货号 | MAB5351-500 |
描述 | For ELISA the Antibody Pairs information:Capture antibody:MAB5351-500;Detection antibody:BAF535; and protein: 535-IN-025 |
别名 | 3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; interleukin 8; K60; LAI; LECT; MDNCF; MDNCFb-ENAP; member 8; MONAPGCP1; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1 | 全称 | Interleukin 8 |
反应种属 | Porcine |
应用 | Western Blot,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization |
目标/特异性 | Detects porcine CXCL8/IL‑8 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human(rh) CXCL1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12/SDF-1 alpha, 12/SDF-1 beta, rhCXCL13,recombinant mouse CXCL1, 2, 6, 9, 10, 12/SDF-1 alpha, rmCXCL13, recombinant rat (rr)CXCL1, rrCXCL3/CINC2 alpha, or rr3/CINC-2 beta is observed. |
使用方法 | Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize CXCL8/IL‑8-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 1-6 µg/mL in the presence of 50 ng/mL Recombinant Porcine CXCL8/IL‑8. |
来源 | Monoclonal Mouse IgG1 Clone # 105105 |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline) |
应用文献 | |
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The effect of activated protein C on plasma cytokine levels in a porcine model of acute endotoxemia. | |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | E. coli-derived recombinant porcine CXCL8/IL‑8 Ala26-Gln104 Accession # CAA43461 |
内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
生物活性 | Porcine |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
背景 | Interleukin 8 was originally discovered and purified independently by a number of laboratories as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T-lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce IL-8 in response to a wide variety of pro‑inflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. IL-8 is a member of the alpha (C-X-C) subfamily of chemokines, which also includes platelet factor 4, GRO, IP-10, etc. IL-8 is a potent chemoattractant for neutrophils. In addition, IL-8 also has a wide range of other pro‑inflammatory effects. IL-8 causes degranulation of neutrophil specific granules and azurophilic granules. IL-8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Besides neutrophils, IL-8 is also chemotactic for basophils, T cells and eosinophils. IL-8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. Recently, IL-8 was reported to be angiogenic both in vivoandin vitro. |
运输条件 | Blue Ice |
存放说明 | -20℃ |
参考文献 |
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Chemotaxis Induced by CXCL8/IL‑8 and Neutralization by Porcine CXCL8/IL‑8 Antibody. Recombinant Porcine CXCL8/IL‑8 (Catalog # 535-IN) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Porcine CXCL8/IL‑8 (50 ng/mL) is neutralized (green line) by increasing concentrations of Porcine CXCL8/IL‑8 Monoclonal Antibody (Catalog # MAB5351). The ND50 is typically 1-6 µg/mL. |