Phospho-c-Kit (Tyr719) Antibody【科瑞奥博】

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Phospho-c-Kit (Tyr719) Antibody

货号： 3391T 基本售价： 1600.0 元规格： -

产品信息

概述
货号	3391T
反应种属	Human/Mouse
来源宿主	Rabbit
应用	W
目标/特异性	Phospho-c-Kit (Tyr719) Antibody detects endogenous levels of c-Kit only when phosphorylated at tyrosine 719. The antibody cross-reacts with tyrosine-phosphorylated PDGF receptor.
使用方法	WB(1:1000)

性能
供应商	CST
灵敏度	Endogenous
背景	c-Kit is a member of the subfamily of receptor tyrosine kinases that includes PDGF, CSF-1, and FLT3/flk-2 receptors (1,2). It plays a critical role in activation and growth in a number of cell types including hematopoietic stem cells, mast cells, melanocytes, and germ cells (3). Upon binding with its stem cell factor (SCF) ligand, c-Kit undergoes dimerization/oligomerization and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components including PLCγ, the p85 subunit of PI3 kinase, SHP2, and CrkL (4). Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders (5), and mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors (6). Tyr719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo (7).
存放说明	-20C
计算分子量	120, 145
参考文献	1 . Martin, F.H. et al. (1990) Cell 63, 203-11. 2 . Yarden, Y. et al. (1987) EMBO J 6, 3341-51. 3 . Gommerman, J.L. et al. (1997) J Biol Chem 272, 30519-25. 4 . Sattler, M. et al. (1997) J Biol Chem 272, 10248-53. 5 . Nocka, K. et al. (1990) EMBO J 9, 1805-13. 6 . Hirota, S. et al. (1998) Science 279, 577-80. 7 . Blume-Jensen, P. et al. (2000) Nat Genet 24, 157-62.

参考图片

Western blot analysis of extracts from MO7e cells, untreated or stimulated with SCF #9907 (50 ng/ml for 2 minutes), using Phospho-c-Kit (Tyr719) Antibody (upper) or c-Kit Antibody #3392 (lower).用Phospho-c-Kit (Tyr719) Antibody (上)或 c-Kit Antibody #3392 (下)对经过或未经SCF#9907 (50 ng/ml ， 2 分钟)刺激的MO7e细胞进行免疫印迹检测。