| 货号 | MAB228-500 |
| 别名 | CD130 antigen; CD130; CDw130; DKFZp564F053; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form; GP130; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6RB; IL-6R-beta; IL6ST; interleukin 6 signal transducer (gp130, oncostatin M receptor); interleukin receptor beta chain; interleukin-6 receptor subunit beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; membrane glycoprotein gp130; Oncostatin-M receptor subunit alpha | 全称 | Glycoprotein 130 |
| 反应种属 | Human |
| 应用 | Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects human gp130 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2 R, rhIL-4 R, or rhIL-6 R is observed. |
| 使用方法 | Flow Cytometry: 2.5 µg/106cells Neutralization: Measured by its ability to neutralize Oncostatin M/OSM-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.04‑0.2 µg/mL in the presence of 0.8 ng/mL Recombinant Human Oncostatin M/OSM. |
| 来源 | Monoclonal Mouse IgG1 Clone # 28126 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3572 (Human); 16195 (Mouse); 25205 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Interleukin-6 in the bone marrow microenvironment promotes the growth and survival of neuroblastoma cells. | |
| 纯化方式 | Protein A or G purified from ascites |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human gp130 extracellular domain |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Glycoprotein 130 (gp130; also known as IL-6 signal transducer, IL-6 receptor beta, oncostatin-M alpha subunit) is a ubiquitously expressed, 130 kDa type I transmembrane glycoprotein and member of the type II subfamily, type I cytokine receptor family. Functionally, it is responsible for transduction of the IL-6 signal across the plasma membrane (1). Rat gp130 is synthesized as a 918 amino acid (aa) precursor with a 22 aa signal sequence, a 596 aa extracellular domain (ECD), a 22 aa transmembrane region, and a 278 aa cytoplasmic tail. Eleven potential N-linked glycosylation sites are found within the rat gp130 ECD (1). The ECD also contains an N terminal immunoglobulin (Ig)-like C2-type domain, followed by the cytokine receptor homology region (CHR) which is made up of two fibronectin type III-like domains and a WSXWS motif, and three additional fibronectin type III-like domains (2). The domains in the CHR are the structural hallmarks of the hematopoietic cytokine receptor family (2). Human gp130 shares 73% and 79% aa sequence identity with mouse and rat gp130, respectively. Gp130 serves as the signal transducing receptor subunit for the IL-6-type cytokines consisting of interleukin (IL)-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF), new neurotrophin factor-1 (NNT-1), IL-27, cardiotrophin-1 (CT-1), and cardiotrophin like cytokine (CLC) (2 - 5). These cytokines are involved in a variety of functions including the modulation of inflammatory and immune responses, heart development, fertility, and many other activities (2). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
|
Cell Proliferation Induced by Oncostatin M/OSM and Neutralization by Human gp130 Antibody. Recombinant Human Oncostatin M/OSM stimulates proliferation in the TF‑1 human erythroleukemic cell line (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human Oncostatin M/OSM (0.8 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human gp130 Monoclonal Antibody (Catalog # MAB228). The ND50 is typically 0.04-0.2 µg/mL. |
