Human IFN-beta MAb (Clone 76703) (500 UG)【科瑞奥博】

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Human IFN-beta MAb (Clone 76703) (500 UG)

货号： MAB814-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB814-500
别名	Fibroblast interferon; IFBIFNB; IFF; IFNB1; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956
全称	Interferon beta
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human IFN-beta in Western blots. In Western blots, this antibody does not cross-react with recombinant human IFN‑ alpha.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize IFN‑ beta inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The Neutralization Dose (ND₅₀) is typically 7-21 µg/mL in the presence of 50 pg/mL Recombinant Human IFN‑ beta.
来源	Monoclonal Mouse IgG₁ Clone # 76703
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3456 (Human); 15977 (Mouse); 24481 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Constitutively Active MAVS Inhibits HIV-1 Replication via Type I Interferon Secretion and Induction of HIV-1 Restriction Factors. Authors: Gupta S, Termini J, Issac B, Guirado E, Stone G PLoS ONE, 2015;11(2):e0148929. Species: Human Sample Type: Whole Cells Application: Neut Sialylation of Campylobacter jejuni endotoxin promotes dendritic cell-mediated B cell responses through CD14-dependent production of IFN-beta and TNF-alpha. Authors: Huizinga R, van Rijs W, Bajramovic J, Kuijf M, Laman J, Samsom J, Jacobs B J Immunol, 2013;191(11):5636-45. Species: Human Sample Type: Cell Culture Supernates Application: Neut An assessment of biological potency and molecular characteristics of different innovator and noninnovator interferon-beta products. Authors: Meager A, Dolman C, Dilger P, Bird C, Giovannoni G, Schellekens H, Thorpe R, Wadhwa M J. Interferon Cytokine Res., 2011;31(4):383-92. Species: Human Sample Type: IFN-beta-1a Application: WB
纯化方式	Protein A or G purified from ascites
免疫原	E. coli-derived recombinant human IFN-beta Met22-Asn187 Accession # P01574
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Interferon beta (IFN-beta ) is synthesized and secreted by fibroblasts and many other cell types in response to pathogens. IFN-beta binding to type I interferon receptors induces the upregulation of IRF-7 and activation of Rnase L. IRF-7 can exert a positive feedback on IFN-beta production. RNase L cleaves both viral and cellular single stranded mRNA, thereby limiting viral replication and dissemination.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Conventional/Classical Dendritic Cells Cytokines and Receptors in Angiogenesis IL-10/Interferon Family Infectious Diseases Plasmacytoid Dendritic Cells Th9 Cells

参考图片

IFN‑ beta Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ beta Antibody. Recombinant Human IFN‑ beta (Catalog # 8499-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN‑ beta (50 pg/mL) is neutralized (green line) by increasing concentrations of Human IFN‑ beta Monoclonal Antibody (Catalog # MAB814). The ND₅₀ is typically 7-21 µg/mL.