Human IL-4 R alpha MAb (Clone 25463) (500 UG)【科瑞奥博】

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Human IL-4 R alpha MAb (Clone 25463) (500 UG)

货号： MAB230-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB230-500
别名	CD124 antigen; CD124; IL-4 receptor subunit alpha; IL-4R subunit alpha; IL4R; IL-4RA; IL4RACD124; IL-4R-alpha; interleukin 4 receptor; interleukin-4 receptor alpha chain; interleukin-4 receptor subunit alpha
全称	Interleukin 4 Receptor alpha
反应种属	Human
应用	Western Blot(1 µg/mL) Flow Cytometry(0.25 µg/10⁶cells) Immunohistochemistry(8-25 µg/mL)
目标/特异性	Detects human IL‑4 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-9 R, recombinant mouse IL-4 R, rhIL-5 R alpha, rhIL-5 R beta, rhIL-13 R alpha 1, or rhIL-13 R alpha 2 is observed.
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 0.25 µg/10⁶cells Immunohistochemistry: 8-25 µg/mL Neutralization: Measured by its ability to neutralize IL‑4-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 3-6 ng/mL in the presence of 0.2 ng/mL Recombinant Human IL‑4.
来源	Monoclonal Mouse IgG_2A Clone # 25463
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3566 (Human); 16190 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Cancer-initiating cells from colorectal cancer patients escape from T cell-mediated immunosurveillance in vitro through membrane-bound IL-4. Authors: Volonte A, Di Tomaso T, Spinelli M, Todaro M, Sanvito F, Albarello L, Bissolati M, Ghirardelli L, Orsenigo E, Ferrone S, Doglioni C, Stassi G, Dellabona P, Staudacher C, Parmiani G, Maccalli C J Immunol, 2014;192(1):523-32. Species: Human Sample Type: whole cells Application: IF IL-4 and IL-13 differentially regulate TLR-induced eosinophil-basophil differentiation of cord blood CD34+ progenitor cells. Authors: Reece P, Gauvreau G, Sehmi R, Denburg J PLoS ONE, 2014;9(6):e100734. Species: Human Sample Type: Whole Cells Application: Neut Absence of a role for interleukin-13 in inflammatory bowel disease. Authors: Biancheri P, Di Sabatino A, Ammoscato F, Facciotti F, Caprioli F, Curciarello R, Hoque S, Ghanbari A, Joe-Njoku I, Giuffrida P, Rovedatti L, Geginat J, Corazza G, MacDonald T Eur J Immunol, 2014;44(2):370-85. Construction and application of elastin like polypeptide containing IL-4 receptor targeting peptide. Authors: Sarangthem, Vijaya, Cho, Eun A, Bae, Sang Mun, Singh, Thoudam, Kim, Sun-Ji, Kim, Soyoun, Jeon, Won Bae, Lee, Byung-He, Park, Rang-Woo PLoS ONE, 2013;8(12):e81891. Species: Human Sample Type: whole cells Application: Blocking Platelet ITAM signaling is critical for vascular integrity in inflammation. Authors: Boulaftali, Yacine, Hess, Paul R, Getz, Todd M, Cholka, Agnieszk, Stolla, Moritz, Mackman, Nigel, Owens, A Philli, Ware, Jerry, Kahn, Mark L, Bergmeier, Wolfgang J Clin Invest, 2013;123(2):908-16. Species: Human Sample Type: whole cells Application: Binding Assay Hrs Recognizes a Hydrophobic Amino Acid Cluster in Cytokine Receptors during Ubiquitin-independent Endosomal Sorting. Authors: Amano Y, Yamashita Y, Kojima K, Yoshino K, Tanaka N, Sugamura K, Takeshita T J. Biol. Chem., 2011;286(17):15458-72. Species: Human Sample Type: Whole Cells Application: Flow Fatty acid amide hydrolase in prostate cancer: association with disease severity and outcome, CB1 receptor expression and regulation by IL-4. Authors: Thors L, Bergh A, Persson E, Hammarsten P, Stattin P, Egevad L, Granfors T, Fowler CJ PLoS ONE, 2010;5(8):. