| 货号 | MAB814R-01M |
| 简介 | Recombinant Monoclonal Antibody |
| 别名 | Fibroblast interferon; IFBIFNB; IFF; IFNB1; IFNbeta; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956 | 全称 | Interferon beta |
| 应用 | Neutralization |
| 目标/特异性 | Detects human IFN‑ beta in direct ELISAs. |
| 使用方法 | Neutralization: Measured by its ability to neutralize IFN-beta inhibition of EMCV-inducedcytopathy in the HeLa human cervicalepithelial carcinoma cell line. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens,M.J. et al. (eds): IRL Press. 129. The Neutralization Dose (ND50) is typically 7-21μg/mL in the presence of50 pg/mL Recombinant Human IFN-beta. |
| 来源 | Recombinant Monoclonal Mouse IgG1 Clone # 76703R |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3456 (Human); 15977 (Mouse); 24481 (Rat) |
| 纯化方式 | Protein A or G purified from cell culture supernatant |
| 免疫原 | E. coli-derived recombinant human IFN‑ beta Met22-Asn187 Accession # P01574 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 背景 | Interferon beta (IFN-beta ) is synthesized and secreted by fibroblasts and many other cell types in response to pathogens. IFN-beta binding to type I interferon receptors induces the upregulation of IRF-7 and activation of Rnase L. IRF-7 can exert a positive feedback on IFN-beta production. RNase L cleaves both viral and cellular single stranded mRNA, thereby limiting viral replication and dissemination. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
|
IFN-beta Inhibition of EMCV-inducedCytopathy andNeutralization by Human IFN-beta Antibody. Recombinant HumanIFN-beta (Catalog # 8499-IF)reduces theEncephalomyocarditis Virus(EMCV)-inducedcytopathy in theHeLa human cervical epithelialcarcinoma cell line in a dose-dependentmanner (orange line),as measured by crystal violetstaining. Inhibition of EMCVactivity elicited by RecombinantHuman IFN-beta (50 pg/mL) isneutralized (green line) byincreasing concentrations of Recombinant Human IFN-beta MonoclonalAntibody (Catalog # MAB814R).The ND50 is typically 7-21μg/mL. |
