| 货号 | MAB1975-500 |
| 别名 | FIL1 zeta; FIL1; FIL1(ZETA); FIL1ZFIL1 zeta; IL-1F7; IL-1F7IL-1 zeta; IL1H4IL1F7 (canonical product IL-1F7b); IL-1H4IL-1F7b (IL-1H4, IL-1H, IL-1RP1); IL-1RP1IL-1X protein; IL1RP1interleukin 1 family member 7; IL-1X; interleukin 1 family, member 7 (zeta); interleukin 1, zeta; interleukin-1 family member 7; Interleukin-1 homolog 4; interleukin-1 superfamily z; Interleukin-1 zeta; Interleukin-1-related protein | 全称 | Interleukin 37 |
| 反应种属 | Human |
| 应用 | Blockade of Receptor-ligand Interaction |
| 目标/特异性 | Detects human IL-37/IL-1F7 in direct ELISAs. In direct ELISAs, no cross‑reactivity with recombinant human (rh) IL-1 alpha, rhIL-1 beta, rhIL-1ra, rhIL-36Ra/IL-1F5, rhIL-36 alpha/IL-1F6, rhIL-36 beta/IL-1F8, or rhIL-18/IL-1F4 is observed. |
| 使用方法 | Blockade of Receptor-ligand Interaction: In a functional ELISA, 2-6 µg/mL of this antibody will block 50% of the binding of 1 μg/mL of Recombinant Human IL-18 R alpha /IL-1 R5 Fc Chimera (Catalog # 816-LR) to immobilized Recombinant Human IL-37/IL-1F7 (Catalog # 1975-IL) coated at 100 ng/mL (100 µL/well). At 50 μg/mL, this antibody will block >90% of the binding. |
| 来源 | Monoclonal Mouse IgG2B Clone # 261506 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 27178 (Human) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human IL-37/IL-1F7 Lys27-Asp192 Accession # NP_775297 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Human interleukin 1 family member 7 (IL-1F7), also named FIL-1Z, IL-1H4, and IL-1RP1, belongs to the IL-1 cytokine family, which currently has ten members. With the exception of IL-18 that maps to human chromosome 11, all other IL-1 family members map to the same cluster on human chromosome 2. Five alternatively spliced transcripts that arise through alternate exon usage have been described. These transcripts encode five different IL-1F7 isoforms (IL-1F7a through e also referred to as isofoms 1 through 5) that have distinct expression profiles. Polymorphism in the protein sequence of IL-1F7 isoforms also exists. Like IL-1 alpha, IL-1 beta and IL-18, all of the IL-1F7 variants lack a typical signal peptide. The longest IL-1F7 transcript, referred to as IL-1F7b or IL-1F7 isoform 1, encodes a 218 amino acid (aa) residues proprotein containing a 45 aa propeptide, which is removed by caspase-1 to generate the 173 aa mature segment. Mature IL-1F7b and other IL-1F7 variants lack potential N-linked glycosylation sites. The secreted mature IL-17F7b was reported to exist as a nondisulfide linked homodimers in solution, IL-1F7 shares approximately 21%, 24%, and 30% aa sequence identity with mature IL-1 alpha, IL-1 beta and IL-1ra, respectively. Mouse IL-1F7 has not been reported, but human IL-1F7 is active on mouse cells. IL-1F7b binds to IL-18 R alpha with low affinity but does not exert any IL-18 agonistic or antagonistic effects. IL-1F7b also binds to the IL-18BP to enhance the antagonistic effects of IL-18BP. It has been proposed that IL-1F7b form a trimeric complex with IL-18BP and IL-18 R beta. This complex blocks IL-18 activity by sequestering the signal transducing subunit and preventing it from participating in IL-18 signaling (1‑8). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
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