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response. Authors: Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, Ring J, Traidl-Hoffmann C, Albanesi C, Cavani A J. Allergy Clin. Immunol., 2009;123(1):59-66.e4. Species: Human Sample Type: Whole Cells Application: Neut Th2 cytokine-induced upregulation of 11beta-hydroxysteroid dehydrogenase-1 facilitates glucocorticoid suppression of proasthmatic airway smooth muscle function. Authors: Hu A, Fatma S, Cao J, Grunstein JS, Nino G, Grumbach Y, Grunstein MM Am. J. Physiol. Lung Cell Mol. Physiol., 2009;296(5):L790-803. Species: Human Sample Type: Whole Cells Application: Neut Interleukin-4 induces the activation and collagen production of cultured human intrahepatic fibroblasts via the STAT-6 pathway. Authors: Aoudjehane L, Pissaia A, Scatton O, Podevin P, Massault PP, Chouzenoux S, Soubrane O, Calmus Y, Conti F Lab. Invest., 2008;88(9):973-85. Species: Human Sample Type: Whole Cells Application: ICC Interleukin-4 induces human hepatocyte apoptosis through a Fas-independent pathway. Authors: Aoudjehane L, Podevin P, Scatton O, Jaffray P, Dusanter-Fourt I, Feldmann G, Massault PP, Grira L, Bringuier A, Dousset B, Chouzenoux S, Soubrane O, Calmus Y, Conti F FASEB J., 2007;21(7):1433-44. Species: Human Sample Type: Whole Cells Application: ICC Selective induction of eotaxin release by interleukin-13 or interleukin-4 in human airway smooth muscle cells is synergistic with interleukin-1beta and is mediated by the interleukin-4 receptor alpha-chain. Authors: Hirst SJ, Hallsworth MP, Peng Q Am. J. Respir. Crit. Care Med., 2002;165(8):1161-71. Species: Human Sample Type: Cell Lysates Application: WB Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells. Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA J. Immunol., 2002;169(9):5244-51. Species: Human Sample Type: Whole Cells Application: Flow Clonal Th2 cells associated with chronic hypereosinophilia: TARC-induced CCR4 down-regulation in vivo. Authors: de Lavareille A, Roufosse F, Schandene L, Stordeur P, Cogan E, Goldman M Eur. J. Immunol., 2001;31(4):1037-46. Species: Human Sample Type: Whole Cells Application: Neut A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases. Authors: Albanesi C, Scarponi C, Sebastiani S, Cavani A, Federici M, Sozzani S, Girolomoni G J. Leukoc. Biol., 2001;70(4):617-23. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Protein A or G purified from ascites
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑4 R Gly24-His232 Accession # P24394
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Interleukin 4 receptor (IL-4 R) is the transmembrane ligand binding subunit of the heterodimeric IL-4 receptor complex. In the type I receptor complex, IL-4 R associates with the common gamma chain, whereas in the type II receptor complex, it associates with IL-13 R alpha 1. IL-4 R is expressed by B cells, T cells, monocytes and macrophages.
运输条件	Blue Ice
存放说明	-20℃
参考文献	B Cell Activation B Cell Markers Common gamma Chain Receptor Family Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis Cytokines and Receptors in Tfh Cell Differentiation and Function Human CD Marker Products ICC / IHC Images: IL-4 R alpha Interactive Pathway: IL-4 Signaling and its Primary Biological Effects in Different Immune Cell Types Interactive Pathway: IL-4 Signaling Pathways Macrophage Activation Markers Myeloid-derived Suppressor Cells (MDSC) Receptors in the Jak/STAT Pathway Regulatory T Cells (Tregs) T Cell Cytokine Signaling Th17 Cells Th2 Cells Th9 Cells

参考图片

Detection of IL‑4 R alpha in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitts lymphoma cell line was stained with Mouse Anti-Human IL‑4 R alpha Monoclonal Antibody (Catalog # MAB230, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

IL‑4 R alpha in Human Lymph Node. IL‑4 R alpha was detected in immersion fixed paraffin-embedded sections of human lymph node using Mouse Anti-Human IL‑4 R alpha Monoclonal Antibody (Catalog # MAB230) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 R alpha Antibody. Recombinant Human IL‑4 (Catalog # 204-IL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑4 (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑4 R alpha Monoclonal Antibody (Catalog # MAB230). The ND₅₀ is typically 3-6 ng/mL.