Human TNF-alpha MAb (Clone 6401) (500 UG)【科瑞奥博】

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Human TNF-alpha MAb (Clone 6401) (500 UG)

货号： MAB2101-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB2101-500
别名	APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; tnfa; tnf-a; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha
全称	Tumor Necrosis Factor alpha
反应种属	Human
应用	Neutralization
目标/特异性	Detects human TNF-alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 25‑50% cross‑reactivity with recombinant porcine TNF‑ alpha and recombinant rhesus macaque TNF-alpha is observed but no cross-reactivity with recombinant cotton rat TNF‑ alpha, recombinant rat TNF‑ alpha, recombinant human (rh) LT alpha 1/ beta 2, rhLT alpha 2/ beta 1, rhAPRIL, rhBAFF, rhEDA‑A2, recombinant mouse EDA, rhFas Ligand, rhLIGHT, rhOX40 Ligand, rhTRAIL, rhTRANCE, rhTWEAK, or rhVEGI is observed.
使用方法	Neutralization: Measured by its ability to neutralize TNF‑ alpha -induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND₅₀) is typically 0.015-0.06 µg/mL in the presence of 0.25 ng/mL Recombinant Human TNF‑ alpha and 1 µg/mL actinomycin D.
来源	Monoclonal Mouse IgG₁ Clone # 6401
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses Authors: Wan-Chien Cheng Eur J Immunol, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: Neut Vitamin D regulates cytokine patterns secreted by dendritic cells to promote differentiation of IL-22-producing T cells. Authors: Sommer A, Fabri M PLoS ONE, 2015;10(6):e0130395. Species: Human Sample Type: Whole Cells Application: Neut Innate lymphoid cells integrate stromal and immunological signals to enhance antibody production by splenic marginal zone B cells. Authors: Magri G, Miyajima M, Bascones S, Mortha A, Puga I, Cassis L, Barra C, Comerma L, Chudnovskiy A, Gentile M, Llige D, Cols M, Serrano S, Arostegui J, Juan M, Yague J, Merad M, Fagarasan S, Cerutti A Nat Immunol, 2014;15(4):354-64. Species: Human Sample Type: Whole Cells Application: Neut Production of interleukin 22 but not interleukin 17 by a subset of human skin-homing memory T cells. Authors: Duhen T, Geiger R, Jarrossay D, Lanzavecchia A, Sallusto F Nat. Immunol., 2009;10(8):857-63. Species: Human Sample Type: Whole Cells Application: Neut Der p 1 suppresses indoleamine 2, 3-dioxygenase in dendritic cells from house dust mite-sensitive patients with asthma. Authors: Maneechotesuwan K, Wamanuttajinda V, Kasetsinsombat K, Huabprasert S, Yaikwawong M, Barnes PJ, Wongkajornsilp A J. Allergy Clin. Immunol., 2009;123(1):239-48. Species: Human Sample Type: Whole Cells Application: Flow A novel mechanism of CD40-induced apoptosis of carcinoma cells involving TRAF3 and JNK/AP-1 activation. Authors: Georgopoulos NT, Steele LP, Thomson MJ, Selby PJ, Southgate J, Trejdosiewicz LK Cell Death Differ., 2006;13(10):1789-801. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from ascites
免疫原	E. coli-derived recombinant human TNF-alpha Gly57-Leu233 (predicted) Accession # P01375
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	TNF-alpha is a trimeric glycoprotein active in both membrane bound and secreted forms. TNF-alpha is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. TNF-alpha binds to TNF RI and TNF RII present on virtually all cell types where it triggers the activation of multiple signal transduction pathways and modulates the expression of a wide variety of genes.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Adipocytokines Cancer Biomarkers Conventional/Classical Dendritic Cells Co-stimulatory and Co-inhibitory Molecules Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis Cytokines in Lipid/Cholesterol Metabolism Diabetes Diabetic Peripheral Neuropathy EMT Induction gamma delta T Cells ICC / IHC Images: TNF-alpha Inflammatory Mediators Inflammatory/Monocyte-derived Dendritic Cells Interactive Pathway: Adipocytokines & Insulin Signaling Isolation, Culture, and Identification of Natural Killer Cells Macrophage Activation Markers Natural Killer T (NKT) Cells Neuroinflammation Obesity Plasmacytoid Dendritic Cells Preeclampsia Regulation of Apoptosis by TNF Superfamily Members Regulation of Natural Killer Cell Co-stimulation by TNF Superfamily Members Regulation of T Cell Co-stimulation by TNF Superfamily Members T Cell Cytokine Signaling Technical Notes: R&D Systems Is With You Every Step of the Way - Analyzing Proteins of Interest Th1 Cells Th22 Cells TNF Superfamily Ligands

参考图片

Cytotoxicity Induced by TNF‑ alpha and Neutralization by Human TNF‑ alpha Antibody. Recombinant Human TNF‑ alpha (Catalog # 210‑TA) induces cytotoxicity in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF‑ alpha (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Human TNF‑ alpha Monoclonal Antibody (Catalog # MAB2101). The ND₅₀ is typically 0.015-0.06 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).