Human CCL22/MDC MAb (Clone 57226) (500 UG)【科瑞奥博】

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Human CCL22/MDC MAb (Clone 57226) (500 UG)

货号： MAB336-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB336-500
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB336-500；Detection antibody：BAF336; and protein: 336-MD-025
别名	A-152E5.1; ABCD-1; CC chemokine STCP-1; C-C motif chemokine 22; chemokine (C-C motif) ligand 22; DC/B-CK; Macrophage-derived chemokine; MDC; MDCStimulated T-cell chemotactic protein 1; MGC34554; SCYA22MDC(1-69); small inducible cytokine A22; small inducible cytokine subfamily A (Cys-Cys), member 22; Small-inducible cytokine A22; STCP-1; stimulated T cell chemotactic protein 1
反应种属	Human
应用	Western Blot(1 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects human CCL22/MDC in ELISAs and Western blots. In ELISAs, no cross-reactivity with recombinant mouse CCL17, recombinant human (rh) CCL17, or rhCCL21 is observed.
使用方法	Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize CCL22/MDC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR4. The Neutralization Dose (ND₅₀) is typically 0.6-3.0 µg/mL in the presence of 10 ng/mL Recombinant Human CCL22/MDC.
来源	Monoclonal Mouse IgG_2B Clone # 57226
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6367 (Human); 20299 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver. Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH J. Immunol., 2010;184(6):2886-98. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded CCL17 and CCL22 chemokines within tumor microenvironment are related to accumulation of Foxp3+ regulatory T cells in gastric cancer. Authors: Mizukami Y, Kono K, Kawaguchi Y, Akaike H, Kamimura K, Sugai H, Fujii H Int. J. Cancer, 2008;122(10):2286-93. Species: Human Sample Type: Whole Cells Application: Flow Specific recruitment of regulatory T cells into the CSF in lymphomatous and carcinomatous meningitis. Authors: Haas J, Schopp L, Storch-Hagenlocher B, Fritzsching B, Jacobi C, Milkova L, Fritz B, Schwarz A, Suri-Payer E, Hensel M, Wildemann B Blood, 2007;111(2):761-6. Species: Human Sample Type: Whole Cells Application: Neut Generation of Th1 and Th2 chemokines by human eosinophils: evidence for a critical role of TNF-alpha. Authors: Liu LY, Bates ME, Jarjour NN, Busse WW, Bertics PJ, Kelly EA J. Immunol., 2007;179(7):4840-8. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Specific recruitment of regulatory T cells in ovarian carcinoma fosters immune privilege and predicts reduced survival. Authors: Curiel, Tyler J, Coukos, George, Zou, Linhua, Alvarez, Xavier, Cheng, Pui, Mottram, Peter, Evdemon-Hogan, Melina, Conejo-Garcia, Jose R, Zhang, Lin, Burow, Matthew, Zhu, Yun, Wei, Shuang, Kryczek, Ilona, Daniel, Ben, Gordon, Alan, Myers, Leann, Lackner, Andrew, Disis, Mary L, Knutson, Keith L, Chen, Lieping, Zou, Weiping Nat Med, 2004;10(9):942-9. Species: Human Sample Type: Whole Cells Application: Neut Enhanced generation of helper T type 1 and 2 chemokines in allergen-induced asthma. Authors: Liu L, Jarjour NN, Busse WW, Kelly EA Am. J. Respir. Crit. Care Med., 2004;169(10):1118-24. Species: Human Sample Type: BALF Application: ELISA Development CCR4 blockade does not inhibit allergic airways inflammation. Authors: Conroy DM, Jopling LA, Lloyd CM, Hodge MR, Andrew DP, Williams TJ, Pease JE, Sabroe I J. Leukoc. Biol., 2003;74(4):558-63. Species: Guinea Pig Sample Type: BALF Application: ELISA Development
纯化方式	Protein A or G purified from ascites
免疫原	E. coli-derived recombinant human CCL22/MDC Gly25-Gln93 Accession # O00626.1
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CCL22, also named stimulated T cell chemotactic protein (STCP-1) and MDC, is a CC chemokine initially isolated from clones of monocyte-derived macrophages. Human CCL22 cDNA encodes a precursor protein of 93 amino acid residues with a 24 amino acid residue predicted signal peptide that is cleaved to yield a 69 amino acid residue mature 8 kDa protein. At the amino acid sequence level, CCL22 shows less than 35% identity to other CC chemokine family members. Human CCL22 is expressed in dendritic cells, macrophages and activated monocytes. In addition, CCL22 expression is also detected in the tissues of thymus, lymph node and appendix. The gene for human CCL22 has been mapped to chromosome 16 rather than chromosome 17 where the genes for many human CC chemokines are clustered. Recombinant or chemically synthesized mature CCL22 has been shown to induce chemotaxis or Ca²⁺mobilization in dendritic cells, IL-2 activated NK cells, and activated T lymphocytes. A CD8⁺ T lymphocyte-derived secreted soluble activity that suppresses infection by primary non-syncytium-inducing and syncytium-inducing HIV-1 isolates and the T cell line-adapted isolate HIV-1_IIIB, has been identified as CCL22. Based on amino-terminal sequence analysis, the major CD8⁺ T lymphocyte-derived CCL22 protein yielded an amino-terminal sequence of YGANM, which is two amino acid residues shorter than the predicted mature CCL22. The difference in potency between the two mature CCL22 isoforms has not been determined.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Godiska, R. et al. (1997) J. Exp. Med. 185:1595. Chang, M-S.et al. (1997) J. Biol. Chem. 272:25229. Pal, R. et al. (1997) Science 278:5338.

参考图片

Chemotaxis Induced by CCL22/MDC and Neutralization by Human CCL22/MDC Antibody. Recombinant Human CCL22/MDC (Catalog # 336-MD) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL22/MDC (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL22/MDC Monoclonal Antibody (Catalog # MAB336). The ND₅₀ is typically 0.6-3.0 µg/mL.