| 货号 | MAB6049 |
| 简介 | Clone 683409 was used by HLDA to establish CD designation |
| 别名 | CD370; C-type lectin domain family 9 member A; C-type lectin domain family 9, member A; DNGR1; HEEE9341; UNQ9341 | 全称 | C-type Lectin-like Receptor 9 |
| 反应种属 | Human |
| 应用 | Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects human CLEC9a in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) CLEC1, 2, 2A, 3B, 10A, 12B, 14A, rhCD302/CLEC13a, rhMICL, or recombinant mouse CLEC9a is observed. |
| 使用方法 | Flow Cytometry: 2.5 µg/106cells Binding Inhibition: The binding of Recombinant Human CLEC9a Fc Chimera (Catalog # 6049-CL, 100 ng/mL) to a ligand expressed in Fas Ligand-treated A20 mouse B cell lymphoma cell line was maximally inhibited (>90%) by 1 µg/mL of the antibody, as detected by flow cytometry. |
| 来源 | Monoclonal Mouse IgG1 Clone # 683409 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 283420 (Human); 232414 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human CLEC9a Lys57-Val241 Accession # Q6UXN8 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.5 mg/mL. |
| 背景 | CLEC9a (C-type lectin domain family 9 member A), also known as DNGR-1, is a type II transmembrane glycoprotein member of the C‑type lectin superfamily (1, 2). Mature human CLEC9a consists of a 35 amino acid (aa) cytoplasmic domain with an ITAM-like motif, a 21 aa transmembrane segment, and a 185 extracellular domain (ECD) that contains a stalk region and one C-type lectin domain (CTLD) (3‑5). Within the ECD, human CLEC9a shares 57% aa sequence identity with mouse and rat CLEC9a. Although the CTLD of CLEC9a structurally resembles that of other C-type lectins, it lacks the conserved residues that typically mediate calcium and carbohydrate binding. CLEC9a is expressed as a disulfide-linked homodimer of approximately 50 kDa N-glycosylated subunits (3, 5). Human CLEC9a expression is restricted to a subpopulation of BDCA-3+ conventional dendritic cells (cDC) and CD16- monocytes (3‑5). BDCA-3+ cDC are analagous to mouse CD8+ cDC which are specialized in antigenic cross-presentation in complex with MHC class I molecules (6). In mouse, CLEC9a is additionally expressed on plasmacytoid dendritic cells (4, 5). CLEC9a ligation enhances antigen uptake and processing, leading to presentation on MHC class I and cytotoxic T cell (CTL) priming (3‑5). In mouse, CLEC9a recognizes normally intracellular determinant(s) of necrotic cells and mediates their uptake by the dendritic cell (7). The subsequent antigenic cross-presentation to CTL is important for clearing necrotic cellular debris (7). CLEC9a signaling triggers activation of the tyrosine kinase Syk (3, 7). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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