WB |
| 目标/特异性 | The Apoptosis Detection Antbody Bundle contains monoclonal antibodies to three key apoptosis markers: caspase-3, caspase-8, and cleaved PARP. The caspase-3 and -8 antibodies both recognize pro and cleaved forms. The PARP antibody specifically recognizes the 89 kDa PARP fragment and does not recognized the full-length protein. |
性能| 供应商 | Novus |
| 免疫原 | The following antibodies are provided in Apoptosis Detection Antibody Bundle: 1. Caspase-3 (Pro and Active/Cleaved): 0.025mg: Localized to the large domain subunit: WB, IHC (paraffin), IHC (frozen): H, M, R: Western blot: pro and active forms seen2. Caspase-8 (Pro and Active/Cleaved): 0.025mg: 370 PVETDSEEQP 379: WB: H, Ch, Rh: Western blot: pro and active forms seen3. PARP (cleavage-specific): 0.025mg: A peptide containing amino acids near the 214/215-cleavage site: WB, Flow: Human: Western blot: specifically detects the cleaved form of PARP |
| 背景 | Apoptosis, a form of programmed cell death, is among the most highly studied and analyzed processes in cell biology. The components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery and have central roles in the initiation and execution of apoptosis.Caspases are synthesized as inactive pro enzyme precursors (zymogens) of ~30-55 kDa with an N-terminal prodomain of variable length followed by a large subunit (p20) and a small subunit (p10). Caspase activation is a hallmark of apoptotic signaling pathways. Caspases are activated early during apoptosis through proteolytic cleavage at specific asparagine residues by self-proteolysis and/or cleavage by other caspases. The asparagine resides are located within the prodomain, the p10, and p20 subunits. Activation results in the generation of mature active caspases consisting of the heterotetramer p202-p102. The cleavage forms present are markers of caspase-dependent apoptosis. The reduction or loss of caspase proforms can also be a marker of caspase-dependent apoptosis.Caspase activation is followed by cleavage of a myriad of caspase substrates which contributes to the demise and eventual death of the cell. The most highly characterized caspase substrate is PARP. PARP [(ADP-ribose) polymerase] is a 113 kDa nuclear chromatin-associated enzyme that is involved in DNA repair and other cellular events. The cleavage of PARP into an 89 and 28 kDa fragment is considered indicative of functional caspase activation. |
| 存放说明 | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
参考图片Western Blot: Apoptosis Detection Antibody Pack [NBP2-25080] - Analysis of cleaved PARP in staurosporin-treated Jurkat cells at various time points, using at 2 ug/ml. The band corresponding to cleaved PARP is only seen in the treated samples. anti-mouse Ig HRP conjugate was used in this test. |
Western Blot: Apoptosis Detection Antibody Pack [NBP2-25080] - Analysis of Caspase-3 in Jurkat cells using this antibody. Cells were treated with 2 uM staurosporine for different time periods. Caspase-3 activation is detected in western blot by the presence of Caspase-3 cleavage fragments. this antibody. A basal level of endogenous active Caspase-3 may be see in untreated Jurkat cells. anti-mouse Ig HRP conjugate was used in this test. |
