Human TNF RI/TNFRSF1A MAb (Clone 16803) (500 UG)【科瑞奥博】

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Human TNF RI/TNFRSF1A MAb (Clone 16803) (500 UG)

货号： MAB225-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB225-500
别名	CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor
全称	Tumor Necrosis Factor Receptor I
反应种属	Human
应用	Western Blot,Flow Cytometry,CyTOF-ready,Neutralization
目标/特异性	Detects human TNF RI in Western blots. In Western blots, does not cross-react with recombinant human (rh) TNF RII, rmTNF RI, or rmTNF RII.
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 0.25 µg/10⁶cells Neutralization: Measured by its ability to neutralize TNF RI/TNFRSF1A-mediated inhibition of cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 0.008-0.04 µg/mL in the presence of 15 ng/mL Recombinant Human TNF RI/TNFRSF1A, 0.25 ng/mL Recombinant Human TNF‑ alpha, and 1 µg/mL actinomycin D.
来源	Monoclonal Mouse IgG₁ Clone # 16803
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7132 (Human); 21937 (Mouse); 25625 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. TNF? promotes CAR-dependent migration of leukocytes across epithelial monolayers Sci Rep, 2016;6(0):26321. Species: Human Sample Type: Whole Cells Application: Neut TNF? promotes CAR-dependent migration of leukocytes across epithelial monolayers Sci Rep, 2016;6(0):26321. Species: Human Sample Type: Whole Cells Application: Neut Circulating TNF receptors 1 and 2 are associated with the severity of renal interstitial fibrosis in IgA nephropathy. Authors: Sonoda Y, Gohda T, Suzuki Y, Omote K, Ishizaka M, Matsuoka J, Tomino Y PLoS ONE, 2015;10(4):e0122212. Species: Human Sample Type: Whole Tissue Application: IHC The transmembrane domains of TNF-related apoptosis-inducing ligand (TRAIL) receptors 1 and 2 co-regulate apoptotic signaling capacity. Authors: Neumann S, Bidon T, Branschadel M, Krippner-Heidenreich A, Scheurich P, Doszczak M PLoS ONE, 2012;7(8):e42526. Species: Mouse Sample Type: Whole Cells Application: Flow IL-6 cytoprotection in hyperoxic acute lung injury occurs via suppressor of cytokine signaling-1-induced apoptosis signal-regulating kinase-1 degradation. Authors: Kolliputi N, Waxman AB Am. J. Respir. Cell Mol. Biol., 2009;40(3):314-24. Species: Human Sample Type: Cell Lysates Application: WB Tumour necrosis factor alpha stimulates the production of monocyte chemoattractants by extravillous trophoblast cells via differential activation of MAPK pathways. Authors: Renaud SJ, Sullivan R, Graham CH Placenta, 2009;30(4):313-9. Species: Human Sample Type: Whole Cells Application: Flow Der p 1 suppresses indoleamine 2, 3-dioxygenase in dendritic cells from house dust mite-sensitive patients with asthma. Authors: Maneechotesuwan K, Wamanuttajinda V, Kasetsinsombat K, Huabprasert S, Yaikwawong M, Barnes PJ, Wongkajornsilp A J. Allergy Clin. Immunol., 2009;123(1):239-48. Species: Human Sample Type: Whole Cells Application: Flow Differential responses of FLIPLong and FLIPShort-overexpressing human myeloid leukemia cells to TNF-alpha and TRAIL-initiated apoptotic signals. Authors: Seal S, Hockenbery DM, Spaulding EY, Kiem HP, Abbassi N, Deeg HJ Exp. Hematol., 2008;36(12):1660-72. Species: Human Sample Type: Whole Cells Application: Flow Death receptor expression is associated with poor response to chemotherapy and shorter survival in metastatic ovarian carcinoma. Authors: Dong HP, Kleinberg L, Silins I, Florenes VA, Trope CG, Risberg B, Nesland JM, Davidson B Cancer, 2008;112(1):84-93. Species: Human Sample Type: Whole Cells Application: Flow Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription. Authors: Ebert EC, Mehta V Cell. Immunol., 2006;241(1):7-13. Species: Human Sample Type: Whole Cells Application: Flow A novel mechanism of CD40-induced apoptosis of carcinoma cells involving TRAF3 and JNK/AP-1 activation. Authors: Georgopoulos NT, Steele LP, Thomson MJ, Selby PJ, Southgate J, Trejdosiewicz LK Cell Death Differ., 2006;13(10):1789-801. Species: Human Sample Type: Whole Cells Application: Flow A role for tumor necrosis factor alpha in death of dopaminergic neurons following neural transplantation. Authors: Clarke DJ, Branton RL Exp. Neurol., 2002;176(1):154-62. Species: Rat Sample Type: Whole Cells Application: Neut Negative selection by apoptosis enriches progenitors in naive and expanded human umbilical cord blood grafts. Authors: Mizrahi K, Ash S, Peled T, Yaniv I, Stein J, Askenasy N Bone Marrow Transplant, 0;49(7):942-9. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from ascites
免疫原	E. coli-derived recombinant human TNF RI/TNFRSF1A Accession # NP_001056
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	TNF RI occurs both in membrane bound and soluble forms and functions asa receptor for TNF-alpha and TNF-beta. In the superfamily nomenclature, it isdesignated TNFRSF1A.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Adipocytokines Cell Structure and Signaling Molecules Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis Cytokines in Lipid/Cholesterol Metabolism Diabetes Diabetic Peripheral Neuropathy EMT Induction Endothelial Cell Markers Human CD Marker Products ICC / IHC Images: TNF RI/TNFRSF1A Neuroinflammation Obesity Regulation of Apoptosis by TNF Superfamily Members T Cell Cytokine Signaling TNF Superfamily Receptors

参考图片

TNF RI/TNFRSF1A Inhibition of TNF‑ alpha -induced Cytotoxicity and Neutralization by Human TNF RI/TNFRSF1A Antibody. Recombinant Human TNF RI/TNFRSF1A (Catalog # 372-R1) inhibits Recombinant Human TNF‑ alpha (Catalog #
210-TA) induced cytotoxicity in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of Recombinant Human TNF‑ alpha (0.25 ng/mL) activity elicited by Recombinant Human TNF RI/TNFRSF1A (15 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB225). The ND₅₀ is typically
0.008-0.04 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

Detection of TNF RI/TNFRSF1A in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB225, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).