Human gp130 MAb (Clone 28105) (500 UG)【科瑞奥博】

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Human gp130 MAb (Clone 28105) (500 UG)

货号： MAB628-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB628-500
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB628-500；Detection antibody：BAF228; and protein: 228-GP-010
别名	CD130 antigen; CD130; CDw130; DKFZp564F053; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form; GP130; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6RB; IL-6R-beta; IL6ST; interleukin 6 signal transducer (gp130, oncostatin M receptor); interleukin receptor beta chain; interleukin-6 receptor subunit beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; membrane glycoprotein gp130; Oncostatin-M receptor subunit alpha
全称	Glycoprotein 130
反应种属	Human
应用	ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects human gp130 in ELISAs. In sandwich immunoassays, no significant cross-reactivity or interference with recombinant human (rh) CNTF, rhIL-6, rhIL-6 R, recombinant mouse (rm) IL-6, rhIL-11, rhLIF, rhLIF R, rmLIF, or rhOSM is observed. This antibody blocks the human gp130-mediated bioactivities induced by IL-6, IL-11, LIF, OSM, and CNTF.
使用方法	ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize Oncostatin M/OSM-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 0.02-0.1 µg/mL in the presence of 0.8 ng/mL Recombinant Human Oncostatin M/OSM.
来源	Monoclonal Mouse IgG₁ Clone # 28105
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3572 (Human); 16195 (Mouse); 25205 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Immunocytochemical detection and RT-PCR expression of leukaemia inhibitory factor and its receptor in human fetal and adult ovaries. Authors: Abir R, Fisch B, Jin S, Barnnet M, Freimann S, van den Hurk R, Feldberg D, Nitke S, Krissi H, Ao A Mol. Hum. Reprod., 2004;10(5):313-9. Species: Human Sample Type: Whole Tissue Application: IHC Fresh/Frozen
纯化方式	Protein A or G purified from ascites
免疫原	Sf 21-derived recombinant human gp130 extracellular domain Leu24-Glu619 (Glu619-Asp, predicted) Accession # P40189
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	gp130, also known as CD130, belongs to the class I cytokine receptor family. It functions as a signal transducing receptor component in the receptor complexes of the IL-6 family of cytokines including IL-6, IL-11, LIF, OSM, CNTF and CT-1.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis Cytokines and Receptors in Tfh Cell Differentiation and Function Diabetes Human CD Marker Products ICC / IHC Images: gp130 IL-12 Family IL-6 Family Mini-review: Interleukin 6 Myeloid-derived Suppressor Cells (MDSC) Obesity Receptors in the Jak/STAT Pathway T Cell Cytokine Signaling Th1 Cells Th17 Cells VPS10P-domain Family

参考图片

Cell Proliferation Induced by Oncostatin M/OSM and Neutralization by Human gp130 Antibody. Recombinant Human Oncostatin M/OSM stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human Oncostatin M/OSM (0.8 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human gp130 Monoclonal Antibody (Catalog # MAB628). The ND₅₀ is typically 0.02-0.1 µg/mL.