| 货号 | MAB516 |
| 描述 | For ELISA the Antibody Pairs information：Capture antibody：MAB516；Detection antibody：BAF516; and protein: 517-CB-025 or 516-CA-025 |
| 别名 | chemokine (C-X-C motif) ligand 3; CINC-2; CINC-2b; C-X-C motif chemokine 3; Dcip1; Gm1960; GRO gamma; GRO3 oncogene; GRO3; GROG; GRO-gamma; GRO-gamma(1-73); Growth-regulated protein gamma; Macrophage inflammatory protein 2-beta; melanoma growth stimulatory activity gamma; MGSA gamma; MIP2B; MIP-2b; MIP2-beta; SCYB3 |
| 反应种属 | Rat |
| 应用 | Western Blot,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization |
| 目标/特异性 | Detects rat CXCL3/CINC-2 alpha / beta in ELISAs and Western blots. In sandwich immunoassays, 27.9% cross‑reactivity with recombinant rat (rr) CINC-3 is observed and no cross-reactivity or interference with rrCINC‑1, recombinant mouse (rm) KC, rmMIP-2, or recombinant human GRO alpha is observed. |
| 使用方法 | Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : ELISA Standard : Neutralization: Measured by its ability to neutralize CXCL3/CINC‑2 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.5-2.5 µg/mL in the presence of 0.05 µg/mL Recombinant Rat CXCL3/CINC‑2 alpha. |
| 来源 | Monoclonal Mouse IgG2B Clone # 83504 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2921 (Human); 330122 (Mouse); 171551 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Rat coronaviruses infect rat alveolar type I epithelial cells and induce expression of CXC chemokines. | |
| 纯化方式 | Protein A or G purified from ascites |
| 免疫原 | E. coli-derived recombinant rat CXCL3/CINC-2 alpha / beta Arg33-Ser100 Accession # Q10746 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | CINC-2 alpha and -2 beta are mRNA splice variant isoforms that differ by only 3amino acids at their amino-termini. These proteins are CXC chemokinesmost closely related to human GROs and mouse MIP-2. They are producedby granulation tissue, activated macrophages and fibroblasts. CINC-2biological activities are mediated by CXCR1 or CXCR2. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
|
Chemotaxis Induced by CXCL3/CINC‑2 alpha and Neutralization by Rat CXCL3/CINC‑2 alpha / beta Antibody. Recombinant Rat CXCL3/ |
