Human CCL3/MIP-1 alpha MAb (Clone 14215) (500 UG)【科瑞奥博】

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Human CCL3/MIP-1 alpha MAb (Clone 14215) (500 UG)

货号： MAB670-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB670-500
别名	C-C motif chemokine 3; MIP1-(a); AI323804; chemokine (C-C motif) ligand 3; G0S19-1; LD78a; LD78alpha; MIP-1 alpha; Mip1a; MIP-1alpha; MIP1-alpha; Scya3
反应种属	Human
应用	Neutralization
目标/特异性	Detects human CCL3/MIP-1 alpha.
使用方法	Neutralization: Measured by its ability to neutralize CCL3/MIP‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND₅₀) is typically 3-10 µg/mL in the presence of 0.1 µg/mL Recombinant Human CCL3/MIP‑1 alpha isoform LD78a.
来源	Monoclonal Mouse IgG_2A Clone # 14215
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6348 (Human); 20302 (Mouse); 25542 (Rat); 448787 (Canine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. A multiplex immunoassay for human adipokine profiling. Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E Clin. Chem., 2010;56(0):1320. Species: Human Sample Type: Cell Culture Supernates Application: Luminex Development Changes in soluble factor-mediated CD8+ cell-derived antiviral activity in cynomolgus macaques infected with simian immunodeficiency virus SIVmac251: relationship to biological markers of progression. Authors: Dioszeghy V, Benlhassan-Chahour K, Delache B, Dereuddre-Bosquet N, Aubenque C, Gras G, Le Grand R, Vaslin B J. Virol., 2006;80(1):236-45. Species: Primate â€“ Macaca fascicularis (Cynomolgus/Crab-eating Monkey) Sample Type: Cell Culture Supernates Application: ELISA Development Virally stimulated plasmacytoid dendritic cells produce chemokines and induce migration of T and NK cells. Authors: Megjugorac NJ, Young HA, Amrute SB, Olshalsky SL, Fitzgerald-Bocarsly P J. Leukoc. Biol., 2004;75(3):504-14. Species: Human Sample Type: Application: Flow Effect of cognate human CD4+ T cell and endothelial cell interactions upon chemokine production. Authors: Tay SS, McCormack A, Rose ML Transplantation, 2004;78(7):987-94. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells. Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA J. Immunol., 2002;169(9):5244-51. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development
纯化方式	Protein A or G purified from ascites
免疫原	E. coli-derived recombinant human CCL3/MIP-1 alpha
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	The macrophage inflammatory proteins -1 alpha and -1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP-1 alpha refers to the products of several independently cloned cDNAs, including LD78, pL78, pAT464, and GOS19. These cDNAs all code for the same human protein that is a homologue of the murine MIP-1 alpha. Mature MIP-1 alpha and MIP-1 beta in both human and mouse share approximately 70% homology at the amino acid level. The MIP‑1 proteins are members of the beta (C-C) subfamily of chemokines. Both MIP-1 alpha and MIP-1 beta are monocyte chemoattractants in vitro. Additionally, the MIP-1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with MIP-1 alpha and MIP-1 beta preferentially attracting CD8⁺ and CD4⁺ T cells, respectively. MIP-1 alpha has also been shown to attract B cells as well as eosinophils. MIP-1 proteins have been reported to have multiple effects on hematopoietic precursor cells and MIP-1 alpha has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. The functional receptor for MIP-1 alpha has been identified as CCR1 and CCR5.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.

参考图片

Chemotaxis Induced by CCL3/MIP‑1 alpha and Neutralization by Human CCL3/MIP‑1 alpha Antibody. Recombinant Human
CCL3/MIP‑1 alpha (Catalog #
270-LD) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human
CCL3/MIP‑1 alpha (0.1 µg/mL) is neutralized (green line) by increasing concentrations of
Mouse Anti-Human CCL3/MIP‑1 alpha Monoclonal Antibody (Catalog # MAB670). The ND₅₀ is typically 3-10 µg/mL.